Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation
Uterine regeneration using decellularization scaffolds provides a novel treatment for uterine factor infertility. Decellularized scaffolds require maximal removal of cellular components and minimal damage to the extracellular matrix (ECM). Among many decellularization methods, the hydrostatic pressu...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/7/814 |
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| author | Dongzhe Wang Narintadeach Charoensombut Kinyoshi Kawabata Tsuyoshi Kimura Akio Kishida Takashi Ushida Katsuko S. Furukawa |
| author_facet | Dongzhe Wang Narintadeach Charoensombut Kinyoshi Kawabata Tsuyoshi Kimura Akio Kishida Takashi Ushida Katsuko S. Furukawa |
| author_sort | Dongzhe Wang |
| collection | DOAJ |
| description | Uterine regeneration using decellularization scaffolds provides a novel treatment for uterine factor infertility. Decellularized scaffolds require maximal removal of cellular components and minimal damage to the extracellular matrix (ECM). Among many decellularization methods, the hydrostatic pressure (HP) method stands out due to its low cytotoxicity and superior ECM preservation compared to the traditional detergent methods. Conventionally, 980 MPa was utilized in HP decellularization, including the first successful implementation of uterine decellularization previously reported by our team. However, structural protein denaturation caused by exceeding pressure led to a limited regeneration outcome in our previous research. This factor urged the study on the effects of pressure conditions in HP methods on decellularized scaffolds. The authors, therefore, fabricated a decellularized uterine scaffold at varying pressure conditions and evaluated the scaffold qualities from the perspective of cell removal and ECM preservation. The results show that by using lower decellularization pressure conditions of 250 MPa, uterine tissue can be decellularized with more preserved structural protein and mechanical properties, which is considered to be promising for decellularized uterine scaffold fabrication applications. |
| first_indexed | 2024-03-11T01:17:40Z |
| format | Article |
| id | doaj.art-7b513af9411a46d784e690297f9c8608 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T01:17:40Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-7b513af9411a46d784e690297f9c86082023-11-18T18:21:43ZengMDPI AGBioengineering2306-53542023-07-0110781410.3390/bioengineering10070814Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein PreservationDongzhe Wang0Narintadeach Charoensombut1Kinyoshi Kawabata2Tsuyoshi Kimura3Akio Kishida4Takashi Ushida5Katsuko S. Furukawa6Department of Mechanical Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, JapanDepartment of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, JapanDepartment of Mechanical Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, JapanDepartment of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, JapanDepartment of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, JapanDepartment of Mechanical Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, JapanDepartment of Mechanical Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, JapanUterine regeneration using decellularization scaffolds provides a novel treatment for uterine factor infertility. Decellularized scaffolds require maximal removal of cellular components and minimal damage to the extracellular matrix (ECM). Among many decellularization methods, the hydrostatic pressure (HP) method stands out due to its low cytotoxicity and superior ECM preservation compared to the traditional detergent methods. Conventionally, 980 MPa was utilized in HP decellularization, including the first successful implementation of uterine decellularization previously reported by our team. However, structural protein denaturation caused by exceeding pressure led to a limited regeneration outcome in our previous research. This factor urged the study on the effects of pressure conditions in HP methods on decellularized scaffolds. The authors, therefore, fabricated a decellularized uterine scaffold at varying pressure conditions and evaluated the scaffold qualities from the perspective of cell removal and ECM preservation. The results show that by using lower decellularization pressure conditions of 250 MPa, uterine tissue can be decellularized with more preserved structural protein and mechanical properties, which is considered to be promising for decellularized uterine scaffold fabrication applications.https://www.mdpi.com/2306-5354/10/7/814uterine regenerationtissue engineeringdecellularized scaffoldhydrostatic pressure |
| spellingShingle | Dongzhe Wang Narintadeach Charoensombut Kinyoshi Kawabata Tsuyoshi Kimura Akio Kishida Takashi Ushida Katsuko S. Furukawa Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation Bioengineering uterine regeneration tissue engineering decellularized scaffold hydrostatic pressure |
| title | Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation |
| title_full | Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation |
| title_fullStr | Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation |
| title_full_unstemmed | Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation |
| title_short | Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation |
| title_sort | effect of pressure conditions in uterine decellularization using hydrostatic pressure on structural protein preservation |
| topic | uterine regeneration tissue engineering decellularized scaffold hydrostatic pressure |
| url | https://www.mdpi.com/2306-5354/10/7/814 |
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