Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters
The basic pathogenic step in the process of tumor growth, invasion and metastasis is tumor-induced angiogenesis. The aim of this study was to evaluate the angiogenesis in colorectal carcinoma by microvascular density (MVD) determination with IHC (immunohistochemistry) method and to determine if and...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2009-10-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14423.pdf&manuscript_id=14423 |
| _version_ | 1818454908087566336 |
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| author | Sharifi Nourieh Ghaffarzadegan Kamran Ayatollahi Hossein Shakeri Mohammad Taghi Sadeghian Mohammad Hadi Bordbar Azari Jafar |
| author_facet | Sharifi Nourieh Ghaffarzadegan Kamran Ayatollahi Hossein Shakeri Mohammad Taghi Sadeghian Mohammad Hadi Bordbar Azari Jafar |
| author_sort | Sharifi Nourieh |
| collection | DOAJ |
| description | The basic pathogenic step in the process of tumor growth, invasion and metastasis is tumor-induced angiogenesis. The aim of this study was to evaluate the angiogenesis in colorectal carcinoma by microvascular density (MVD) determination with IHC (immunohistochemistry) method and to determine if and how angiogenesis correlates with clinicopathologic parameters. Sixty two archival, paraffin embedded tissue samples of colorectal carcinoma from Omid Hospital (Mashhad, Islamic republic of Iran) were selected. Microvessels were identified immunohistochemically, using monoclonal CD34 antibody. Two investigators examined the microvessel density then the median value of MVD was determined and correlated with clinicopathologic parameters. Tumor-induced angiogenesis of colorectal carcinoma statistically correlated with histological tumor grade (P=0.000). There was no significant correlation between intratumoral microvessel density and sex and age of patients, localization, and stage and histologicalal tumor type (P > 0.1). Intratumoral microvessel density quantification in histologic specimens of colorectal carcinoma reflects the grade of tumors and may be a useful additional prognostic factor. |
| first_indexed | 2024-12-14T22:02:21Z |
| format | Article |
| id | doaj.art-7b383db3441b4c578d27a1cdbb63fdcf |
| institution | Directory Open Access Journal |
| issn | 0044-6025 |
| language | English |
| last_indexed | 2024-12-14T22:02:21Z |
| publishDate | 2009-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-7b383db3441b4c578d27a1cdbb63fdcf2022-12-21T22:45:57ZengTehran University of Medical SciencesActa Medica Iranica0044-60252009-10-01473161164Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic ParametersSharifi NouriehGhaffarzadegan KamranAyatollahi HosseinShakeri Mohammad TaghiSadeghian Mohammad HadiBordbar Azari JafarThe basic pathogenic step in the process of tumor growth, invasion and metastasis is tumor-induced angiogenesis. The aim of this study was to evaluate the angiogenesis in colorectal carcinoma by microvascular density (MVD) determination with IHC (immunohistochemistry) method and to determine if and how angiogenesis correlates with clinicopathologic parameters. Sixty two archival, paraffin embedded tissue samples of colorectal carcinoma from Omid Hospital (Mashhad, Islamic republic of Iran) were selected. Microvessels were identified immunohistochemically, using monoclonal CD34 antibody. Two investigators examined the microvessel density then the median value of MVD was determined and correlated with clinicopathologic parameters. Tumor-induced angiogenesis of colorectal carcinoma statistically correlated with histological tumor grade (P=0.000). There was no significant correlation between intratumoral microvessel density and sex and age of patients, localization, and stage and histologicalal tumor type (P > 0.1). Intratumoral microvessel density quantification in histologic specimens of colorectal carcinoma reflects the grade of tumors and may be a useful additional prognostic factor.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14423.pdf&manuscript_id=14423Angiogenesisantigens, CD34colorectal neoplasmimmunohistochemistryclinical pathology |
| spellingShingle | Sharifi Nourieh Ghaffarzadegan Kamran Ayatollahi Hossein Shakeri Mohammad Taghi Sadeghian Mohammad Hadi Bordbar Azari Jafar Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters Acta Medica Iranica Angiogenesis antigens, CD34 colorectal neoplasm immunohistochemistry clinical pathology |
| title | Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters |
| title_full | Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters |
| title_fullStr | Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters |
| title_full_unstemmed | Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters |
| title_short | Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters |
| title_sort | evaluation of angiogenesis in colorectal carcinoma by cd34 immunohistochemistry method and its correlation with clinicopathologic parameters |
| topic | Angiogenesis antigens, CD34 colorectal neoplasm immunohistochemistry clinical pathology |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14423.pdf&manuscript_id=14423 |
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