Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum
In this study, the transcriptional profiling of Penicillium digitatum after C12O3TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KE...
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.574882/full |
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| author | Xindan Li Guirong Feng Wenjun Wang Lanhua Yi Lanhua Yi Lili Deng Lili Deng Kaifang Zeng Kaifang Zeng |
| author_facet | Xindan Li Guirong Feng Wenjun Wang Lanhua Yi Lanhua Yi Lili Deng Lili Deng Kaifang Zeng Kaifang Zeng |
| author_sort | Xindan Li |
| collection | DOAJ |
| description | In this study, the transcriptional profiling of Penicillium digitatum after C12O3TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C12O3TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of β-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C12O3TR could damage the cell wall integrity. In conclusion, these results suggested that C12O3TR could inhibit the growth of P. digitatum through various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity. |
| first_indexed | 2024-12-11T06:05:14Z |
| format | Article |
| id | doaj.art-7b56fb382f9949d8baebc59d1272e3b2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T06:05:14Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7b56fb382f9949d8baebc59d1272e3b22022-12-22T01:18:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-09-011110.3389/fmicb.2020.574882574882Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatumXindan Li0Guirong Feng1Wenjun Wang2Lanhua Yi3Lanhua Yi4Lili Deng5Lili Deng6Kaifang Zeng7Kaifang Zeng8College of Food Science, Southwest University, Chongqing, ChinaCollege of Food Science, Southwest University, Chongqing, ChinaCollege of Food Science, Southwest University, Chongqing, ChinaCollege of Food Science, Southwest University, Chongqing, ChinaResearch Center of Food Storage & Logistics, Southwest University, Chongqing, ChinaCollege of Food Science, Southwest University, Chongqing, ChinaResearch Center of Food Storage & Logistics, Southwest University, Chongqing, ChinaCollege of Food Science, Southwest University, Chongqing, ChinaResearch Center of Food Storage & Logistics, Southwest University, Chongqing, ChinaIn this study, the transcriptional profiling of Penicillium digitatum after C12O3TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C12O3TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of β-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C12O3TR could damage the cell wall integrity. In conclusion, these results suggested that C12O3TR could inhibit the growth of P. digitatum through various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity.https://www.frontiersin.org/article/10.3389/fmicb.2020.574882/fullPenicillium digitatumpeptideC12O3TRRNA-Seqcell wall |
| spellingShingle | Xindan Li Guirong Feng Wenjun Wang Lanhua Yi Lanhua Yi Lili Deng Lili Deng Kaifang Zeng Kaifang Zeng Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum Frontiers in Microbiology Penicillium digitatum peptide C12O3TR RNA-Seq cell wall |
| title | Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
| title_full | Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
| title_fullStr | Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
| title_full_unstemmed | Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
| title_short | Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum |
| title_sort | effects of peptide c12 ooww nh2 on transcriptome and cell wall of the postharvest fungal pathogen penicillium digitatum |
| topic | Penicillium digitatum peptide C12O3TR RNA-Seq cell wall |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.574882/full |
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