Data transformation in biological assays

The analysis of variance is the statistical test most used for comparison of three or more means simultaneously. Its application requires, however, the compliance to some assumptions, with main emphasis on normality of the data and homoscedasticity of variances. When such requirements are not met, o...

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Main Authors: Sylvio Péllico Netto, Alexandre Behling
Format: Article
Language:English
Published: Embrapa Florestas 2021-03-01
Series:Pesquisa Florestal Brasileira
Subjects:
Online Access:https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1916
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author Sylvio Péllico Netto
Alexandre Behling
author_facet Sylvio Péllico Netto
Alexandre Behling
author_sort Sylvio Péllico Netto
collection DOAJ
description The analysis of variance is the statistical test most used for comparison of three or more means simultaneously. Its application requires, however, the compliance to some assumptions, with main emphasis on normality of the data and homoscedasticity of variances. When such requirements are not met, one of the alternatives is the data transformation to enable the continuity of the experimental evaluation. With the proposition of the Tukey’s data transformation system, understood as a power transformation system, i.e. the application of nth root on a data set (X⅟n) this statistical procedure has methodologically evolved to ensure such solutions. In the present research we proposed a complement to this system, denominated here as transformation in four steps, with inclusion of two hypothesis tests to evaluate normality and homoscedasticity. This was applied on experimental data to evaluate the amount of radiation available at soil level within stands of Acacia mearnsii De Wild. We have proposed a model for data transformation to simultaneously obtain homoscedasticity and normality. The methodology was appropriate to ensure these two statistical aspects on the experimental data, allowing comparison of eight treatments by conventional analysis of variance. Index terms: Analysis of variance, homoscedasticity, normality.
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spelling doaj.art-7b598bc316a7422b9c585dbd8fceb9212022-12-21T17:23:55ZengEmbrapa FlorestasPesquisa Florestal Brasileira1809-36471983-26052021-03-014110.4336/2021.pfb.41e201901916Data transformation in biological assaysSylvio Péllico Netto0Alexandre Behling1Universidade Federal do Paraná, Departamento de Ciências FlorestaisUniversidade Federal do Paraná, Departamento de Ciências FlorestaisThe analysis of variance is the statistical test most used for comparison of three or more means simultaneously. Its application requires, however, the compliance to some assumptions, with main emphasis on normality of the data and homoscedasticity of variances. When such requirements are not met, one of the alternatives is the data transformation to enable the continuity of the experimental evaluation. With the proposition of the Tukey’s data transformation system, understood as a power transformation system, i.e. the application of nth root on a data set (X⅟n) this statistical procedure has methodologically evolved to ensure such solutions. In the present research we proposed a complement to this system, denominated here as transformation in four steps, with inclusion of two hypothesis tests to evaluate normality and homoscedasticity. This was applied on experimental data to evaluate the amount of radiation available at soil level within stands of Acacia mearnsii De Wild. We have proposed a model for data transformation to simultaneously obtain homoscedasticity and normality. The methodology was appropriate to ensure these two statistical aspects on the experimental data, allowing comparison of eight treatments by conventional analysis of variance. Index terms: Analysis of variance, homoscedasticity, normality.https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1916Analysis of varianceAcacia mearnsiiRadiation
spellingShingle Sylvio Péllico Netto
Alexandre Behling
Data transformation in biological assays
Pesquisa Florestal Brasileira
Analysis of variance
Acacia mearnsii
Radiation
title Data transformation in biological assays
title_full Data transformation in biological assays
title_fullStr Data transformation in biological assays
title_full_unstemmed Data transformation in biological assays
title_short Data transformation in biological assays
title_sort data transformation in biological assays
topic Analysis of variance
Acacia mearnsii
Radiation
url https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1916
work_keys_str_mv AT sylviopelliconetto datatransformationinbiologicalassays
AT alexandrebehling datatransformationinbiologicalassays