The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines
Newcastle disease (ND) is a highly contagious viral respiratory and neurological disease that has a severe impact on poultry production worldwide. In the present study, an expression platform was established for the transient production in N.bethamiana of ND virus-like particles (VLPs) for use as va...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1130910/full |
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| author | Tanja Smith Tanja Smith Martha M. O’Kennedy Martha M. O’Kennedy Craig S. Ross Nicola S. Lewis Nicola S. Lewis Celia Abolnik |
| author_facet | Tanja Smith Tanja Smith Martha M. O’Kennedy Martha M. O’Kennedy Craig S. Ross Nicola S. Lewis Nicola S. Lewis Celia Abolnik |
| author_sort | Tanja Smith |
| collection | DOAJ |
| description | Newcastle disease (ND) is a highly contagious viral respiratory and neurological disease that has a severe impact on poultry production worldwide. In the present study, an expression platform was established for the transient production in N.bethamiana of ND virus-like particles (VLPs) for use as vaccines against ND. The expression of the ND Fusion (F) and/or Hemagglutinin-neuraminidase (HN) proteins of a genotype VII.2 strain formed ND VLPs in planta as visualized under the transmission electron microscope, and HN-containing VLPs agglutinated chicken erythrocytes with hemagglutination (HA) titres of up to 13 log2.The immunogenicity of the partially-purified ND VLPs was confirmed in specific-pathogen-free White leghorn chickens. Birds receiving a single intramuscular immunization with 1024 HA units (10 log2) of the F/HN ND VLPs administered with 20% [v/v] Emulsigen®-P adjuvant, seroconverted after 14 days with F- and HN-specific antibodies at ELISA titres of 5705.17 and HI geometric mean titres (GMTs) of 6.2 log2, respectively. Furthermore, these ND-specific antibodies successfully inhibited viral replication in vitro of two antigenically closely-related ND virus isolates, with virus-neutralization test GMTs of 3.47 and 3.4, respectively. Plant-produced ND VLPs have great potential as antigen-matched vaccines for poultry and other avian species that are highly immunogenic, cost-effective, and facilitate prompt updating to ensure improved protection against emerging ND field viruses. |
| first_indexed | 2024-04-10T09:55:26Z |
| format | Article |
| id | doaj.art-7b496f62aba740ba824829f63dfcb56c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T09:55:26Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7b496f62aba740ba824829f63dfcb56c2023-02-16T12:26:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.11309101130910The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccinesTanja Smith0Tanja Smith1Martha M. O’Kennedy2Martha M. O’Kennedy3Craig S. Ross4Nicola S. Lewis5Nicola S. Lewis6Celia Abolnik7Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Gauteng, Pretoria, South AfricaNext Generation Health, Council for Scientific and Industrial Research, Pretoria, South AfricaDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Gauteng, Pretoria, South AfricaNext Generation Health, Council for Scientific and Industrial Research, Pretoria, South AfricaAvian Virology Department, Animal and Plant Health Agency (APHA), Woodham Lane, Addlestone, United KingdomAvian Virology Department, Animal and Plant Health Agency (APHA), Woodham Lane, Addlestone, United KingdomDepartment of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, United KingdomDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Gauteng, Pretoria, South AfricaNewcastle disease (ND) is a highly contagious viral respiratory and neurological disease that has a severe impact on poultry production worldwide. In the present study, an expression platform was established for the transient production in N.bethamiana of ND virus-like particles (VLPs) for use as vaccines against ND. The expression of the ND Fusion (F) and/or Hemagglutinin-neuraminidase (HN) proteins of a genotype VII.2 strain formed ND VLPs in planta as visualized under the transmission electron microscope, and HN-containing VLPs agglutinated chicken erythrocytes with hemagglutination (HA) titres of up to 13 log2.The immunogenicity of the partially-purified ND VLPs was confirmed in specific-pathogen-free White leghorn chickens. Birds receiving a single intramuscular immunization with 1024 HA units (10 log2) of the F/HN ND VLPs administered with 20% [v/v] Emulsigen®-P adjuvant, seroconverted after 14 days with F- and HN-specific antibodies at ELISA titres of 5705.17 and HI geometric mean titres (GMTs) of 6.2 log2, respectively. Furthermore, these ND-specific antibodies successfully inhibited viral replication in vitro of two antigenically closely-related ND virus isolates, with virus-neutralization test GMTs of 3.47 and 3.4, respectively. Plant-produced ND VLPs have great potential as antigen-matched vaccines for poultry and other avian species that are highly immunogenic, cost-effective, and facilitate prompt updating to ensure improved protection against emerging ND field viruses.https://www.frontiersin.org/articles/10.3389/fpls.2023.1130910/fullNewcastle disease virus (NDV)virus-like particles (VLPs)Nicotiana benthamianaagroinfiltrationpoultry vaccineimmunogenicity |
| spellingShingle | Tanja Smith Tanja Smith Martha M. O’Kennedy Martha M. O’Kennedy Craig S. Ross Nicola S. Lewis Nicola S. Lewis Celia Abolnik The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines Frontiers in Plant Science Newcastle disease virus (NDV) virus-like particles (VLPs) Nicotiana benthamiana agroinfiltration poultry vaccine immunogenicity |
| title | The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines |
| title_full | The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines |
| title_fullStr | The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines |
| title_full_unstemmed | The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines |
| title_short | The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines |
| title_sort | production of newcastle disease virus like particles in nicotiana benthamiana as potential vaccines |
| topic | Newcastle disease virus (NDV) virus-like particles (VLPs) Nicotiana benthamiana agroinfiltration poultry vaccine immunogenicity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1130910/full |
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