Detection of Leptin Activity in Living Cells Expressing Chicken Leptin Receptor and STAT3
The chicken leptin receptor (chLEPR) activates Janus kinase (JAK) - signal transducers and activator of transcription (STAT) signaling pathway after leptin stimulation. We have previously developed a bioassay using leptin inducible reporter gene in cultured cells. However, we failed to detect leptin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2012-01-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/49/1/49_011085/_pdf/-char/en |
| _version_ | 1797816185891323904 |
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| author | Hiromi Adachi Daisuke Murase Susumu Atomura Takeshi Ohkubo |
| author_facet | Hiromi Adachi Daisuke Murase Susumu Atomura Takeshi Ohkubo |
| author_sort | Hiromi Adachi |
| collection | DOAJ |
| description | The chicken leptin receptor (chLEPR) activates Janus kinase (JAK) - signal transducers and activator of transcription (STAT) signaling pathway after leptin stimulation. We have previously developed a bioassay using leptin inducible reporter gene in cultured cells. However, we failed to detect leptin-like activity in chicken blood. In the present study, we expressed green fluorescent protein (GFP) fused chSTAT3 (GFP-chSTAT3) in cells expressing chLEPR and analyzed leptin dependent activation of the chSTAT3. Leptin phosphorylated GFP-chSTAT3 and by which fluorescent signal translocated into nuclei in COS-7 cells transiently expressing GFP-chSTAT3 with chLEPR. Furthermore, we established CHO-K1 cells stably expressing chLEPR and chSTAT3 (CHO-chLEPR/chSTAT3), and in which detected time- and dose-dependent activation of chSTAT3 by leptin. Therefore, the CHO-chLEPR/STAT3 cells would be an excellent tool to detect and monitor leptin-like activity in avian tissues. |
| first_indexed | 2024-03-13T08:34:01Z |
| format | Article |
| id | doaj.art-7b355073768c4d8b928513099dc065c4 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:34:01Z |
| publishDate | 2012-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-7b355073768c4d8b928513099dc065c42023-05-31T01:10:14ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862012-01-01491465010.2141/jpsa.011085jpsaDetection of Leptin Activity in Living Cells Expressing Chicken Leptin Receptor and STAT3Hiromi Adachi0Daisuke Murase1Susumu Atomura2Takeshi Ohkubo3College of Agriculture, Ibaraki University, JapanCollege of Agriculture, Ibaraki University, JapanCollege of Agriculture, Ibaraki University, JapanCollege of Agriculture, Ibaraki University, JapanThe chicken leptin receptor (chLEPR) activates Janus kinase (JAK) - signal transducers and activator of transcription (STAT) signaling pathway after leptin stimulation. We have previously developed a bioassay using leptin inducible reporter gene in cultured cells. However, we failed to detect leptin-like activity in chicken blood. In the present study, we expressed green fluorescent protein (GFP) fused chSTAT3 (GFP-chSTAT3) in cells expressing chLEPR and analyzed leptin dependent activation of the chSTAT3. Leptin phosphorylated GFP-chSTAT3 and by which fluorescent signal translocated into nuclei in COS-7 cells transiently expressing GFP-chSTAT3 with chLEPR. Furthermore, we established CHO-K1 cells stably expressing chLEPR and chSTAT3 (CHO-chLEPR/chSTAT3), and in which detected time- and dose-dependent activation of chSTAT3 by leptin. Therefore, the CHO-chLEPR/STAT3 cells would be an excellent tool to detect and monitor leptin-like activity in avian tissues.https://www.jstage.jst.go.jp/article/jpsa/49/1/49_011085/_pdf/-char/enbioassaychickenleptinsignal transductionstat3 |
| spellingShingle | Hiromi Adachi Daisuke Murase Susumu Atomura Takeshi Ohkubo Detection of Leptin Activity in Living Cells Expressing Chicken Leptin Receptor and STAT3 The Journal of Poultry Science bioassay chicken leptin signal transduction stat3 |
| title | Detection of Leptin Activity in Living Cells Expressing Chicken Leptin Receptor and STAT3 |
| title_full | Detection of Leptin Activity in Living Cells Expressing Chicken Leptin Receptor and STAT3 |
| title_fullStr | Detection of Leptin Activity in Living Cells Expressing Chicken Leptin Receptor and STAT3 |
| title_full_unstemmed | Detection of Leptin Activity in Living Cells Expressing Chicken Leptin Receptor and STAT3 |
| title_short | Detection of Leptin Activity in Living Cells Expressing Chicken Leptin Receptor and STAT3 |
| title_sort | detection of leptin activity in living cells expressing chicken leptin receptor and stat3 |
| topic | bioassay chicken leptin signal transduction stat3 |
| url | https://www.jstage.jst.go.jp/article/jpsa/49/1/49_011085/_pdf/-char/en |
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