Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China
Although limited progress have been made about pathogen system of Hibiscus rosa-sinensis and Hibiscus latent Fort Pierce virus (HLFPV), interaction between plant host and pathogen remain largely unknown, which led to deficiency of effective measures to control disease of hibiscus plants caused by HL...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2020-12-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721542 |
| _version_ | 1818331772060958720 |
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| author | Han-hong Lan Luan-mei Lu |
| author_facet | Han-hong Lan Luan-mei Lu |
| author_sort | Han-hong Lan |
| collection | DOAJ |
| description | Although limited progress have been made about pathogen system of Hibiscus rosa-sinensis and Hibiscus latent Fort Pierce virus (HLFPV), interaction between plant host and pathogen remain largely unknown, which led to deficiency of effective measures to control disease of hibiscus plants caused by HLFPV. In this study, infection of HLFPV in Hibiscus rosa-sinensis was firstly confirmed for the first time by traditional electron microscopy, modern reverse transcription polymerase chain reaction and RNA-seq methods in China (HLFPV-Ch). Sequence properties analyzing suggested that the full-length sequences (6,465 nt) of HLFPV-Ch had a high sequence identity and a similar genomic structure with other tobamoviruses. It includes a 5′-terminal untranslated region (UTR), followed by four open reading frames encoding for a 128.5-kDa replicase, a 186.5-kDa polymerase, a 31-kDa movement protein, 17.6-kDa coat protein, and the last a 3′-terminal UTR. Furthermore, HLFPV-Ch–derived virus-derived siRNAs (vsiRNAs) ant its putative target genes, reported also for the first time, were identified and characterized from disease Hibiscus rosa-sinensis through sRNAseq and Patmatch server to investigate the interaction in this pathogen systems. HLFPV-Ch–derived vsiRNAs demonstrated several general and specific characteristics. Gene Ontology classification revealed predicted target genes by vsiRNAs are involved in abroad range of cellular component, molecular function and biological processes. Taken together, for first time, our results certified the HLFPV infection in China and provide an insight into interaction between HLFPV and Hibiscus rosa-sinensis. |
| first_indexed | 2024-12-13T13:25:09Z |
| format | Article |
| id | doaj.art-7b562d66094b40ada73243665a9d3d15 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-13T13:25:09Z |
| publishDate | 2020-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-7b562d66094b40ada73243665a9d3d152022-12-21T23:44:18ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542020-12-0136661862710.5423/PPJ.OA.09.2020.016910.5423PPJ.OA.09.2020.0169Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in ChinaHan-hong Lan0Luan-mei Lu1Key Laboratory of Landscape Plants with Fujian and Taiwan Characteristics of Fujian Colleges and Universities, School of Biological Sciences and Biotechnology, Minnan Normal University, Zhangzhou 363000, ChinaKey Laboratory of Landscape Plants with Fujian and Taiwan Characteristics of Fujian Colleges and Universities, School of Biological Sciences and Biotechnology, Minnan Normal University, Zhangzhou 363000, ChinaAlthough limited progress have been made about pathogen system of Hibiscus rosa-sinensis and Hibiscus latent Fort Pierce virus (HLFPV), interaction between plant host and pathogen remain largely unknown, which led to deficiency of effective measures to control disease of hibiscus plants caused by HLFPV. In this study, infection of HLFPV in Hibiscus rosa-sinensis was firstly confirmed for the first time by traditional electron microscopy, modern reverse transcription polymerase chain reaction and RNA-seq methods in China (HLFPV-Ch). Sequence properties analyzing suggested that the full-length sequences (6,465 nt) of HLFPV-Ch had a high sequence identity and a similar genomic structure with other tobamoviruses. It includes a 5′-terminal untranslated region (UTR), followed by four open reading frames encoding for a 128.5-kDa replicase, a 186.5-kDa polymerase, a 31-kDa movement protein, 17.6-kDa coat protein, and the last a 3′-terminal UTR. Furthermore, HLFPV-Ch–derived virus-derived siRNAs (vsiRNAs) ant its putative target genes, reported also for the first time, were identified and characterized from disease Hibiscus rosa-sinensis through sRNAseq and Patmatch server to investigate the interaction in this pathogen systems. HLFPV-Ch–derived vsiRNAs demonstrated several general and specific characteristics. Gene Ontology classification revealed predicted target genes by vsiRNAs are involved in abroad range of cellular component, molecular function and biological processes. Taken together, for first time, our results certified the HLFPV infection in China and provide an insight into interaction between HLFPV and Hibiscus rosa-sinensis.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721542hibiscus latent fort pierce virushibiscus rosa-sinensisinteractionsrna-seqtarget genes |
| spellingShingle | Han-hong Lan Luan-mei Lu Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China The Plant Pathology Journal hibiscus latent fort pierce virus hibiscus rosa-sinensis interaction srna-seq target genes |
| title | Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China |
| title_full | Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China |
| title_fullStr | Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China |
| title_full_unstemmed | Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China |
| title_short | Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China |
| title_sort | characterization of hibiscus latent fort pierce virus derived sirnas in infected hibiscus rosa sinensis in china |
| topic | hibiscus latent fort pierce virus hibiscus rosa-sinensis interaction srna-seq target genes |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721542 |
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