Analysis of the response regulatory network of pepper genes under hydrogen peroxide stress
Hydrogen peroxide (H2O2) is a regulatory component related to plant signal transduction. To better understand the genome-wide gene expression response to H2O2 stress in pepper plants, a regulatory network of H2O2 stress-gene expression in pepper leaves and roots was constructed in the present study....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1018991/full |
| _version_ | 1811185157430837248 |
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| author | Bingqian Tang Bingqian Tang Bingqian Tang Guangbin Yang Juan Du Lingling Xie Jin Wang Luzhao Pan Yin Luo Qingyun Shan Xuexiao Zou Xuexiao Zou Xuexiao Zou Xuexiao Zou Cheng Xiong Cheng Xiong Feng Liu Feng Liu Feng Liu Feng Liu |
| author_facet | Bingqian Tang Bingqian Tang Bingqian Tang Guangbin Yang Juan Du Lingling Xie Jin Wang Luzhao Pan Yin Luo Qingyun Shan Xuexiao Zou Xuexiao Zou Xuexiao Zou Xuexiao Zou Cheng Xiong Cheng Xiong Feng Liu Feng Liu Feng Liu Feng Liu |
| author_sort | Bingqian Tang |
| collection | DOAJ |
| description | Hydrogen peroxide (H2O2) is a regulatory component related to plant signal transduction. To better understand the genome-wide gene expression response to H2O2 stress in pepper plants, a regulatory network of H2O2 stress-gene expression in pepper leaves and roots was constructed in the present study. We collected the normal tissues of leaves and roots of pepper plants after 40 days of H2O2 treatment and obtained the RNA-seq data of leaves and roots exposed to H2O2 for 0.5–24 h. By comparing the gene responses of pepper leaves and roots exposed to H2O2 stress for different time periods, we found that the response in roots reached the peak at 3 h, whereas the response in leaves reached the peak at 24 h after treatment, and the response degree in the roots was higher than that in the leaves. We used all datasets for K-means analysis and network analysis identified the clusters related to stress response and related genes. In addition, CaEBS1, CaRAP2, and CabHLH029 were identified through a co-expression analysis and were found to be strongly related to several reactive oxygen species-scavenging enzyme genes; their homologous genes in Arabidopsis showed important functions in response to hypoxia or iron uptake. This study provides a theoretical basis for determining the dynamic response process of pepper plants to H2O2 stress in leaves and roots, as well as for determining the critical time and the molecular mechanism of H2O2 stress response in leaves and roots. The candidate transcription factors identified in this study can be used as a reference for further experimental verification. |
| first_indexed | 2024-04-11T13:24:49Z |
| format | Article |
| id | doaj.art-7b4089b6f6ba472c8748bc33c7df12a9 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T13:24:49Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7b4089b6f6ba472c8748bc33c7df12a92022-12-22T04:22:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10189911018991Analysis of the response regulatory network of pepper genes under hydrogen peroxide stressBingqian Tang0Bingqian Tang1Bingqian Tang2Guangbin Yang3Juan Du4Lingling Xie5Jin Wang6Luzhao Pan7Yin Luo8Qingyun Shan9Xuexiao Zou10Xuexiao Zou11Xuexiao Zou12Xuexiao Zou13Cheng Xiong14Cheng Xiong15Feng Liu16Feng Liu17Feng Liu18Feng Liu19College of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaLongping Branch, Graduate School of Hunan University, Changsha, Hunan, ChinaEngineering Research Center for Germplasm Innovation and New Varieties Breeding of Horticultural Crops, Key Laboratory for Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, ChinaLongping Branch, Graduate School of Hunan University, Changsha, Hunan, ChinaLongping Branch, Graduate School of Hunan University, Changsha, Hunan, ChinaEngineering Research Center for Germplasm Innovation and New Varieties Breeding of Horticultural Crops, Key Laboratory for Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaLongping Branch, Graduate School of Hunan University, Changsha, Hunan, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaLongping Branch, Graduate School of Hunan University, Changsha, Hunan, ChinaEngineering Research Center for Germplasm Innovation and New Varieties Breeding of Horticultural Crops, Key Laboratory for Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, ChinaLaboratory of Lingnan Modern Agriculture, Guangzhou, Guangdong, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaEngineering Research Center for Germplasm Innovation and New Varieties Breeding of Horticultural Crops, Key Laboratory for Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan, ChinaLongping Branch, Graduate School of Hunan University, Changsha, Hunan, ChinaEngineering Research Center for Germplasm Innovation and New Varieties Breeding of Horticultural Crops, Key Laboratory for Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, ChinaLaboratory of Lingnan Modern Agriculture, Guangzhou, Guangdong, ChinaHydrogen peroxide (H2O2) is a regulatory component related to plant signal transduction. To better understand the genome-wide gene expression response to H2O2 stress in pepper plants, a regulatory network of H2O2 stress-gene expression in pepper leaves and roots was constructed in the present study. We collected the normal tissues of leaves and roots of pepper plants after 40 days of H2O2 treatment and obtained the RNA-seq data of leaves and roots exposed to H2O2 for 0.5–24 h. By comparing the gene responses of pepper leaves and roots exposed to H2O2 stress for different time periods, we found that the response in roots reached the peak at 3 h, whereas the response in leaves reached the peak at 24 h after treatment, and the response degree in the roots was higher than that in the leaves. We used all datasets for K-means analysis and network analysis identified the clusters related to stress response and related genes. In addition, CaEBS1, CaRAP2, and CabHLH029 were identified through a co-expression analysis and were found to be strongly related to several reactive oxygen species-scavenging enzyme genes; their homologous genes in Arabidopsis showed important functions in response to hypoxia or iron uptake. This study provides a theoretical basis for determining the dynamic response process of pepper plants to H2O2 stress in leaves and roots, as well as for determining the critical time and the molecular mechanism of H2O2 stress response in leaves and roots. The candidate transcription factors identified in this study can be used as a reference for further experimental verification.https://www.frontiersin.org/articles/10.3389/fpls.2022.1018991/fullhydrogen peroxide stressreactive oxygen speciespeppergene regulatory networktissue specificity |
| spellingShingle | Bingqian Tang Bingqian Tang Bingqian Tang Guangbin Yang Juan Du Lingling Xie Jin Wang Luzhao Pan Yin Luo Qingyun Shan Xuexiao Zou Xuexiao Zou Xuexiao Zou Xuexiao Zou Cheng Xiong Cheng Xiong Feng Liu Feng Liu Feng Liu Feng Liu Analysis of the response regulatory network of pepper genes under hydrogen peroxide stress Frontiers in Plant Science hydrogen peroxide stress reactive oxygen species pepper gene regulatory network tissue specificity |
| title | Analysis of the response regulatory network of pepper genes under hydrogen peroxide stress |
| title_full | Analysis of the response regulatory network of pepper genes under hydrogen peroxide stress |
| title_fullStr | Analysis of the response regulatory network of pepper genes under hydrogen peroxide stress |
| title_full_unstemmed | Analysis of the response regulatory network of pepper genes under hydrogen peroxide stress |
| title_short | Analysis of the response regulatory network of pepper genes under hydrogen peroxide stress |
| title_sort | analysis of the response regulatory network of pepper genes under hydrogen peroxide stress |
| topic | hydrogen peroxide stress reactive oxygen species pepper gene regulatory network tissue specificity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1018991/full |
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