The Multi-Omics Analysis Revealed a Metabolic Regulatory System of Cecum in Rabbit with Diarrhea
With the comprehensive prohibition of antibiotics in the feed industry in China, the incidence of diarrhea in rabbits increased, such as loss of appetite, vomiting, and excretion of atheromatous feces. In order to explore the pathological and the molecular mechanisms of the diarrhea in the rabbitry...
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MDPI AG
2022-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/9/1194 |
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| author | Jie Wang Kaisen Zhao Zhe Kang Meigui Wang Yang Chen Huimei Fan Siqi Xia Songjia Lai |
| author_facet | Jie Wang Kaisen Zhao Zhe Kang Meigui Wang Yang Chen Huimei Fan Siqi Xia Songjia Lai |
| author_sort | Jie Wang |
| collection | DOAJ |
| description | With the comprehensive prohibition of antibiotics in the feed industry in China, the incidence of diarrhea in rabbits increased, such as loss of appetite, vomiting, and excretion of atheromatous feces. In order to explore the pathological and the molecular mechanisms of the diarrhea in the rabbitry fed with antibiotic-free diet, we used microbial metagenomics, transcriptome, and non-targeted metabolomics sequencing. The results showed that the Firmicutes level was significantly decreased (<i>p</i> < 0.001) and the Proteobacteria level was significantly increased (<i>p</i> < 0.05). The functional enrichment of cecum revealed that most differentially expressed genes (DEGs) were expressed in immune, inflammatory, and metabolic processes. The enrichment of the cecal fecal metabolites focused on the bile secretion, antifolate resistance, and tryptophan metabolism pathways, which are mainly associated with inflammation. The results of correlation analysis showed that Fournierella was positively correlated with myricetin, ursolic acid, and furtherly might cause bile secretion and tryptophan metabolism disorder, aggravate intestinal inflammation, change intestinal permeability, and reduce host immunity, leading to diarrhea in rabbits. This study provides a theoretical basis for illustrating the reason for diarrhea and developing new feeds for the health of rabbits. |
| first_indexed | 2024-03-10T04:23:57Z |
| format | Article |
| id | doaj.art-7b3b965ac38c400080acab8297116002 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T04:23:57Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-7b3b965ac38c400080acab82971160022023-11-23T07:43:39ZengMDPI AGAnimals2076-26152022-05-01129119410.3390/ani12091194The Multi-Omics Analysis Revealed a Metabolic Regulatory System of Cecum in Rabbit with DiarrheaJie Wang0Kaisen Zhao1Zhe Kang2Meigui Wang3Yang Chen4Huimei Fan5Siqi Xia6Songjia Lai7College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu Campus, Sichuan Agricultural University, Chengdu 611130, ChinaWith the comprehensive prohibition of antibiotics in the feed industry in China, the incidence of diarrhea in rabbits increased, such as loss of appetite, vomiting, and excretion of atheromatous feces. In order to explore the pathological and the molecular mechanisms of the diarrhea in the rabbitry fed with antibiotic-free diet, we used microbial metagenomics, transcriptome, and non-targeted metabolomics sequencing. The results showed that the Firmicutes level was significantly decreased (<i>p</i> < 0.001) and the Proteobacteria level was significantly increased (<i>p</i> < 0.05). The functional enrichment of cecum revealed that most differentially expressed genes (DEGs) were expressed in immune, inflammatory, and metabolic processes. The enrichment of the cecal fecal metabolites focused on the bile secretion, antifolate resistance, and tryptophan metabolism pathways, which are mainly associated with inflammation. The results of correlation analysis showed that Fournierella was positively correlated with myricetin, ursolic acid, and furtherly might cause bile secretion and tryptophan metabolism disorder, aggravate intestinal inflammation, change intestinal permeability, and reduce host immunity, leading to diarrhea in rabbits. This study provides a theoretical basis for illustrating the reason for diarrhea and developing new feeds for the health of rabbits.https://www.mdpi.com/2076-2615/12/9/1194rabbitcecumdiarrheamicroorganismtranscriptomeuntargeted metabolomics |
| spellingShingle | Jie Wang Kaisen Zhao Zhe Kang Meigui Wang Yang Chen Huimei Fan Siqi Xia Songjia Lai The Multi-Omics Analysis Revealed a Metabolic Regulatory System of Cecum in Rabbit with Diarrhea Animals rabbit cecum diarrhea microorganism transcriptome untargeted metabolomics |
| title | The Multi-Omics Analysis Revealed a Metabolic Regulatory System of Cecum in Rabbit with Diarrhea |
| title_full | The Multi-Omics Analysis Revealed a Metabolic Regulatory System of Cecum in Rabbit with Diarrhea |
| title_fullStr | The Multi-Omics Analysis Revealed a Metabolic Regulatory System of Cecum in Rabbit with Diarrhea |
| title_full_unstemmed | The Multi-Omics Analysis Revealed a Metabolic Regulatory System of Cecum in Rabbit with Diarrhea |
| title_short | The Multi-Omics Analysis Revealed a Metabolic Regulatory System of Cecum in Rabbit with Diarrhea |
| title_sort | multi omics analysis revealed a metabolic regulatory system of cecum in rabbit with diarrhea |
| topic | rabbit cecum diarrhea microorganism transcriptome untargeted metabolomics |
| url | https://www.mdpi.com/2076-2615/12/9/1194 |
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