Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca2+ influx and differentiation
Treating neurodegenerative diseases, e.g., Alzheimer's Disease, remains a significant challenge due to the limited neuroregeneration rate in the brain. The objective of this study is to evaluate the hypothesis that external magnetic field (MF) stimulation of nerve growth factor functionalized s...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-08-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23000075 |
| _version_ | 1797203920545644544 |
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| author | Elias Georgas Muzhaozi Yuan Jingfan Chen Ya Wang Yi-Xian Qin |
| author_facet | Elias Georgas Muzhaozi Yuan Jingfan Chen Ya Wang Yi-Xian Qin |
| author_sort | Elias Georgas |
| collection | DOAJ |
| description | Treating neurodegenerative diseases, e.g., Alzheimer's Disease, remains a significant challenge due to the limited neuroregeneration rate in the brain. The objective of this study is to evaluate the hypothesis that external magnetic field (MF) stimulation of nerve growth factor functionalized superparamagnetic iron oxide-gold (NGF-SPIO-Au) nanoparticles (NPs) can induce Ca2+ influx, membrane depolarization, and enhance neuron differentiation with dynamic MF (DMF) outperforming static MF (SMF) regulation. We showed the that total intracellular Ca2+ influx of PC-12 cells was improved by 300% and 535% by the stimulation of DMF (1 Hz, 0.5 T, 30min) with NGF-SPIO-Au NPs compared to DMF alone and SMF with NGF-SPIO-Au NPs, respectively, which was attributed to successive membrane depolarization. Cellular uptake performed with the application of sodium azide proved that DMF enhanced cellular uptake of NGF-SPIO-Au NPs via endocytosis. In addition, DMF upregulated both the neural differentiation marker (β3-tubulin) and the cell adhesive molecule (integrin-β1) with the existence of NGF-SPIO-Au NPs, while SMF did not show these effects. The results imply that noninvasive DMF-stimulated NPs can regulate intracellular Ca2+ influx and enhance neuron differentiation and neuroregeneration rate. |
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| format | Article |
| id | doaj.art-7b52d1eec3fb46a9877f6e594675c448 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:27:00Z |
| publishDate | 2023-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-7b52d1eec3fb46a9877f6e594675c4482024-04-16T21:57:06ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-08-0126478489Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca2+ influx and differentiationElias Georgas0Muzhaozi Yuan1Jingfan Chen2Ya Wang3Yi-Xian Qin4Department of Biomedical Engineering, The State University of New York at Stony Brook, Stony Brook, NY, United StatesJ. Mike Walker ’66 Department of Mechanical Engineering, Texas A&M University, College Station, TX, United StatesJ. Mike Walker ’66 Department of Mechanical Engineering, Texas A&M University, College Station, TX, United StatesJ. Mike Walker ’66 Department of Mechanical Engineering, Texas A&M University, College Station, TX, United States; Department of Biomedical Engineering, Texas A&M University, College Station, TX, United States; Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, United StatesDepartment of Biomedical Engineering, The State University of New York at Stony Brook, Stony Brook, NY, United States; Corresponding author. Department of Biomedical Engineering, The State University of New York at Stony Brook, Stony Brook, NY, United States.Treating neurodegenerative diseases, e.g., Alzheimer's Disease, remains a significant challenge due to the limited neuroregeneration rate in the brain. The objective of this study is to evaluate the hypothesis that external magnetic field (MF) stimulation of nerve growth factor functionalized superparamagnetic iron oxide-gold (NGF-SPIO-Au) nanoparticles (NPs) can induce Ca2+ influx, membrane depolarization, and enhance neuron differentiation with dynamic MF (DMF) outperforming static MF (SMF) regulation. We showed the that total intracellular Ca2+ influx of PC-12 cells was improved by 300% and 535% by the stimulation of DMF (1 Hz, 0.5 T, 30min) with NGF-SPIO-Au NPs compared to DMF alone and SMF with NGF-SPIO-Au NPs, respectively, which was attributed to successive membrane depolarization. Cellular uptake performed with the application of sodium azide proved that DMF enhanced cellular uptake of NGF-SPIO-Au NPs via endocytosis. In addition, DMF upregulated both the neural differentiation marker (β3-tubulin) and the cell adhesive molecule (integrin-β1) with the existence of NGF-SPIO-Au NPs, while SMF did not show these effects. The results imply that noninvasive DMF-stimulated NPs can regulate intracellular Ca2+ influx and enhance neuron differentiation and neuroregeneration rate.http://www.sciencedirect.com/science/article/pii/S2452199X23000075Calcium influxMembrane potentialDynamic magnetic fieldsNeuroregenerationSPIO-Au Core-shell nanoparticles |
| spellingShingle | Elias Georgas Muzhaozi Yuan Jingfan Chen Ya Wang Yi-Xian Qin Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca2+ influx and differentiation Bioactive Materials Calcium influx Membrane potential Dynamic magnetic fields Neuroregeneration SPIO-Au Core-shell nanoparticles |
| title | Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca2+ influx and differentiation |
| title_full | Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca2+ influx and differentiation |
| title_fullStr | Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca2+ influx and differentiation |
| title_full_unstemmed | Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca2+ influx and differentiation |
| title_short | Bioactive superparamagnetic iron oxide-gold nanoparticles regulated by a dynamic magnetic field induce neuronal Ca2+ influx and differentiation |
| title_sort | bioactive superparamagnetic iron oxide gold nanoparticles regulated by a dynamic magnetic field induce neuronal ca2 influx and differentiation |
| topic | Calcium influx Membrane potential Dynamic magnetic fields Neuroregeneration SPIO-Au Core-shell nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23000075 |
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