Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue
We have previously shown that implantation of testis cell aggregates under the back skin of immunodeficient mice results in de novo regeneration of testis tissue. We used this unique model to investigate the effects of epidermal growth factor (EGF) and glial cell-derived neurotrophic factor (GDNF) o...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Bioscientifica
2022-03-01
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| Series: | Reproduction and Fertility |
| Subjects: | |
| Online Access: | https://raf.bioscientifica.com/view/journals/raf/aop/raf-21-0057/raf-21-0057.xml |
| _version_ | 1819158057460957184 |
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| author | Awang Hazmi Awang-Junaidi Mohammad Amin Fayaz Savannah Goldstein Ali Honaramooz |
| author_facet | Awang Hazmi Awang-Junaidi Mohammad Amin Fayaz Savannah Goldstein Ali Honaramooz |
| author_sort | Awang Hazmi Awang-Junaidi |
| collection | DOAJ |
| description | We have previously shown that implantation of testis cell aggregates under the back skin of immunodeficient mice results in de novo regeneration of testis tissue. We used this unique model to investigate the effects of epidermal growth factor (EGF) and glial cell-derived neurotrophic factor (GDNF) on testis cord development. Neonatal piglet testis cells were briefly (<1 h) exposed to either low (L: 0.02 μg/mL) or high (H: 2 μg/mL) doses of EGF, GDNF, or vehicle (control), before implantation in recipient mice. Randomly selected implants were removed from each mouse at 1, 2, 4, and 8 weeks post-implantation. GDNF-L implants showed increased testis cord development over time, and EGF-L implants had increased cross-sectional area. The ratio of regular cords decreased over time in EGF-H and GDNF-H implants and was replaced by a higher ratio of irregular cords in GDNF-H. EGF-L and GDNF-H implants were quickest to display rete testis-like structures. Overall, the lower dose of each growth factor was more effective than its higher dose in improving the implantation outcomes. This is the first comprehensive assessment of these key growth factors on de novo formation (regeneration) of testis tissue. |
| first_indexed | 2024-12-22T16:18:36Z |
| format | Article |
| id | doaj.art-7b2f2180019c4941969b779e2fb74306 |
| institution | Directory Open Access Journal |
| issn | 2633-8386 |
| language | English |
| last_indexed | 2024-12-22T16:18:36Z |
| publishDate | 2022-03-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj.art-7b2f2180019c4941969b779e2fb743062022-12-21T18:20:18ZengBioscientificaReproduction and Fertility2633-83862022-03-01313956https://doi.org/10.1530/RAF-21-0057Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissueAwang Hazmi Awang-Junaidi0Mohammad Amin Fayaz1Savannah Goldstein2Ali Honaramooz3Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, CanadaDepartment of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, CanadaDepartment of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, CanadaDepartment of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, CanadaWe have previously shown that implantation of testis cell aggregates under the back skin of immunodeficient mice results in de novo regeneration of testis tissue. We used this unique model to investigate the effects of epidermal growth factor (EGF) and glial cell-derived neurotrophic factor (GDNF) on testis cord development. Neonatal piglet testis cells were briefly (<1 h) exposed to either low (L: 0.02 μg/mL) or high (H: 2 μg/mL) doses of EGF, GDNF, or vehicle (control), before implantation in recipient mice. Randomly selected implants were removed from each mouse at 1, 2, 4, and 8 weeks post-implantation. GDNF-L implants showed increased testis cord development over time, and EGF-L implants had increased cross-sectional area. The ratio of regular cords decreased over time in EGF-H and GDNF-H implants and was replaced by a higher ratio of irregular cords in GDNF-H. EGF-L and GDNF-H implants were quickest to display rete testis-like structures. Overall, the lower dose of each growth factor was more effective than its higher dose in improving the implantation outcomes. This is the first comprehensive assessment of these key growth factors on de novo formation (regeneration) of testis tissue.https://raf.bioscientifica.com/view/journals/raf/aop/raf-21-0057/raf-21-0057.xmlgrowth factorstestis cell implantationtestis cord developmenttestis tubulogenesismale reproduction |
| spellingShingle | Awang Hazmi Awang-Junaidi Mohammad Amin Fayaz Savannah Goldstein Ali Honaramooz Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue Reproduction and Fertility growth factors testis cell implantation testis cord development testis tubulogenesis male reproduction |
| title | Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue |
| title_full | Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue |
| title_fullStr | Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue |
| title_full_unstemmed | Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue |
| title_short | Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue |
| title_sort | brief exposure of neonatal testis cells to egf or gdnf alters the regenerated tissue |
| topic | growth factors testis cell implantation testis cord development testis tubulogenesis male reproduction |
| url | https://raf.bioscientifica.com/view/journals/raf/aop/raf-21-0057/raf-21-0057.xml |
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