Effects of noise exposure on catalase activity of growing lymphocytes
Oxidative stress due to noise was estimated at cell level using model of growing lymphocytes. Lymphocytes were isolated and cultured using conventional methodology. Cell culture of each group was exposed to sound of frequency 1 KHz during incubation. Three groups were defined on the basis of exposur...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2012-11-01
|
| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2550 |
| _version_ | 1797260889726910464 |
|---|---|
| author | Syed Kashif Nawaz Shahida Hasnain |
| author_facet | Syed Kashif Nawaz Shahida Hasnain |
| author_sort | Syed Kashif Nawaz |
| collection | DOAJ |
| description | Oxidative stress due to noise was estimated at cell level using model of growing lymphocytes. Lymphocytes were isolated and cultured using conventional methodology. Cell culture of each group was exposed to sound of frequency 1 KHz during incubation. Three groups were defined on the basis of exposure of sound with specific range of intensity and duration of exposure. Group A and Group B were exposed to sound with intensity 110 dBA for four hours per day and for eight hours per day respectively. Control group was exposed to sound less than 85 dBA. Viable cell count was performed using trypan blue. Catalase activity of each group was estimated using ELISA kit.
Viable cell count of Group A and Group B was almost same but significantly less than that of control group. Catalase activity of lymphocytes in Group B was significantly low as compared to Group A and controls (p=0.003,p< 0.05). There was no significant difference between catalase activity of Group A and control group.
Exposure of sound with frequency 1 KHz and intensity 110 dBA for 4 hours and eight hours per day may induce oxidative stress in growing lymphocytes causing the difference in viable cell count. However the catalase activity depends on duration of exposure. In case of noise exposure of 8 hours per day, it declines significantly as compared to noise exposure of 4 hours per day.
|
| first_indexed | 2024-04-24T23:32:30Z |
| format | Article |
| id | doaj.art-7b3a2c12e16c4f71918e5a5b0e89c4c6 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:30Z |
| publishDate | 2012-11-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-7b3a2c12e16c4f71918e5a5b0e89c4c62024-03-15T14:33:42ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2012-11-0111410.17305/bjbms.2011.2550323Effects of noise exposure on catalase activity of growing lymphocytesSyed Kashif Nawaz0Shahida Hasnain1Department of Microbiology and Molecular Genetics, University of the Punjab new CampusDepartment of Microbiology and Molecular Genetics, University of the Punjab new CampusOxidative stress due to noise was estimated at cell level using model of growing lymphocytes. Lymphocytes were isolated and cultured using conventional methodology. Cell culture of each group was exposed to sound of frequency 1 KHz during incubation. Three groups were defined on the basis of exposure of sound with specific range of intensity and duration of exposure. Group A and Group B were exposed to sound with intensity 110 dBA for four hours per day and for eight hours per day respectively. Control group was exposed to sound less than 85 dBA. Viable cell count was performed using trypan blue. Catalase activity of each group was estimated using ELISA kit. Viable cell count of Group A and Group B was almost same but significantly less than that of control group. Catalase activity of lymphocytes in Group B was significantly low as compared to Group A and controls (p=0.003,p< 0.05). There was no significant difference between catalase activity of Group A and control group. Exposure of sound with frequency 1 KHz and intensity 110 dBA for 4 hours and eight hours per day may induce oxidative stress in growing lymphocytes causing the difference in viable cell count. However the catalase activity depends on duration of exposure. In case of noise exposure of 8 hours per day, it declines significantly as compared to noise exposure of 4 hours per day. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2550Oxidative stresscatalase activitynoise exposureoxidative damage in lymphocytesnoise induced oxidative damage |
| spellingShingle | Syed Kashif Nawaz Shahida Hasnain Effects of noise exposure on catalase activity of growing lymphocytes Biomolecules & Biomedicine Oxidative stress catalase activity noise exposure oxidative damage in lymphocytes noise induced oxidative damage |
| title | Effects of noise exposure on catalase activity of growing lymphocytes |
| title_full | Effects of noise exposure on catalase activity of growing lymphocytes |
| title_fullStr | Effects of noise exposure on catalase activity of growing lymphocytes |
| title_full_unstemmed | Effects of noise exposure on catalase activity of growing lymphocytes |
| title_short | Effects of noise exposure on catalase activity of growing lymphocytes |
| title_sort | effects of noise exposure on catalase activity of growing lymphocytes |
| topic | Oxidative stress catalase activity noise exposure oxidative damage in lymphocytes noise induced oxidative damage |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2550 |
| work_keys_str_mv | AT syedkashifnawaz effectsofnoiseexposureoncatalaseactivityofgrowinglymphocytes AT shahidahasnain effectsofnoiseexposureoncatalaseactivityofgrowinglymphocytes |