Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats
Introduction: Angiogenesis, the formation of new capillaries from pre-existing vessels, crucially involves activation of the hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF) genes. This study investigates the impact of exercise intensity on the expression of angiogeni...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Ilam University of Medical Sciences
2023-12-01
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| Series: | Journal of Basic Research in Medical Sciences |
| Subjects: | |
| Online Access: | http://jbrms.medilam.ac.ir/article-1-495-en.pdf |
| _version_ | 1797361153199833088 |
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| author | Shokoufeh Kheradmand Mohammad Reza Asad , Reza Mir Javadi Narges Kheradmand Mohamad Fashi |
| author_facet | Shokoufeh Kheradmand Mohammad Reza Asad , Reza Mir Javadi Narges Kheradmand Mohamad Fashi |
| author_sort | Shokoufeh Kheradmand |
| collection | DOAJ |
| description | Introduction: Angiogenesis, the formation of new capillaries from pre-existing vessels, crucially involves activation of the hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF) genes. This study investigates the impact of exercise intensity on the expression of angiogenic genes in the hearts of male rats.
Material & Methods: Eighteen male Wistar rats were randomly assigned to three groups: High-Intensity Interval Training (HIIT), Continuous Training (CT), and control (C). Both HIIT and CT groups underwent 8 weeks of training with five sessions per week. Anesthesia and blood sampling occurred 48 hours post final training session. Gene levels of HIF-1 and VEGF were measured in the left ventricle. Data analysis employed ANOVA and LSD post hoc tests (P≤0.05).
Results: VEGF gene expression significantly increased in both HIIT and CT groups compared to the control group (P = 0.001), with a more pronounced elevation in the HIIT group than the CT group (P = 0.004). Furthermore, HIF-1 levels exhibited a significant reduction in both HIIT (P = 0.001) and CT (P = 0.001) groups compared to the control group, with the HIIT-induced decrease surpassing that of the CT group (P = 0.049).
Conclusion: The noteworthy elevation in VEGF and decrease in HIF-1 gene expression levels in trained rats imply that exercise training enhances angiogenesis. Importantly, the extent of this enhancement is contingent upon exercise intensity, with HIIT demonstrating more pronounced positive effects on VEGF levels. |
| first_indexed | 2024-03-08T15:49:51Z |
| format | Article |
| id | doaj.art-7b46c62e8ded4d808936495da92af5e5 |
| institution | Directory Open Access Journal |
| issn | 2383-0506 2383-0972 |
| language | English |
| last_indexed | 2024-03-08T15:49:51Z |
| publishDate | 2023-12-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Journal of Basic Research in Medical Sciences |
| spelling | doaj.art-7b46c62e8ded4d808936495da92af5e52024-01-09T07:06:11ZengIlam University of Medical SciencesJournal of Basic Research in Medical Sciences2383-05062383-09722023-12-011044353Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male RatsShokoufeh Kheradmand0Mohammad Reza Asad1, Reza Mir Javadi2Narges Kheradmand3Mohamad Fashi4 Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran Department of Physical Education and Sport Sciences, Payame Noor University of Karaj, Alborz, Iran Department of Sport Physiology, Karaj Branch, Islamic Azad University, Karaj, Iran Department of Physical Education and Sport Sciences, Payame Noor University, Garmsar, Iran Department of Exercise Physiology, Faculty of Physical Education and Health Sciences, Shahid- Beheshti University, Tehran, Iran Introduction: Angiogenesis, the formation of new capillaries from pre-existing vessels, crucially involves activation of the hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF) genes. This study investigates the impact of exercise intensity on the expression of angiogenic genes in the hearts of male rats. Material & Methods: Eighteen male Wistar rats were randomly assigned to three groups: High-Intensity Interval Training (HIIT), Continuous Training (CT), and control (C). Both HIIT and CT groups underwent 8 weeks of training with five sessions per week. Anesthesia and blood sampling occurred 48 hours post final training session. Gene levels of HIF-1 and VEGF were measured in the left ventricle. Data analysis employed ANOVA and LSD post hoc tests (P≤0.05). Results: VEGF gene expression significantly increased in both HIIT and CT groups compared to the control group (P = 0.001), with a more pronounced elevation in the HIIT group than the CT group (P = 0.004). Furthermore, HIF-1 levels exhibited a significant reduction in both HIIT (P = 0.001) and CT (P = 0.001) groups compared to the control group, with the HIIT-induced decrease surpassing that of the CT group (P = 0.049). Conclusion: The noteworthy elevation in VEGF and decrease in HIF-1 gene expression levels in trained rats imply that exercise training enhances angiogenesis. Importantly, the extent of this enhancement is contingent upon exercise intensity, with HIIT demonstrating more pronounced positive effects on VEGF levels.http://jbrms.medilam.ac.ir/article-1-495-en.pdfexercise intensitycontinuous traininghigh-intensity interval trainingheart tissue |
| spellingShingle | Shokoufeh Kheradmand Mohammad Reza Asad , Reza Mir Javadi Narges Kheradmand Mohamad Fashi Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats Journal of Basic Research in Medical Sciences exercise intensity continuous training high-intensity interval training heart tissue |
| title | Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats |
| title_full | Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats |
| title_fullStr | Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats |
| title_full_unstemmed | Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats |
| title_short | Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats |
| title_sort | influence of exercise intensity on the expression of angiogenesis related genes in the hearts of male rats |
| topic | exercise intensity continuous training high-intensity interval training heart tissue |
| url | http://jbrms.medilam.ac.ir/article-1-495-en.pdf |
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