Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses
Abstract Background Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-a...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13048-022-01064-3 |
| _version_ | 1811188217180848128 |
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| author | Julie Barberet Bastien Ducreux Céline Bruno Magali Guilleman Raymond Simonot Nicolas Lieury Adrien Guilloteau Déborah Bourc’his Patricia Fauque |
| author_facet | Julie Barberet Bastien Ducreux Céline Bruno Magali Guilleman Raymond Simonot Nicolas Lieury Adrien Guilloteau Déborah Bourc’his Patricia Fauque |
| author_sort | Julie Barberet |
| collection | DOAJ |
| description | Abstract Background Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to empty micro-injection gesture as compared with a manual method. Additionally, we intended to evaluate transcriptomic consequences of both techniques using single-cell RNA-seq technology. Results Post-warming survival rate, oocyte surfaces and resistance to empty micro-injection were comparable between semi-automated and manual vitrification groups. Both oocyte vitrification techniques showed limited differences in the resulting transcriptomic profile of sibling oocytes since only 5 differentially expressed genes were identified. Additionally, there was no difference in median transcript integrity number or percentage of mitochondrial DNA between the two groups. However, a total of 108 genes were differentially expressed between fresh and vitrified oocytes (FDR < 0.05) and showed over-represented of genes related to important cellular process. Conclusions Our results provide reassurance about the influence of semi-automation as compared with the manual vitrification method. Concerning oocyte vitrification itself, no tight common transcriptomic signature associated has been observed across studies. Trial registration NCT03570073. |
| first_indexed | 2024-04-11T14:15:55Z |
| format | Article |
| id | doaj.art-7b3fa67389d84e6b88f5f75c23e624fc |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T14:15:55Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-7b3fa67389d84e6b88f5f75c23e624fc2022-12-22T04:19:25ZengBMCJournal of Ovarian Research1757-22152022-12-0115111410.1186/s13048-022-01064-3Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analysesJulie Barberet0Bastien Ducreux1Céline Bruno2Magali Guilleman3Raymond Simonot4Nicolas Lieury5Adrien Guilloteau6Déborah Bourc’his7Patricia Fauque8Université Bourgogne Franche-Comté - Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231Université Bourgogne Franche-Comté - Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231Université Bourgogne Franche-Comté - Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231Université Bourgogne Franche-Comté - Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231CHU Dijon Bourgogne, Laboratoire de Biologie de la Reproduction – CECOSCHU Dijon Bourgogne, Laboratoire de Biologie de la Reproduction – CECOSUSMR, Dijon Bourgogne University HospitalInstitut Curie, PSL University, CNRS, INSERMUniversité Bourgogne Franche-Comté - Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231Abstract Background Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to empty micro-injection gesture as compared with a manual method. Additionally, we intended to evaluate transcriptomic consequences of both techniques using single-cell RNA-seq technology. Results Post-warming survival rate, oocyte surfaces and resistance to empty micro-injection were comparable between semi-automated and manual vitrification groups. Both oocyte vitrification techniques showed limited differences in the resulting transcriptomic profile of sibling oocytes since only 5 differentially expressed genes were identified. Additionally, there was no difference in median transcript integrity number or percentage of mitochondrial DNA between the two groups. However, a total of 108 genes were differentially expressed between fresh and vitrified oocytes (FDR < 0.05) and showed over-represented of genes related to important cellular process. Conclusions Our results provide reassurance about the influence of semi-automation as compared with the manual vitrification method. Concerning oocyte vitrification itself, no tight common transcriptomic signature associated has been observed across studies. Trial registration NCT03570073.https://doi.org/10.1186/s13048-022-01064-3Assisted reproductive technologyCryopreservationOocyteSingle-cell RNA-seqVitrification |
| spellingShingle | Julie Barberet Bastien Ducreux Céline Bruno Magali Guilleman Raymond Simonot Nicolas Lieury Adrien Guilloteau Déborah Bourc’his Patricia Fauque Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses Journal of Ovarian Research Assisted reproductive technology Cryopreservation Oocyte Single-cell RNA-seq Vitrification |
| title | Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses |
| title_full | Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses |
| title_fullStr | Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses |
| title_full_unstemmed | Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses |
| title_short | Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses |
| title_sort | comparison of oocyte vitrification using a semi automated or a manual closed system in human siblings survival and transcriptomic analyses |
| topic | Assisted reproductive technology Cryopreservation Oocyte Single-cell RNA-seq Vitrification |
| url | https://doi.org/10.1186/s13048-022-01064-3 |
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