Kidney stone formers have more renal parenchymal crystals than non-stone formers, particularly in the papilla region
Abstract Background We investigated the renoprotective ability of healthy people against kidney stone formation. To clarify intratubular crystal kinetics and processing in human kidneys, we performed a quantitative and morphological observation of nephrectomized renal parenchyma tissues. Methods Cli...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-03-01
|
| Series: | BMC Urology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12894-018-0331-x |
| _version_ | 1811325588410990592 |
|---|---|
| author | Atsushi Okada Shuzo Hamamoto Kazumi Taguchi Rei Unno Teruaki Sugino Ryosuke Ando Kentaro Mizuno Keiichi Tozawa Kenjiro Kohri Takahiro Yasui |
| author_facet | Atsushi Okada Shuzo Hamamoto Kazumi Taguchi Rei Unno Teruaki Sugino Ryosuke Ando Kentaro Mizuno Keiichi Tozawa Kenjiro Kohri Takahiro Yasui |
| author_sort | Atsushi Okada |
| collection | DOAJ |
| description | Abstract Background We investigated the renoprotective ability of healthy people against kidney stone formation. To clarify intratubular crystal kinetics and processing in human kidneys, we performed a quantitative and morphological observation of nephrectomized renal parenchyma tissues. Methods Clinical data and pathological samples from 60 patients who underwent radical nephrectomy for renal cancer were collected from June 2004 to June 2010. The patients were retrospectively classified as stone formers (SFs; n = 30, kidney stones detected by preoperative computed tomography) and non-stone formers (NSFs; n = 30, no kidney stone history). The morphology of parenchymal intratubular crystals and kidney stone-related gene and protein expression levels were examined in noncancerous renal sections from both groups. Results SFs had a higher smoking rate (P = 0.0097); lower red blood cell, hemoglobin, and hematocrit values; and higher urinary red blood cell, white blood cell, and bacterial counts than NSFs. Scanning electron microscopy revealed calcium-containing crystal deposits and crystal attachment to the renal tubular lumen in both groups. Both groups demonstrated crystal transmigration from the tubular lumen to the interstitium. The crystal diffusion analysis indicated a significantly higher crystal existing ratio in the medulla and papilla of SFs and a significantly higher number of papillary crystal deposits in SFs than NSFs. The expression analysis indicated relatively high osteopontin and CD68, low superoxide dismutase, and significantly lower Tamm–Horsfall protein expression levels in SFs. Multivariate logistic regression analysis involving the above factors found the presence of renal papillary crystals as a significant independent factor related to SFs (odds ratio 5.55, 95% confidence interval 1.08–37.18, P = 0.0395). Conclusions Regardless of stone formation, intratubular crystals in the renal parenchyma seem to transmigrate to the interstitium. SFs may have reduced ability to eliminate renal parenchymal crystals, particularly those in the papilla region, than NSFs with associated gene expression profiles. |
| first_indexed | 2024-04-13T14:36:08Z |
| format | Article |
| id | doaj.art-7b4a22f9c70e4bb581fe9f021f459c68 |
| institution | Directory Open Access Journal |
| issn | 1471-2490 |
| language | English |
| last_indexed | 2024-04-13T14:36:08Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Urology |
| spelling | doaj.art-7b4a22f9c70e4bb581fe9f021f459c682022-12-22T02:43:02ZengBMCBMC Urology1471-24902018-03-0118111010.1186/s12894-018-0331-xKidney stone formers have more renal parenchymal crystals than non-stone formers, particularly in the papilla regionAtsushi Okada0Shuzo Hamamoto1Kazumi Taguchi2Rei Unno3Teruaki Sugino4Ryosuke Ando5Kentaro Mizuno6Keiichi Tozawa7Kenjiro Kohri8Takahiro Yasui9Department of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesDepartment of Nephro-urology, Nagoya City University Graduate School of Medical SciencesAbstract Background We investigated the renoprotective ability of healthy people against kidney stone formation. To clarify intratubular crystal kinetics and processing in human kidneys, we performed a quantitative and morphological observation of nephrectomized renal parenchyma tissues. Methods Clinical data and pathological samples from 60 patients who underwent radical nephrectomy for renal cancer were collected from June 2004 to June 2010. The patients were retrospectively classified as stone formers (SFs; n = 30, kidney stones detected by preoperative computed tomography) and non-stone formers (NSFs; n = 30, no kidney stone history). The morphology of parenchymal intratubular crystals and kidney stone-related gene and protein expression levels were examined in noncancerous renal sections from both groups. Results SFs had a higher smoking rate (P = 0.0097); lower red blood cell, hemoglobin, and hematocrit values; and higher urinary red blood cell, white blood cell, and bacterial counts than NSFs. Scanning electron microscopy revealed calcium-containing crystal deposits and crystal attachment to the renal tubular lumen in both groups. Both groups demonstrated crystal transmigration from the tubular lumen to the interstitium. The crystal diffusion analysis indicated a significantly higher crystal existing ratio in the medulla and papilla of SFs and a significantly higher number of papillary crystal deposits in SFs than NSFs. The expression analysis indicated relatively high osteopontin and CD68, low superoxide dismutase, and significantly lower Tamm–Horsfall protein expression levels in SFs. Multivariate logistic regression analysis involving the above factors found the presence of renal papillary crystals as a significant independent factor related to SFs (odds ratio 5.55, 95% confidence interval 1.08–37.18, P = 0.0395). Conclusions Regardless of stone formation, intratubular crystals in the renal parenchyma seem to transmigrate to the interstitium. SFs may have reduced ability to eliminate renal parenchymal crystals, particularly those in the papilla region, than NSFs with associated gene expression profiles.http://link.springer.com/article/10.1186/s12894-018-0331-xKidney stonesMacrophagesOsteopontinOxidative stressTamm-Horsfall protein |
| spellingShingle | Atsushi Okada Shuzo Hamamoto Kazumi Taguchi Rei Unno Teruaki Sugino Ryosuke Ando Kentaro Mizuno Keiichi Tozawa Kenjiro Kohri Takahiro Yasui Kidney stone formers have more renal parenchymal crystals than non-stone formers, particularly in the papilla region BMC Urology Kidney stones Macrophages Osteopontin Oxidative stress Tamm-Horsfall protein |
| title | Kidney stone formers have more renal parenchymal crystals than non-stone formers, particularly in the papilla region |
| title_full | Kidney stone formers have more renal parenchymal crystals than non-stone formers, particularly in the papilla region |
| title_fullStr | Kidney stone formers have more renal parenchymal crystals than non-stone formers, particularly in the papilla region |
| title_full_unstemmed | Kidney stone formers have more renal parenchymal crystals than non-stone formers, particularly in the papilla region |
| title_short | Kidney stone formers have more renal parenchymal crystals than non-stone formers, particularly in the papilla region |
| title_sort | kidney stone formers have more renal parenchymal crystals than non stone formers particularly in the papilla region |
| topic | Kidney stones Macrophages Osteopontin Oxidative stress Tamm-Horsfall protein |
| url | http://link.springer.com/article/10.1186/s12894-018-0331-x |
| work_keys_str_mv | AT atsushiokada kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT shuzohamamoto kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT kazumitaguchi kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT reiunno kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT teruakisugino kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT ryosukeando kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT kentaromizuno kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT keiichitozawa kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT kenjirokohri kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion AT takahiroyasui kidneystoneformershavemorerenalparenchymalcrystalsthannonstoneformersparticularlyinthepapillaregion |