Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma
Objective To investigate the expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and Netrin-1 in diffuse large B cell lymphoma (DLBCL). Methods The patients with freshly-diagnosed DLBCL in our hospital from May 2018 to May 2020 were enrolled in this study. According to...
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Editorial Office of Journal of Third Military Medical University
2021-04-01
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| Series: | Di-san junyi daxue xuebao |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202010172.htm |
| _version_ | 1818569286237552640 |
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| author | PU Xin ZHANG Yong CHEN Wen YANG Zailin YANG Sainan WEI Xia |
| author_facet | PU Xin ZHANG Yong CHEN Wen YANG Zailin YANG Sainan WEI Xia |
| author_sort | PU Xin |
| collection | DOAJ |
| description | Objective To investigate the expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and Netrin-1 in diffuse large B cell lymphoma (DLBCL). Methods The patients with freshly-diagnosed DLBCL in our hospital from May 2018 to May 2020 were enrolled in this study. According to the international prognostic index (IPI), they were divided into low-risk group (≤2 points) and high-risk group (>2 points), with 25 patients in each group. The expression of Ki-67, NGF, BDNF and Netrin-1 were detected by immunohistochemical assay. Flow cytometry was used to detect the proportions of regulatory T cells (Treg cells), regulatory B cells (Breg cells) and regulatory plasma cells (Preg cells). Results Compared with the low-risk group, the expression levels of Ki-67, NGF and Netrin-1 were significantly increased in the high-risk group (P < 0.05), but that of BDNF didn't differ. The proportion of Treg cells in peripheral blood was obviously increased (3.07±0.14 vs 3.72±0.18, P < 0.05), while those of Breg cells and Preg cells were not statistically changed (0.88±0.12 vs 1.06±0.10; 0.053±0.010 vs 0.054±0.009) in the high-risk group than the low-risk group. Correlation analysis results showed that NGF was positively correlated with Ki-67 proliferation index (r=0.720, P=0.008), but not with the proportions of Treg cells, Breg cells and Preg cells. Netrin-1 was positively correlated with Ki-67 proliferation index and proportion of Treg cells (r=0.804, P=0.002; r=0.608, P=0.036), but not with the proportions of Breg cells and Preg cells. There was no correlation of BDNF with Ki-67 proliferation index and above proportions. Conclusion The expression levels of NGF and Netrin-1 are significantly increased in DLBCL tissues and positively correlated with the proliferation of tumor cells and proportion of Treg cells. |
| first_indexed | 2024-12-14T06:46:08Z |
| format | Article |
| id | doaj.art-7b49177a411149f68cec9cdac5cb1153 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-14T06:46:08Z |
| publishDate | 2021-04-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-7b49177a411149f68cec9cdac5cb11532022-12-21T23:13:02ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042021-04-0143871572110.16016/j.1000-5404.202010172Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphomaPU Xin0ZHANG Yong1CHEN Wen2YANG Zailin3YANG Sainan4WEI Xia5Department of Hematology, the Third Affiliated Hospital of Chongqing Medical University (Gener Hospital), Chongqing, 401120, China Department of Hematology, the Third Affiliated Hospital of Chongqing Medical University (Gener Hospital), Chongqing, 401120, China Department of Hematology, the Third Affiliated Hospital of Chongqing Medical University (Gener Hospital), Chongqing, 401120, China Department of Hematology, the Third Affiliated Hospital of Chongqing Medical University (Gener Hospital), Chongqing, 401120, China Department of Hematology, the Third Affiliated Hospital of Chongqing Medical University (Gener Hospital), Chongqing, 401120, China Department of Hematology, the Third Affiliated Hospital of Chongqing Medical University (Gener Hospital), Chongqing, 401120, China Objective To investigate the expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and Netrin-1 in diffuse large B cell lymphoma (DLBCL). Methods The patients with freshly-diagnosed DLBCL in our hospital from May 2018 to May 2020 were enrolled in this study. According to the international prognostic index (IPI), they were divided into low-risk group (≤2 points) and high-risk group (>2 points), with 25 patients in each group. The expression of Ki-67, NGF, BDNF and Netrin-1 were detected by immunohistochemical assay. Flow cytometry was used to detect the proportions of regulatory T cells (Treg cells), regulatory B cells (Breg cells) and regulatory plasma cells (Preg cells). Results Compared with the low-risk group, the expression levels of Ki-67, NGF and Netrin-1 were significantly increased in the high-risk group (P < 0.05), but that of BDNF didn't differ. The proportion of Treg cells in peripheral blood was obviously increased (3.07±0.14 vs 3.72±0.18, P < 0.05), while those of Breg cells and Preg cells were not statistically changed (0.88±0.12 vs 1.06±0.10; 0.053±0.010 vs 0.054±0.009) in the high-risk group than the low-risk group. Correlation analysis results showed that NGF was positively correlated with Ki-67 proliferation index (r=0.720, P=0.008), but not with the proportions of Treg cells, Breg cells and Preg cells. Netrin-1 was positively correlated with Ki-67 proliferation index and proportion of Treg cells (r=0.804, P=0.002; r=0.608, P=0.036), but not with the proportions of Breg cells and Preg cells. There was no correlation of BDNF with Ki-67 proliferation index and above proportions. Conclusion The expression levels of NGF and Netrin-1 are significantly increased in DLBCL tissues and positively correlated with the proliferation of tumor cells and proportion of Treg cells.http://aammt.tmmu.edu.cn/Upload/rhtml/202010172.htmdiffuse large b cell lymphomanerve growth factorbrain-derived neurotrophic factornetrin-1 |
| spellingShingle | PU Xin ZHANG Yong CHEN Wen YANG Zailin YANG Sainan WEI Xia Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma Di-san junyi daxue xuebao diffuse large b cell lymphoma nerve growth factor brain-derived neurotrophic factor netrin-1 |
| title | Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma |
| title_full | Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma |
| title_fullStr | Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma |
| title_full_unstemmed | Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma |
| title_short | Expression of NGF, BDNF and Netrin-1 in diffuse large B cell lymphoma |
| title_sort | expression of ngf bdnf and netrin 1 in diffuse large b cell lymphoma |
| topic | diffuse large b cell lymphoma nerve growth factor brain-derived neurotrophic factor netrin-1 |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202010172.htm |
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