Knockdown Fascin impairs bone marrow mesenchymal stem cells migration capacity of Wuzhishan Miniature pig
The bone marrow mesenchymal stem cells (BM-MSCs) from Wuzhishan pig (WZSP) with knockdown expression of Fascin were used to make out the role Fascin gene in migratory potential of BM-MSCs. The role of Fascin gene is well established in cellular migration, but it is unclear for its activity in porci...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2014-10-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/44250 |
| _version_ | 1797655902931648512 |
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| author | LING YANG YING XIA XIAO-LI TAO WEI HE TIAN-WEN WU LEI HUANG JIN-XUE RUAN SHU-LIN YANG YU-LIAN MU KUI LI SHU-TANG FENG |
| author_facet | LING YANG YING XIA XIAO-LI TAO WEI HE TIAN-WEN WU LEI HUANG JIN-XUE RUAN SHU-LIN YANG YU-LIAN MU KUI LI SHU-TANG FENG |
| author_sort | LING YANG |
| collection | DOAJ |
| description |
The bone marrow mesenchymal stem cells (BM-MSCs) from Wuzhishan pig (WZSP) with knockdown expression of Fascin were used to make out the role Fascin gene in migratory potential of BM-MSCs. The role of Fascin gene is well established in cellular migration, but it is unclear for its activity in porcine BM-MSCs. At present study, BM-MSCs were isolated from femur and tibia of the WZSP with about 42 day-old, and had been evaluated by previous study. At first, 4 vectors encoding different short hairpin RNA (shRNA) for Fascin were designed to knock down Fascin, and qRT-PCR was employed to detect the expression of Fascin mRNA by BM-MSCs after transfection, and the vector with best interference effect was selected to be used in the following experiments. And then, the effect of knock down Fascin was further determined through Western blot using Fascin antibody. Finally, the migration capacity of the BM-MSCs was evaluated through scratch assay and transwell migration assay. The results showed that the specific shRNA for knocking down Fascin efficiently was found for the BM-MSCs from WZSP, and Fascin was involved in regulating the migration capacity of the BM-MSCs in vitro, which may be useful for the BM-MSCs from WZSP to be utilized in regenerative therapy for human.
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| first_indexed | 2024-03-11T17:21:42Z |
| format | Article |
| id | doaj.art-7b389aa40e984af38d0bc54ac19d3b31 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-11T17:21:42Z |
| publishDate | 2014-10-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-7b389aa40e984af38d0bc54ac19d3b312023-10-19T11:14:16ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272014-10-01841010.56093/ijans.v84i10.44250Knockdown Fascin impairs bone marrow mesenchymal stem cells migration capacity of Wuzhishan Miniature pigLING YANG0YING XIA1XIAO-LI TAO2WEI HE3TIAN-WEN WU4LEI HUANG5JIN-XUE RUAN6SHU-LIN YANG7YU-LIAN MU8KUI LI9SHU-TANG FENG10Hebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 ChinaHebei University of Engineering, Handan 056 021 China The bone marrow mesenchymal stem cells (BM-MSCs) from Wuzhishan pig (WZSP) with knockdown expression of Fascin were used to make out the role Fascin gene in migratory potential of BM-MSCs. The role of Fascin gene is well established in cellular migration, but it is unclear for its activity in porcine BM-MSCs. At present study, BM-MSCs were isolated from femur and tibia of the WZSP with about 42 day-old, and had been evaluated by previous study. At first, 4 vectors encoding different short hairpin RNA (shRNA) for Fascin were designed to knock down Fascin, and qRT-PCR was employed to detect the expression of Fascin mRNA by BM-MSCs after transfection, and the vector with best interference effect was selected to be used in the following experiments. And then, the effect of knock down Fascin was further determined through Western blot using Fascin antibody. Finally, the migration capacity of the BM-MSCs was evaluated through scratch assay and transwell migration assay. The results showed that the specific shRNA for knocking down Fascin efficiently was found for the BM-MSCs from WZSP, and Fascin was involved in regulating the migration capacity of the BM-MSCs in vitro, which may be useful for the BM-MSCs from WZSP to be utilized in regenerative therapy for human. https://epubs.icar.org.in/index.php/IJAnS/article/view/44250Bone marrow mesenchymal stem cellsFascinMigration capacitySpecific short hairpin RNAWuzhishan pig |
| spellingShingle | LING YANG YING XIA XIAO-LI TAO WEI HE TIAN-WEN WU LEI HUANG JIN-XUE RUAN SHU-LIN YANG YU-LIAN MU KUI LI SHU-TANG FENG Knockdown Fascin impairs bone marrow mesenchymal stem cells migration capacity of Wuzhishan Miniature pig Indian Journal of Animal Sciences Bone marrow mesenchymal stem cells Fascin Migration capacity Specific short hairpin RNA Wuzhishan pig |
| title | Knockdown Fascin impairs bone marrow mesenchymal stem cells migration capacity of Wuzhishan Miniature pig |
| title_full | Knockdown Fascin impairs bone marrow mesenchymal stem cells migration capacity of Wuzhishan Miniature pig |
| title_fullStr | Knockdown Fascin impairs bone marrow mesenchymal stem cells migration capacity of Wuzhishan Miniature pig |
| title_full_unstemmed | Knockdown Fascin impairs bone marrow mesenchymal stem cells migration capacity of Wuzhishan Miniature pig |
| title_short | Knockdown Fascin impairs bone marrow mesenchymal stem cells migration capacity of Wuzhishan Miniature pig |
| title_sort | knockdown fascin impairs bone marrow mesenchymal stem cells migration capacity of wuzhishan miniature pig |
| topic | Bone marrow mesenchymal stem cells Fascin Migration capacity Specific short hairpin RNA Wuzhishan pig |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/44250 |
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