Prevalence and Antibiogram of <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique
<i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> are major public health problems and the main cause of bacterial disease in Nile tilapia (<i>Oreochromis niloticus</i>). This study was conducted to determine the prevalence, antibiotic resistance and...
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MDPI AG
2021-05-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/6/654 |
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| author | Hanan A. Zaher Mohamad I. Nofal Basma M. Hendam Moustafa M. Elshaer Abdulaziz S. Alothaim Mostafa M. Eraqi |
| author_facet | Hanan A. Zaher Mohamad I. Nofal Basma M. Hendam Moustafa M. Elshaer Abdulaziz S. Alothaim Mostafa M. Eraqi |
| author_sort | Hanan A. Zaher |
| collection | DOAJ |
| description | <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> are major public health problems and the main cause of bacterial disease in Nile tilapia (<i>Oreochromis niloticus</i>). This study was conducted to determine the prevalence, antibiotic resistance and some virulence genes of both <i>V. parahaemolyticus</i> and <i>A. hydrophila</i> isolates from Nile tilapia. From Manzala Farm at Dakahlia governorate, 250 freshwater fish samples were collected. The confirmed bacterial isolates from the examined Nile tilapia samples in the study were 24.8% (62/250) for <i>V. parahaemolyticus</i> and 19.2% (48/250) for <i>A. hydrophila</i>. multiplex PCR, revealing that the <i>tlh</i> gene was found in 46.7% (29/62) of <i>V. parahaemolyticus</i> isolates, while the <i>tdh</i> and <i>trh</i> virulence genes were found in 17.2% (5/29). Meanwhile, 39.5% (19/48) of <i>A. hydrophila</i> isolates had the <i>16s rRNA</i> gene and 10.5% (2/19) had the <i>aerA</i> and <i>ahh1</i> virulence genes. The Multiple Antibiotic Resistance indices of <i>V. parahaemolyticus</i> and <i>A. hydrophila</i> were 0.587 and 0.586, respectively. In conclusion, alternative non-antibiotic control strategies for bacterial infections in farmed fish should be promoted to avoid multidrug-resistant bacteria. Therefore, it is suggested that farmers should be skilled in basic fish health control and that molecular detection methods are more rapid and cost-effective than bacteriological methods. |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T10:52:43Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj.art-7b44757216484b8abb0003b76cadf0b22023-11-21T22:06:10ZengMDPI AGAntibiotics2079-63822021-05-0110665410.3390/antibiotics10060654Prevalence and Antibiogram of <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR TechniqueHanan A. Zaher0Mohamad I. Nofal1Basma M. Hendam2Moustafa M. Elshaer3Abdulaziz S. Alothaim4Mostafa M. Eraqi5Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptGeneral Authority of Fish Resources and Development (GAFRD), Manzala Fish Farm, Manzala 35642, EgyptDepartment of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Microbiology at Specialized Medical Hospital, Mansoura University, Mansoura 35516, EgyptDepartment of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi ArabiaDepartment of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi Arabia<i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> are major public health problems and the main cause of bacterial disease in Nile tilapia (<i>Oreochromis niloticus</i>). This study was conducted to determine the prevalence, antibiotic resistance and some virulence genes of both <i>V. parahaemolyticus</i> and <i>A. hydrophila</i> isolates from Nile tilapia. From Manzala Farm at Dakahlia governorate, 250 freshwater fish samples were collected. The confirmed bacterial isolates from the examined Nile tilapia samples in the study were 24.8% (62/250) for <i>V. parahaemolyticus</i> and 19.2% (48/250) for <i>A. hydrophila</i>. multiplex PCR, revealing that the <i>tlh</i> gene was found in 46.7% (29/62) of <i>V. parahaemolyticus</i> isolates, while the <i>tdh</i> and <i>trh</i> virulence genes were found in 17.2% (5/29). Meanwhile, 39.5% (19/48) of <i>A. hydrophila</i> isolates had the <i>16s rRNA</i> gene and 10.5% (2/19) had the <i>aerA</i> and <i>ahh1</i> virulence genes. The Multiple Antibiotic Resistance indices of <i>V. parahaemolyticus</i> and <i>A. hydrophila</i> were 0.587 and 0.586, respectively. In conclusion, alternative non-antibiotic control strategies for bacterial infections in farmed fish should be promoted to avoid multidrug-resistant bacteria. Therefore, it is suggested that farmers should be skilled in basic fish health control and that molecular detection methods are more rapid and cost-effective than bacteriological methods.https://www.mdpi.com/2079-6382/10/6/654molecular identification<i>Aeromonas hydrophila</i><i>Vibrio parahaemolyticus</i><i>Oreochromis niloticus</i> |
| spellingShingle | Hanan A. Zaher Mohamad I. Nofal Basma M. Hendam Moustafa M. Elshaer Abdulaziz S. Alothaim Mostafa M. Eraqi Prevalence and Antibiogram of <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique Antibiotics molecular identification <i>Aeromonas hydrophila</i> <i>Vibrio parahaemolyticus</i> <i>Oreochromis niloticus</i> |
| title | Prevalence and Antibiogram of <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique |
| title_full | Prevalence and Antibiogram of <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique |
| title_fullStr | Prevalence and Antibiogram of <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique |
| title_full_unstemmed | Prevalence and Antibiogram of <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique |
| title_short | Prevalence and Antibiogram of <i>Vibrio parahaemolyticus</i> and <i>Aeromonas hydrophila</i> in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique |
| title_sort | prevalence and antibiogram of i vibrio parahaemolyticus i and i aeromonas hydrophila i in the flesh of nile tilapia with special reference to their virulence genes detected using multiplex pcr technique |
| topic | molecular identification <i>Aeromonas hydrophila</i> <i>Vibrio parahaemolyticus</i> <i>Oreochromis niloticus</i> |
| url | https://www.mdpi.com/2079-6382/10/6/654 |
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