Macrophage-like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study
With the introduction of a new interdisciplinary field, osteoimmunology, today, it is well acknowledged that biomaterial-induced inflammation is modulated by immune cells, primarily macrophages, and can be controlled by nanotopographical cues. Recent studies have investigated the effect of surface p...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/7/3558 |
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| author | Madalina Georgiana Necula Anca Mazare Andreea Mariana Negrescu Valentina Mitran Selda Ozkan Roxana Trusca Jung Park Patrik Schmuki Anisoara Cimpean |
| author_facet | Madalina Georgiana Necula Anca Mazare Andreea Mariana Negrescu Valentina Mitran Selda Ozkan Roxana Trusca Jung Park Patrik Schmuki Anisoara Cimpean |
| author_sort | Madalina Georgiana Necula |
| collection | DOAJ |
| description | With the introduction of a new interdisciplinary field, osteoimmunology, today, it is well acknowledged that biomaterial-induced inflammation is modulated by immune cells, primarily macrophages, and can be controlled by nanotopographical cues. Recent studies have investigated the effect of surface properties in modulating the immune reaction, and literature data indicate that various surface cues can dictate both the immune response and bone tissue repair. In this context, the purpose of the present study was to investigate the effects of titanium dioxide nanotube (TNT) interspacing on the response of the macrophage-like cell line RAW 264.7. The cells were maintained in contact with the surfaces of flat titanium (Ti) and anodic TNTs with an intertube spacing of 20 nm (TNT20) and 80 nm (TNT80), under standard or pro-inflammatory conditions. The results revealed that nanotube interspacing can influence macrophage response in terms of cell survival and proliferation, cellular morphology and polarization, cytokine/chemokine expression, and foreign body reaction. While the nanostructured topography did not tune the macrophages’ differentiation into osteoclasts, this behavior was significantly reduced as compared to flat Ti surface. Overall, this study provides a new insight into how nanotubes’ morphological features, particularly intertube spacing, could affect macrophage behavior. |
| first_indexed | 2024-03-09T11:47:37Z |
| format | Article |
| id | doaj.art-7b43985c4b364b419689854c49a9cbde |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T11:47:37Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7b43985c4b364b419689854c49a9cbde2023-11-30T23:18:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01237355810.3390/ijms23073558Macrophage-like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro StudyMadalina Georgiana Necula0Anca Mazare1Andreea Mariana Negrescu2Valentina Mitran3Selda Ozkan4Roxana Trusca5Jung Park6Patrik Schmuki7Anisoara Cimpean8Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaDepartment of Materials Science WW4-LKO, Friedrich-Alexander University, 91058 Erlangen, GermanyDepartment of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaDepartment of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaDepartment of Materials Science WW4-LKO, Friedrich-Alexander University, 91058 Erlangen, GermanyFaculty of Engineering in Foreign Languages, University Politehnica of Bucharest, 313 Splaiul Indendentei, 060042 Bucharest, RomaniaDepartment of Pediatrics, Division of Molecular Pediatrics, University Hospital Erlangen, 91054 Erlangen, GermanyDepartment of Materials Science WW4-LKO, Friedrich-Alexander University, 91058 Erlangen, GermanyDepartment of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaWith the introduction of a new interdisciplinary field, osteoimmunology, today, it is well acknowledged that biomaterial-induced inflammation is modulated by immune cells, primarily macrophages, and can be controlled by nanotopographical cues. Recent studies have investigated the effect of surface properties in modulating the immune reaction, and literature data indicate that various surface cues can dictate both the immune response and bone tissue repair. In this context, the purpose of the present study was to investigate the effects of titanium dioxide nanotube (TNT) interspacing on the response of the macrophage-like cell line RAW 264.7. The cells were maintained in contact with the surfaces of flat titanium (Ti) and anodic TNTs with an intertube spacing of 20 nm (TNT20) and 80 nm (TNT80), under standard or pro-inflammatory conditions. The results revealed that nanotube interspacing can influence macrophage response in terms of cell survival and proliferation, cellular morphology and polarization, cytokine/chemokine expression, and foreign body reaction. While the nanostructured topography did not tune the macrophages’ differentiation into osteoclasts, this behavior was significantly reduced as compared to flat Ti surface. Overall, this study provides a new insight into how nanotubes’ morphological features, particularly intertube spacing, could affect macrophage behavior.https://www.mdpi.com/1422-0067/23/7/3558macrophageinflammationTiO<sub>2</sub> nanotubesintertube spacingcytokinesosteoclastogenesis |
| spellingShingle | Madalina Georgiana Necula Anca Mazare Andreea Mariana Negrescu Valentina Mitran Selda Ozkan Roxana Trusca Jung Park Patrik Schmuki Anisoara Cimpean Macrophage-like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study International Journal of Molecular Sciences macrophage inflammation TiO<sub>2</sub> nanotubes intertube spacing cytokines osteoclastogenesis |
| title | Macrophage-like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study |
| title_full | Macrophage-like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study |
| title_fullStr | Macrophage-like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study |
| title_full_unstemmed | Macrophage-like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study |
| title_short | Macrophage-like Cells Are Responsive to Titania Nanotube Intertube Spacing—An In Vitro Study |
| title_sort | macrophage like cells are responsive to titania nanotube intertube spacing an in vitro study |
| topic | macrophage inflammation TiO<sub>2</sub> nanotubes intertube spacing cytokines osteoclastogenesis |
| url | https://www.mdpi.com/1422-0067/23/7/3558 |
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