<i>Vibrio harveyi</i> Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, <i>Litopenaeus vannamei</i>
<i>Vibrio harveyi</i> is one of the pathogens that threaten the shrimp farming industry. However, metabolic changes induced by <i>V. harveyi infection</i> in shrimp remain unknown. In this study, we first conducted high resolution-magic angle spinning (HR-MAS) nuclear magneti...
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| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/6/265 |
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| author | Seohee Ma Ahran Kim Wonho Lee Seonghye Kim Sujin Lee Dahye Yoon Jin-Sol Bae Chan-Il Park Suhkmann Kim |
| author_facet | Seohee Ma Ahran Kim Wonho Lee Seonghye Kim Sujin Lee Dahye Yoon Jin-Sol Bae Chan-Il Park Suhkmann Kim |
| author_sort | Seohee Ma |
| collection | DOAJ |
| description | <i>Vibrio harveyi</i> is one of the pathogens that threaten the shrimp farming industry. However, metabolic changes induced by <i>V. harveyi infection</i> in shrimp remain unknown. In this study, we first conducted high resolution-magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR)-based metabolomics studies on gill, hepatopancreas, and haemolymph of <i>V. harveyi</i>-infected white leg shrimp, <i>Litopenaeus vannamei</i>. Using multivariate statistical analysis, we observed a clear separation between the early (3 and 9 h post-injection (hpi)) and late phases (24, 72 and 144 hpi) of the infection in all tissues. Moreover, metabolic changes in response to <i>V. harveyi</i> infection were faster in the haemolymph in the early phase and significantly changed in the late phase of the infection in the gills. Extensive changes were observed in the hepatopancreas, with 24 hpi being the turning point of progression from early to late phase infection in the hepatopancreas. <i>V. harveyi infection</i> increased the energy demand in <i>L. vannamei</i> and the amino acid and carbohydrate metabolism pathways also exhibited significant changes depending on the tissue. Thus, each tissue displayed different metabolic changes, depending on the progress of the infection. |
| first_indexed | 2024-03-10T18:53:21Z |
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| id | doaj.art-7b2e54db7597467b9f9fce0064db1ef4 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T18:53:21Z |
| publishDate | 2020-06-01 |
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| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-7b2e54db7597467b9f9fce0064db1ef42023-11-20T04:57:44ZengMDPI AGMetabolites2218-19892020-06-0110626510.3390/metabo10060265<i>Vibrio harveyi</i> Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, <i>Litopenaeus vannamei</i>Seohee Ma0Ahran Kim1Wonho Lee2Seonghye Kim3Sujin Lee4Dahye Yoon5Jin-Sol Bae6Chan-Il Park7Suhkmann Kim8Department of Chemistry, Center for Proteome Biophysics, and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, KoreaDepartment of Chemistry, Center for Proteome Biophysics, and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, KoreaDepartment of Chemistry, Center for Proteome Biophysics, and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, KoreaDepartment of Chemistry, Center for Proteome Biophysics, and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, KoreaDepartment of Chemistry, Center for Proteome Biophysics, and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, KoreaDepartment of Chemistry, Center for Proteome Biophysics, and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, KoreaDepartment of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, KoreaDepartment of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, KoreaDepartment of Chemistry, Center for Proteome Biophysics, and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Korea<i>Vibrio harveyi</i> is one of the pathogens that threaten the shrimp farming industry. However, metabolic changes induced by <i>V. harveyi infection</i> in shrimp remain unknown. In this study, we first conducted high resolution-magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR)-based metabolomics studies on gill, hepatopancreas, and haemolymph of <i>V. harveyi</i>-infected white leg shrimp, <i>Litopenaeus vannamei</i>. Using multivariate statistical analysis, we observed a clear separation between the early (3 and 9 h post-injection (hpi)) and late phases (24, 72 and 144 hpi) of the infection in all tissues. Moreover, metabolic changes in response to <i>V. harveyi</i> infection were faster in the haemolymph in the early phase and significantly changed in the late phase of the infection in the gills. Extensive changes were observed in the hepatopancreas, with 24 hpi being the turning point of progression from early to late phase infection in the hepatopancreas. <i>V. harveyi infection</i> increased the energy demand in <i>L. vannamei</i> and the amino acid and carbohydrate metabolism pathways also exhibited significant changes depending on the tissue. Thus, each tissue displayed different metabolic changes, depending on the progress of the infection.https://www.mdpi.com/2218-1989/10/6/265high resolution-magic angle spinningnuclear magnetic resonance spectroscopymetabolomics<i>Litopenaeus vannamei</i><i>Vibrio harveyi</i> |
| spellingShingle | Seohee Ma Ahran Kim Wonho Lee Seonghye Kim Sujin Lee Dahye Yoon Jin-Sol Bae Chan-Il Park Suhkmann Kim <i>Vibrio harveyi</i> Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, <i>Litopenaeus vannamei</i> Metabolites high resolution-magic angle spinning nuclear magnetic resonance spectroscopy metabolomics <i>Litopenaeus vannamei</i> <i>Vibrio harveyi</i> |
| title | <i>Vibrio harveyi</i> Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, <i>Litopenaeus vannamei</i> |
| title_full | <i>Vibrio harveyi</i> Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, <i>Litopenaeus vannamei</i> |
| title_fullStr | <i>Vibrio harveyi</i> Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, <i>Litopenaeus vannamei</i> |
| title_full_unstemmed | <i>Vibrio harveyi</i> Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, <i>Litopenaeus vannamei</i> |
| title_short | <i>Vibrio harveyi</i> Infection Significantly Alters Amino Acid and Carbohydrate Metabolism in Whiteleg Shrimp, <i>Litopenaeus vannamei</i> |
| title_sort | i vibrio harveyi i infection significantly alters amino acid and carbohydrate metabolism in whiteleg shrimp i litopenaeus vannamei i |
| topic | high resolution-magic angle spinning nuclear magnetic resonance spectroscopy metabolomics <i>Litopenaeus vannamei</i> <i>Vibrio harveyi</i> |
| url | https://www.mdpi.com/2218-1989/10/6/265 |
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