The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol
Objective: To investigate the effect of asiatic acid (AA) on the differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol. Methods: Human neuroblastoma SH-SY5Y cells were either exposed to different concentrations of AA or treated with different doses of cholesterol to revea...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-05-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169116305056 |
| _version_ | 1828411893713207296 |
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| author | Kanchanat Ternchoocheep Damrassamon Surangkul Sukhgij Ysothonsreekul |
| author_facet | Kanchanat Ternchoocheep Damrassamon Surangkul Sukhgij Ysothonsreekul |
| author_sort | Kanchanat Ternchoocheep |
| collection | DOAJ |
| description | Objective: To investigate the effect of asiatic acid (AA) on the differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol.
Methods: Human neuroblastoma SH-SY5Y cells were either exposed to different concentrations of AA or treated with different doses of cholesterol to reveal their responding viability by MTT assay. The selective 1 μmol/L concentration of AA was then used to test for either the protective or the recovery effects on the cells treated with 250 μmol/L concentration of cholesterol.
Results: AA has a propensity to directly increase the viability of differentiated human neuroblastoma SH-SY5Y cells. Cholesterol has significant cytotoxic effect on those cells in a concentration-dependent manner. AA has the ability to slightly recover the viability of the differentiated culture cytotoxic-induced by cholesterol but could not protect those cells from cytotoxic-induced by cholesterol.
Conclusions: High concentrations of cholesterol were observed to be harmful to the neurons and AA had a slight effect of reducing neuronal death caused by cholesterol. |
| first_indexed | 2024-12-10T12:38:54Z |
| format | Article |
| id | doaj.art-7b4e6c49b35646ce8a418ca4d48f7488 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-10T12:38:54Z |
| publishDate | 2017-05-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-7b4e6c49b35646ce8a418ca4d48f74882022-12-22T01:48:35ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-05-017541642010.1016/j.apjtb.2017.01.012The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterolKanchanat Ternchoocheep0Damrassamon Surangkul1Sukhgij Ysothonsreekul2Department of Biochemistry, Faculty of Medical Science, Naresaun University, Muang, Phitsanulok 65000, ThailandDepartment of Biochemistry, Faculty of Medical Science, Naresaun University, Muang, Phitsanulok 65000, ThailandDepartment of Scientific Research, PTT Global Chemical Public Company Limited, Noenphra, Mueang Rayong, Rayong 21150, ThailandObjective: To investigate the effect of asiatic acid (AA) on the differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol. Methods: Human neuroblastoma SH-SY5Y cells were either exposed to different concentrations of AA or treated with different doses of cholesterol to reveal their responding viability by MTT assay. The selective 1 μmol/L concentration of AA was then used to test for either the protective or the recovery effects on the cells treated with 250 μmol/L concentration of cholesterol. Results: AA has a propensity to directly increase the viability of differentiated human neuroblastoma SH-SY5Y cells. Cholesterol has significant cytotoxic effect on those cells in a concentration-dependent manner. AA has the ability to slightly recover the viability of the differentiated culture cytotoxic-induced by cholesterol but could not protect those cells from cytotoxic-induced by cholesterol. Conclusions: High concentrations of cholesterol were observed to be harmful to the neurons and AA had a slight effect of reducing neuronal death caused by cholesterol.http://www.sciencedirect.com/science/article/pii/S2221169116305056Asiatic acidCholesterolSH-SY5Y cellsCell viability |
| spellingShingle | Kanchanat Ternchoocheep Damrassamon Surangkul Sukhgij Ysothonsreekul The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol Asian Pacific Journal of Tropical Biomedicine Asiatic acid Cholesterol SH-SY5Y cells Cell viability |
| title | The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol |
| title_full | The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol |
| title_fullStr | The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol |
| title_full_unstemmed | The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol |
| title_short | The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol |
| title_sort | recovery and protective effects of asiatic acid on differentiated human neuroblastoma sh sy5y cells cytotoxic induced by cholesterol |
| topic | Asiatic acid Cholesterol SH-SY5Y cells Cell viability |
| url | http://www.sciencedirect.com/science/article/pii/S2221169116305056 |
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