Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical Biosensor
The aim of this study is to covalently immobilize horse radish peroxidase (HRP) onto new hybrid membranes synthesized by the sol-gel method based on silica precursors, dendrimers and cellulose derivatives. This new system will be used for designing biosensor. For investigation of the properties of m...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bulgarian Academy of Sciences
2015-06-01
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| Series: | International Journal Bioautomation |
| Subjects: | |
| Online Access: | http://www.biomed.bas.bg/bioautomation/2015/vol_19.2/files/19.2_05.pdf |
| _version_ | 1828826028948062208 |
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| author | Lyubov Yotova Ahmed Hassaan Spaska Yaneva |
| author_facet | Lyubov Yotova Ahmed Hassaan Spaska Yaneva |
| author_sort | Lyubov Yotova |
| collection | DOAJ |
| description | The aim of this study is to covalently immobilize horse radish peroxidase (HRP) onto new hybrid membranes synthesized by the sol-gel method based on silica precursors, dendrimers and cellulose derivatives. This new system will be used for designing biosensor. For investigation of the properties of membranes, HRP was used as a modeling enzyme. Kinetic parameters, pH and temperature optimum were determined, and the structure of the membranes surface was examined. Results showed higher relative and residual activity of HRP immobilized onto membranes with cellulose acetate butyrate with high molecular weight CAB/H. This novel biosensor could offer a simple, cheap and rapid tool with enhanced sensing performance as well as having potentials to find application in medicine, pharmacy, food and process control and environmental monitoring. |
| first_indexed | 2024-12-12T14:30:20Z |
| format | Article |
| id | doaj.art-7b3bd5f4d95245d6a75e06b59a714a8b |
| institution | Directory Open Access Journal |
| issn | 1314-1902 1314-2321 |
| language | English |
| last_indexed | 2024-12-12T14:30:20Z |
| publishDate | 2015-06-01 |
| publisher | Bulgarian Academy of Sciences |
| record_format | Article |
| series | International Journal Bioautomation |
| spelling | doaj.art-7b3bd5f4d95245d6a75e06b59a714a8b2022-12-22T00:21:33ZengBulgarian Academy of SciencesInternational Journal Bioautomation1314-19021314-23212015-06-01192177186Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical BiosensorLyubov Yotova0Ahmed HassaanSpaska YanevaDepartment of Biotechnology, University of Chemical Technology and Metallurgy, 8 Kl. Okhridski Blvd, 1756 Sofia, BulgariaThe aim of this study is to covalently immobilize horse radish peroxidase (HRP) onto new hybrid membranes synthesized by the sol-gel method based on silica precursors, dendrimers and cellulose derivatives. This new system will be used for designing biosensor. For investigation of the properties of membranes, HRP was used as a modeling enzyme. Kinetic parameters, pH and temperature optimum were determined, and the structure of the membranes surface was examined. Results showed higher relative and residual activity of HRP immobilized onto membranes with cellulose acetate butyrate with high molecular weight CAB/H. This novel biosensor could offer a simple, cheap and rapid tool with enhanced sensing performance as well as having potentials to find application in medicine, pharmacy, food and process control and environmental monitoring.http://www.biomed.bas.bg/bioautomation/2015/vol_19.2/files/19.2_05.pdfEnzymesSol-gelMycotoxins |
| spellingShingle | Lyubov Yotova Ahmed Hassaan Spaska Yaneva Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical Biosensor International Journal Bioautomation Enzymes Sol-gel Mycotoxins |
| title | Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical Biosensor |
| title_full | Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical Biosensor |
| title_fullStr | Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical Biosensor |
| title_full_unstemmed | Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical Biosensor |
| title_short | Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical Biosensor |
| title_sort | covalent immobilization of peroxidase onto hybrid membranes for the construction of optical biosensor |
| topic | Enzymes Sol-gel Mycotoxins |
| url | http://www.biomed.bas.bg/bioautomation/2015/vol_19.2/files/19.2_05.pdf |
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