3′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion
3′-Daidzein sulfonate sodium (DSS) is a new synthetic water-soluble compound derived from daidzein, a soya isoflavone that plays regulatory roles in neurobiology. In this study, we hypothesized that the regulatory role of DSS in neurobiology exhibits therapeutic effects on hippocampal damage and mem...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=9;spage=1561;epage=1567;aulast=Li |
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| author | Xiao Li Rui-Zhen Liu Qi Zeng Zhi-Hua Huang Jian-Dong Zhang Zong-Liang Liu Jing Zeng Hai Xiao |
| author_facet | Xiao Li Rui-Zhen Liu Qi Zeng Zhi-Hua Huang Jian-Dong Zhang Zong-Liang Liu Jing Zeng Hai Xiao |
| author_sort | Xiao Li |
| collection | DOAJ |
| description | 3′-Daidzein sulfonate sodium (DSS) is a new synthetic water-soluble compound derived from daidzein, a soya isoflavone that plays regulatory roles in neurobiology. In this study, we hypothesized that the regulatory role of DSS in neurobiology exhibits therapeutic effects on hippocampal damage and memory impairment. To validate this hypothesis, we established rat models of chronic cerebral hypoperfusion (CCH) by the permanent occlusion of the common carotid arteries using the two-vessel occlusion method. Three weeks after modeling, rat models were intragastrically administered 0.1, 0.2, and 0.4 mg/kg DSS, once a day, for 5 successive weeks. The Morris water maze test was performed to investigate CCH-induced learning and memory deficits. TUNEL assay was used to analyze apoptosis in the hippocampal CA1, CA3 regions and dentate gyrus. Hematoxylin-eosin staining was performed to observe the morphology of neurons in the hippocampal CA1, CA3 regions and dentate gyrus. Western blot analysis was performed to investigate the phosphorylation of PKA, ERK1/2 and CREB in the hippocampal PKA/ERK1/2/CREB signaling pathway. Results showed that DSS treatment greatly improved the learning and memory deficits of rats with CCH, reduced apoptosis of neurons in the hippocampal CA1, CA3 regions and dentate gyrus, and increased the phosphorylation of PKA, ERK1/2, and CREB in the hippocampus. These findings suggest that DSS protects against CCH-induced memory impairment and hippocampal damage possibly through activating the PKA/ERK1/2/CREB signaling pathway. |
| first_indexed | 2024-12-12T16:49:05Z |
| format | Article |
| id | doaj.art-7b4931b6a5c9421bb8a0574b2fa9d753 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-12T16:49:05Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-7b4931b6a5c9421bb8a0574b2fa9d7532022-12-22T00:18:25ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742018-01-011391561156710.4103/1673-5374.2371193′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusionXiao LiRui-Zhen LiuQi ZengZhi-Hua HuangJian-Dong ZhangZong-Liang LiuJing ZengHai Xiao3′-Daidzein sulfonate sodium (DSS) is a new synthetic water-soluble compound derived from daidzein, a soya isoflavone that plays regulatory roles in neurobiology. In this study, we hypothesized that the regulatory role of DSS in neurobiology exhibits therapeutic effects on hippocampal damage and memory impairment. To validate this hypothesis, we established rat models of chronic cerebral hypoperfusion (CCH) by the permanent occlusion of the common carotid arteries using the two-vessel occlusion method. Three weeks after modeling, rat models were intragastrically administered 0.1, 0.2, and 0.4 mg/kg DSS, once a day, for 5 successive weeks. The Morris water maze test was performed to investigate CCH-induced learning and memory deficits. TUNEL assay was used to analyze apoptosis in the hippocampal CA1, CA3 regions and dentate gyrus. Hematoxylin-eosin staining was performed to observe the morphology of neurons in the hippocampal CA1, CA3 regions and dentate gyrus. Western blot analysis was performed to investigate the phosphorylation of PKA, ERK1/2 and CREB in the hippocampal PKA/ERK1/2/CREB signaling pathway. Results showed that DSS treatment greatly improved the learning and memory deficits of rats with CCH, reduced apoptosis of neurons in the hippocampal CA1, CA3 regions and dentate gyrus, and increased the phosphorylation of PKA, ERK1/2, and CREB in the hippocampus. These findings suggest that DSS protects against CCH-induced memory impairment and hippocampal damage possibly through activating the PKA/ERK1/2/CREB signaling pathway.http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=9;spage=1561;epage=1567;aulast=Linerve regenerationlearning and memory deficits; chronic cerebral hypoperfusion; 3?-daidzein sulfonate sodium; PKA/ERK1/2/ CREB signaling pathway; neuroprotection; hippocampus; neural regeneration |
| spellingShingle | Xiao Li Rui-Zhen Liu Qi Zeng Zhi-Hua Huang Jian-Dong Zhang Zong-Liang Liu Jing Zeng Hai Xiao 3′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion Neural Regeneration Research nerve regeneration learning and memory deficits; chronic cerebral hypoperfusion; 3?-daidzein sulfonate sodium; PKA/ERK1/2/ CREB signaling pathway; neuroprotection; hippocampus; neural regeneration |
| title | 3′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion |
| title_full | 3′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion |
| title_fullStr | 3′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion |
| title_full_unstemmed | 3′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion |
| title_short | 3′-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion |
| title_sort | 3 daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion |
| topic | nerve regeneration learning and memory deficits; chronic cerebral hypoperfusion; 3?-daidzein sulfonate sodium; PKA/ERK1/2/ CREB signaling pathway; neuroprotection; hippocampus; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=9;spage=1561;epage=1567;aulast=Li |
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