Phytochemical, Morphological and Genetic Characterisation of <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire and <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link
The present study is based on a multidisciplinary approach carried out for the first time on <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> and <i>Anacyclus pyrethrum</i> var. <i>depressus</i>, two varieties from the endemic and endangered medicinal...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/14/5378 |
| _version_ | 1797588016269623296 |
|---|---|
| author | Fatima Zahra Jawhari Hamada Imtara Nabil Radouane Abdelfattah El Moussaoui Imane Es-safi Amal Amaghnouje Mashail N. AlZain Omer Noman Mohammad Khalid Parvez Dalila Bousta Amina Bari |
| author_facet | Fatima Zahra Jawhari Hamada Imtara Nabil Radouane Abdelfattah El Moussaoui Imane Es-safi Amal Amaghnouje Mashail N. AlZain Omer Noman Mohammad Khalid Parvez Dalila Bousta Amina Bari |
| author_sort | Fatima Zahra Jawhari |
| collection | DOAJ |
| description | The present study is based on a multidisciplinary approach carried out for the first time on <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> and <i>Anacyclus pyrethrum</i> var. <i>depressus</i>, two varieties from the endemic and endangered medicinal species listed in the IUCN red list, <i>Anacyclus pyrethrum</i> (L.) Link. Therefore, morphological, phytochemical, and genetic characterisations were carried out in the present work. Morphological characterisation was established based on 23 qualitative and quantitative characters describing the vegetative and floral parts. The phytochemical compounds were determined by UHPLC. Genetic characterisation of extracted DNA was subjected to PCR using two sets of universal primers, rbcL a-f/rbcL a-R and rpocL1-2/rpocL1-4, followed by sequencing analysis using the Sanger method. The results revealed a significant difference between the two varieties studied. Furthermore, phytochemical analysis of the studied extracts revealed a quantitative and qualitative variation in the chemical profile, as well as the presence of interesting compounds, including new compounds that have never been reported in <i>A. pyrethrum</i>. The phylogenetic analysis of the DNA sequences indicated a similarity percentage of 91%. Based on the morphological characterisation and congruence with the phytochemical characterisation and molecular data, we can confirm that <i>A. pyrethrum</i> var. <i>pyrethrum</i> and <i>A. pyrethrum</i> var. <i>depressus</i> represent two different taxa. |
| first_indexed | 2024-03-11T00:47:00Z |
| format | Article |
| id | doaj.art-7b52607539a4436993fc3258ec2649e1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:47:00Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7b52607539a4436993fc3258ec2649e12023-11-18T20:41:15ZengMDPI AGMolecules1420-30492023-07-012814537810.3390/molecules28145378Phytochemical, Morphological and Genetic Characterisation of <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire and <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) LinkFatima Zahra Jawhari0Hamada Imtara1Nabil Radouane2Abdelfattah El Moussaoui3Imane Es-safi4Amal Amaghnouje5Mashail N. AlZain6Omer Noman7Mohammad Khalid Parvez8Dalila Bousta9Amina Bari10Laboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, MoroccoFaculty of Sciences, Arab American University Palestine, Jenin P.O. Box 240, PalestineAfrican Genome Center, Mohammed VI Polytechnic University (UM6P), Lot 660, Hay Moulay Rachid, Ben Guerir 43150, MoroccoLaboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, MoroccoLaboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, MoroccoLaboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, MoroccoDepartment of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11761, Saudi ArabiaDepartment of Pharmaceutical Biology, Institute of Pharmacy, University of Greifswald, 17489 Greifswald, GermanyDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaLaboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, MoroccoLaboratory of Biotechnology, Environment, Agri-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah (USMBA) University, P.O. Box 2202, Fez 30000, MoroccoThe present study is based on a multidisciplinary approach carried out for the first time on <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> and <i>Anacyclus pyrethrum</i> var. <i>depressus</i>, two varieties from the endemic and endangered medicinal species listed in the IUCN red list, <i>Anacyclus pyrethrum</i> (L.) Link. Therefore, morphological, phytochemical, and genetic characterisations were carried out in the present work. Morphological characterisation was established based on 23 qualitative and quantitative characters describing the vegetative and floral parts. The phytochemical compounds were determined by UHPLC. Genetic characterisation of extracted DNA was subjected to PCR using two sets of universal primers, rbcL a-f/rbcL a-R and rpocL1-2/rpocL1-4, followed by sequencing analysis using the Sanger method. The results revealed a significant difference between the two varieties studied. Furthermore, phytochemical analysis of the studied extracts revealed a quantitative and qualitative variation in the chemical profile, as well as the presence of interesting compounds, including new compounds that have never been reported in <i>A. pyrethrum</i>. The phylogenetic analysis of the DNA sequences indicated a similarity percentage of 91%. Based on the morphological characterisation and congruence with the phytochemical characterisation and molecular data, we can confirm that <i>A. pyrethrum</i> var. <i>pyrethrum</i> and <i>A. pyrethrum</i> var. <i>depressus</i> represent two different taxa.https://www.mdpi.com/1420-3049/28/14/5378<i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link<i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Mairemorphological characterisationphysicochemical characterisationgenetic characterisation |
| spellingShingle | Fatima Zahra Jawhari Hamada Imtara Nabil Radouane Abdelfattah El Moussaoui Imane Es-safi Amal Amaghnouje Mashail N. AlZain Omer Noman Mohammad Khalid Parvez Dalila Bousta Amina Bari Phytochemical, Morphological and Genetic Characterisation of <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire and <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link Molecules <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire morphological characterisation physicochemical characterisation genetic characterisation |
| title | Phytochemical, Morphological and Genetic Characterisation of <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire and <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link |
| title_full | Phytochemical, Morphological and Genetic Characterisation of <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire and <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link |
| title_fullStr | Phytochemical, Morphological and Genetic Characterisation of <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire and <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link |
| title_full_unstemmed | Phytochemical, Morphological and Genetic Characterisation of <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire and <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link |
| title_short | Phytochemical, Morphological and Genetic Characterisation of <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire and <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link |
| title_sort | phytochemical morphological and genetic characterisation of i anacyclus pyrethrum i var i depressus i ball maire and i anacyclus pyrethrum i var i pyrethrum i l link |
| topic | <i>Anacyclus pyrethrum</i> var. <i>pyrethrum</i> (L.) Link <i>Anacyclus pyrethrum</i> var. <i>depressus</i> (Ball.) Maire morphological characterisation physicochemical characterisation genetic characterisation |
| url | https://www.mdpi.com/1420-3049/28/14/5378 |
| work_keys_str_mv | AT fatimazahrajawhari phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT hamadaimtara phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT nabilradouane phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT abdelfattahelmoussaoui phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT imaneessafi phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT amalamaghnouje phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT mashailnalzain phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT omernoman phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT mohammadkhalidparvez phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT dalilabousta phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink AT aminabari phytochemicalmorphologicalandgeneticcharacterisationofianacycluspyrethrumivaridepressusiballmaireandianacycluspyrethrumivaripyrethrumillink |