Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targets
Abstract Background A need for improved, cassette-based automation of 61Cu separation from irradiated Ni targets was identified given the growing interest in theranostics, and generally lengthy separation chemistries for 64Cu/64Ni, upon which 61Cu chemistry is often based. Methods A method for separ...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-11-01
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| Series: | EJNMMI Radiopharmacy and Chemistry |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41181-020-00108-7 |
| _version_ | 1828467012352868352 |
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| author | Johan Svedjehed Christopher J. Kutyreff Jonathan W. Engle Katherine Gagnon |
| author_facet | Johan Svedjehed Christopher J. Kutyreff Jonathan W. Engle Katherine Gagnon |
| author_sort | Johan Svedjehed |
| collection | DOAJ |
| description | Abstract Background A need for improved, cassette-based automation of 61Cu separation from irradiated Ni targets was identified given the growing interest in theranostics, and generally lengthy separation chemistries for 64Cu/64Ni, upon which 61Cu chemistry is often based. Methods A method for separating 61Cu from irradiated natNi targets was therefore developed, with provision for target recycling. Following deuteron irradiation, electroplated natNi targets were remotely transferred from the cyclotron and dissolved in acid. The dissolved target solution was then transferred to an automated FASTlab chemistry module, where sequential TBP and TK201 (Triskem) resins isolated the [61Cu]CuCl2, removed Ni, Co, and Fe, and concentrated the product into a formulation suitable for anticipated radiolabelling reactions. Results 61Cu saturation yields of 190 ± 33 MBq/μA from energetically thick natNi targets were measured. The average, decay-corrected, activity-based dissolution efficiency was 97.5 ± 1.4% with an average radiochemical yield of 90.4 ± 3.2% (N = 5). The isolated activity was collected approximately 65 min post end of bombardment in ~ 2 mL of 0.06 M HCl (HCl concentration was verified by titration). Quality control of the isolated [61Cu]CuCl2 (N = 5) measured 58Co content of (8.3 ± 0.6) × 10− 5% vs. 61Cu by activity, Ni separation factors ≥ (2.2 ± 1.8) × 106, EoB molar activities 85 ± 23 GBq/μmol and NOTA-based EoB apparent molar activities of 31 ± 8 MBq/nmol and 201 MBq/nmol for the 30 min and 3.3 h (N = 1) irradiations, respectively. Conclusion High purity 61Cu was produced with the developed automated method using a single-use, cassette-based approach. It was also applicable for 64Cu, as demonstrated with a single proof-of-concept 64Ni target production run. |
| first_indexed | 2024-12-11T03:59:01Z |
| format | Article |
| id | doaj.art-7b47395f590a4dcf889da6ea7014ce49 |
| institution | Directory Open Access Journal |
| issn | 2365-421X |
| language | English |
| last_indexed | 2024-12-11T03:59:01Z |
| publishDate | 2020-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | EJNMMI Radiopharmacy and Chemistry |
| spelling | doaj.art-7b47395f590a4dcf889da6ea7014ce492022-12-22T01:21:41ZengSpringerOpenEJNMMI Radiopharmacy and Chemistry2365-421X2020-11-015111410.1186/s41181-020-00108-7Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targetsJohan Svedjehed0Christopher J. Kutyreff1Jonathan W. Engle2Katherine Gagnon3Cyclotrons and TRACERcenter, GE Healthcare, GEMS PET Systems ABDepartment of Medical Physics, School of Medicine and Public Health, University of Wisconsin-MadisonDepartment of Medical Physics, School of Medicine and Public Health, University of Wisconsin-MadisonCyclotrons and TRACERcenter, GE Healthcare, GEMS PET Systems ABAbstract Background A need for improved, cassette-based automation of 61Cu separation from irradiated Ni targets was identified given the growing interest in theranostics, and generally lengthy separation chemistries for 64Cu/64Ni, upon which 61Cu chemistry is often based. Methods A method for separating 61Cu from irradiated natNi targets was therefore developed, with provision for target recycling. Following deuteron irradiation, electroplated natNi targets were remotely transferred from the cyclotron and dissolved in acid. The dissolved target solution was then transferred to an automated FASTlab chemistry module, where sequential TBP and TK201 (Triskem) resins isolated the [61Cu]CuCl2, removed Ni, Co, and Fe, and concentrated the product into a formulation suitable for anticipated radiolabelling reactions. Results 61Cu saturation yields of 190 ± 33 MBq/μA from energetically thick natNi targets were measured. The average, decay-corrected, activity-based dissolution efficiency was 97.5 ± 1.4% with an average radiochemical yield of 90.4 ± 3.2% (N = 5). The isolated activity was collected approximately 65 min post end of bombardment in ~ 2 mL of 0.06 M HCl (HCl concentration was verified by titration). Quality control of the isolated [61Cu]CuCl2 (N = 5) measured 58Co content of (8.3 ± 0.6) × 10− 5% vs. 61Cu by activity, Ni separation factors ≥ (2.2 ± 1.8) × 106, EoB molar activities 85 ± 23 GBq/μmol and NOTA-based EoB apparent molar activities of 31 ± 8 MBq/nmol and 201 MBq/nmol for the 30 min and 3.3 h (N = 1) irradiations, respectively. Conclusion High purity 61Cu was produced with the developed automated method using a single-use, cassette-based approach. It was also applicable for 64Cu, as demonstrated with a single proof-of-concept 64Ni target production run.http://link.springer.com/article/10.1186/s41181-020-00108-761Cu (radiocopper)Automation/automatedPETSolid targetDissolutionRecycling |
| spellingShingle | Johan Svedjehed Christopher J. Kutyreff Jonathan W. Engle Katherine Gagnon Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targets EJNMMI Radiopharmacy and Chemistry 61Cu (radiocopper) Automation/automated PET Solid target Dissolution Recycling |
| title | Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targets |
| title_full | Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targets |
| title_fullStr | Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targets |
| title_full_unstemmed | Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targets |
| title_short | Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targets |
| title_sort | automated cassette based isolation and formulation of high purity 61cu cucl2 from solid ni targets |
| topic | 61Cu (radiocopper) Automation/automated PET Solid target Dissolution Recycling |
| url | http://link.springer.com/article/10.1186/s41181-020-00108-7 |
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