Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope
Lensless holographic microscope (LHM) is an emerging very promising technology that provides high-quality imaging and analysis of biological samples without utilizing any lens for imaging. Due to its small size and reduced price, LHM can be a very useful tool for the point-of-care diagnosis of disea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/2/553 |
| _version_ | 1797490472164261888 |
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| author | Jose Angel Picazo-Bueno Karina Trindade Martin Sanz Vicente Micó |
| author_facet | Jose Angel Picazo-Bueno Karina Trindade Martin Sanz Vicente Micó |
| author_sort | Jose Angel Picazo-Bueno |
| collection | DOAJ |
| description | Lensless holographic microscope (LHM) is an emerging very promising technology that provides high-quality imaging and analysis of biological samples without utilizing any lens for imaging. Due to its small size and reduced price, LHM can be a very useful tool for the point-of-care diagnosis of diseases, sperm assessment, or microfluidics, among others, not only employed in advanced laboratories but also in poor and/or remote areas. Recently, several LHMs have been reported in the literature. However, complete characterization of their optical parameters remains not much presented yet. Hence, we present a complete analysis of the performance of a compact, reduced cost, and high-resolution LHM. In particular, optical parameters such as lateral and axial resolutions, lateral magnification, and field of view are discussed into detail, comparing the experimental results with the expected theoretical values for different layout configurations. We use high-resolution amplitude and phase test targets and several microbeads to characterize the proposed microscope. This characterization is used to define a balanced and matched setup showing a good compromise between the involved parameters. Finally, such a microscope is utilized for visualization of static, as well as dynamic biosamples. |
| first_indexed | 2024-03-10T00:33:28Z |
| format | Article |
| id | doaj.art-7b4e076ca32d4bb3a61b57d588379606 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T00:33:28Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-7b4e076ca32d4bb3a61b57d5883796062023-11-23T15:20:34ZengMDPI AGSensors1424-82202022-01-0122255310.3390/s22020553Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic MicroscopeJose Angel Picazo-Bueno0Karina Trindade1Martin Sanz2Vicente Micó3Optics and Optometry and Vision Science, University of Valencia, 46100 Burjassot, SpainOptics and Optometry and Vision Science, University of Valencia, 46100 Burjassot, SpainOptics and Optometry and Vision Science, University of Valencia, 46100 Burjassot, SpainOptics and Optometry and Vision Science, University of Valencia, 46100 Burjassot, SpainLensless holographic microscope (LHM) is an emerging very promising technology that provides high-quality imaging and analysis of biological samples without utilizing any lens for imaging. Due to its small size and reduced price, LHM can be a very useful tool for the point-of-care diagnosis of diseases, sperm assessment, or microfluidics, among others, not only employed in advanced laboratories but also in poor and/or remote areas. Recently, several LHMs have been reported in the literature. However, complete characterization of their optical parameters remains not much presented yet. Hence, we present a complete analysis of the performance of a compact, reduced cost, and high-resolution LHM. In particular, optical parameters such as lateral and axial resolutions, lateral magnification, and field of view are discussed into detail, comparing the experimental results with the expected theoretical values for different layout configurations. We use high-resolution amplitude and phase test targets and several microbeads to characterize the proposed microscope. This characterization is used to define a balanced and matched setup showing a good compromise between the involved parameters. Finally, such a microscope is utilized for visualization of static, as well as dynamic biosamples.https://www.mdpi.com/1424-8220/22/2/553in-line holographic microscopydigital holographyGabor holographylensless microscopedigital image processingmedical and biological imaging |
| spellingShingle | Jose Angel Picazo-Bueno Karina Trindade Martin Sanz Vicente Micó Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope Sensors in-line holographic microscopy digital holography Gabor holography lensless microscope digital image processing medical and biological imaging |
| title | Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope |
| title_full | Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope |
| title_fullStr | Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope |
| title_full_unstemmed | Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope |
| title_short | Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope |
| title_sort | design calibration and application of a robust cost effective and high resolution lensless holographic microscope |
| topic | in-line holographic microscopy digital holography Gabor holography lensless microscope digital image processing medical and biological imaging |
| url | https://www.mdpi.com/1424-8220/22/2/553 |
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