THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN
Abstract. Objectives: Chronic viral hepatitis В (CHB) and С (CHC) comprise an important issue of modern pediatrics. Immunogenetic disorders are among key factors of their pathogenesis. The aim of present research was to reveal interactions between polymorphisms of cytokine IL-1β and IL-6 genes and c...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
|
| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/672 |
| _version_ | 1797198026357342208 |
|---|---|
| author | S. V. Romanova Т. А. Vidmanova Е. А. Zhukova Е. V. Ermolina N. I. Tolkacheva I. V. Mayanskaya I. А. Timchenko |
| author_facet | S. V. Romanova Т. А. Vidmanova Е. А. Zhukova Е. V. Ermolina N. I. Tolkacheva I. V. Mayanskaya I. А. Timchenko |
| author_sort | S. V. Romanova |
| collection | DOAJ |
| description | Abstract. Objectives: Chronic viral hepatitis В (CHB) and С (CHC) comprise an important issue of modern pediatrics. Immunogenetic disorders are among key factors of their pathogenesis. The aim of present research was to reveal interactions between polymorphisms of cytokine IL-1β and IL-6 genes and clinical characteristics of the disease in children.The study included forty-eight children aged from 6 to 17 years (a median of 12 years old), including thirty patients with CHC, and eighteen – with CHB. Polymorphism of cytokine genes – interleukin 1β (IL-1β) – T31C, interleukin 6 (IL-6) – G174C was evaluated by means of allele-specific polymerase chain reaction (PCR); the amplification products were detected by horizontal electrophoresis (“Litekh”, Russia).The groups of CHB and CHC patients were characterized by increased prevalence of CC genotype of IL- 1β Т/С -31gene and СС genotype of IL-6 -174 G/C gene variants, as compared to control group [OR = 3.66 (95% CI – 1.2-11.0) and OR = 2.81 (95% CI – 1.33-5.87)]. The IL-6 -174 G/C polymorphism of is associated with higher IL-1β synthesis in GG genotype carriers, when compared to GC (p = 0.016) and СС genotypes (p = 0.030). In children with active CHB and CHC, polymorphisms of IL-1β -31 ТС and СС, as well as IL-6 -174 СC, were related to the higher cytolysis level, while the genotypes IL-1β -31 ТТ and IL-6 -174 GC and GG – with absence of cytolytic features(p = 0.022; p = 0.038).Presence of polymorphisms of IL-1β and IL-6 genes may sufficiently contribute to susceptibility to chronic hepatitis development and have an impact on extent of clinical and immunological presentations of the disease. |
| first_indexed | 2024-03-08T05:49:47Z |
| format | Article |
| id | doaj.art-7b59fec2400b4a1bbf85cbca3af07e45 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:53:19Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-7b59fec2400b4a1bbf85cbca3af07e452024-04-22T13:07:39ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0115653554210.15789/1563-0625-2013-6-535-542669THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDRENS. V. Romanova0Т. А. Vidmanova1Е. А. Zhukova2Е. V. Ermolina3N. I. Tolkacheva4I. V. Mayanskaya5I. А. Timchenko6Nizhny Novgorod Research Institute of Children Gastroenterology, Ministry of Health of the Russian Federation, Nizhny NovgorodNizhny Novgorod Research Institute of Children Gastroenterology, Ministry of Health of the Russian Federation, Nizhny NovgorodNizhny Novgorod Research Institute of Children Gastroenterology, Ministry of Health of the Russian Federation, Nizhny NovgorodNizhny Novgorod Research Institute of Children Gastroenterology, Ministry of Health of the Russian Federation, Nizhny NovgorodNizhny Novgorod Research Institute of Children Gastroenterology, Ministry of Health of the Russian Federation, Nizhny NovgorodNizhny Novgorod Research Institute of Children Gastroenterology, Ministry of Health of the Russian Federation, Nizhny NovgorodNizhny Novgorod Research Institute of Children Gastroenterology, Ministry of Health of the Russian Federation, Nizhny NovgorodAbstract. Objectives: Chronic viral hepatitis В (CHB) and С (CHC) comprise an important issue of modern pediatrics. Immunogenetic disorders are among key factors of their pathogenesis. The aim of present research was to reveal interactions between polymorphisms of cytokine IL-1β and IL-6 genes and clinical characteristics of the disease in children.The study included forty-eight children aged from 6 to 17 years (a median of 12 years old), including thirty patients with CHC, and eighteen – with CHB. Polymorphism of cytokine genes – interleukin 1β (IL-1β) – T31C, interleukin 6 (IL-6) – G174C was evaluated by means of allele-specific polymerase chain reaction (PCR); the amplification products were detected by horizontal electrophoresis (“Litekh”, Russia).The groups of CHB and CHC patients were characterized by increased prevalence of CC genotype of IL- 1β Т/С -31gene and СС genotype of IL-6 -174 G/C gene variants, as compared to control group [OR = 3.66 (95% CI – 1.2-11.0) and OR = 2.81 (95% CI – 1.33-5.87)]. The IL-6 -174 G/C polymorphism of is associated with higher IL-1β synthesis in GG genotype carriers, when compared to GC (p = 0.016) and СС genotypes (p = 0.030). In children with active CHB and CHC, polymorphisms of IL-1β -31 ТС and СС, as well as IL-6 -174 СC, were related to the higher cytolysis level, while the genotypes IL-1β -31 ТТ and IL-6 -174 GC and GG – with absence of cytolytic features(p = 0.022; p = 0.038).Presence of polymorphisms of IL-1β and IL-6 genes may sufficiently contribute to susceptibility to chronic hepatitis development and have an impact on extent of clinical and immunological presentations of the disease.https://www.mimmun.ru/mimmun/article/view/672chronic viral hepatitisinterleukinsgene polymorphismil-1βil-6children |
| spellingShingle | S. V. Romanova Т. А. Vidmanova Е. А. Zhukova Е. V. Ermolina N. I. Tolkacheva I. V. Mayanskaya I. А. Timchenko THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN Медицинская иммунология chronic viral hepatitis interleukins gene polymorphism il-1β il-6 children |
| title | THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_full | THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_fullStr | THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_full_unstemmed | THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_short | THE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDREN |
| title_sort | role of genetic polymorphisms of gene il 1β 31 т с and il 6 174 g c in the course of chronic hepatitis b and c in children |
| topic | chronic viral hepatitis interleukins gene polymorphism il-1β il-6 children |
| url | https://www.mimmun.ru/mimmun/article/view/672 |
| work_keys_str_mv | AT svromanova theroleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT tavidmanova theroleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT eazhukova theroleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT evermolina theroleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT nitolkacheva theroleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT ivmayanskaya theroleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT iatimchenko theroleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT svromanova roleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT tavidmanova roleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT eazhukova roleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT evermolina roleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT nitolkacheva roleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT ivmayanskaya roleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren AT iatimchenko roleofgeneticpolymorphismsofgeneil1b31tsandil6174gcinthecourseofchronichepatitisbandcinchildren |