PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp
This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast quality leaf mesophyll Dendrobium sp. Protoplast can be used for culture protoplast, for somatic cross, biology research, and material for genetic manipulation. This research to ma...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indonesian Biological Society
2014-01-01
|
| Series: | Journal of Biological Researches |
| Subjects: | |
| Online Access: | http://berkalahayati.org/index.php/jurnal/article/view/174 |
| _version_ | 1819207302197018624 |
|---|---|
| author | Edu Setiti Wida Utami |
| author_facet | Edu Setiti Wida Utami |
| author_sort | Edu Setiti Wida Utami |
| collection | DOAJ |
| description | This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast quality leaf mesophyll Dendrobium sp. Protoplast can be used for culture protoplast, for somatic cross, biology research, and material for genetic manipulation. This research to make use of material was Dendrobium sp Orchid. Explant that to used was leaf mesophyll Dendrobium sp. For the protoplast isolation to used enzyme combination cellulase and maserozym (Onozuka R-10). Yakult JONSHA Co., Ltd., (Japan) with concentration cellulase : maserozym is (0.5; 0.05; 0.75; 0.075; 0; 0.1). leaf mesophyll Dendrobium sp. to get a soak in enzyme solution with incubation time 12-13 hours and 14-1 hours.the result shown that enzyme combination selulase and maserozym can be used for protoplast isolation leaf mesophyll Dendrobium sp Orchid. The best enzyme concentration to fit for isolation protoplast leaf mesophyll Dendrobium sp to be selulase 0.75% and maserozym 0.075% with incubation time 14-15 hours. |
| first_indexed | 2024-12-23T05:21:20Z |
| format | Article |
| id | doaj.art-7b5adb1de9d34baa889d359e4908ab46 |
| institution | Directory Open Access Journal |
| issn | 0852-6834 2337-389X |
| language | English |
| last_indexed | 2024-12-23T05:21:20Z |
| publishDate | 2014-01-01 |
| publisher | Indonesian Biological Society |
| record_format | Article |
| series | Journal of Biological Researches |
| spelling | doaj.art-7b5adb1de9d34baa889d359e4908ab462022-12-21T17:58:42ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2014-01-01126367174PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium SpEdu Setiti Wida Utami0Jurusan Biologi FMIPA Universitas Airlangga Surabaya 60115This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast quality leaf mesophyll Dendrobium sp. Protoplast can be used for culture protoplast, for somatic cross, biology research, and material for genetic manipulation. This research to make use of material was Dendrobium sp Orchid. Explant that to used was leaf mesophyll Dendrobium sp. For the protoplast isolation to used enzyme combination cellulase and maserozym (Onozuka R-10). Yakult JONSHA Co., Ltd., (Japan) with concentration cellulase : maserozym is (0.5; 0.05; 0.75; 0.075; 0; 0.1). leaf mesophyll Dendrobium sp. to get a soak in enzyme solution with incubation time 12-13 hours and 14-1 hours.the result shown that enzyme combination selulase and maserozym can be used for protoplast isolation leaf mesophyll Dendrobium sp Orchid. The best enzyme concentration to fit for isolation protoplast leaf mesophyll Dendrobium sp to be selulase 0.75% and maserozym 0.075% with incubation time 14-15 hours.http://berkalahayati.org/index.php/jurnal/article/view/174protoplast isolationenzimincubation time |
| spellingShingle | Edu Setiti Wida Utami PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp Journal of Biological Researches protoplast isolation enzim incubation time |
| title | PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp |
| title_full | PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp |
| title_fullStr | PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp |
| title_full_unstemmed | PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp |
| title_short | PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp |
| title_sort | pengaruh penambahan enzim dan waktu inkubasi terhadap jumlah protoplas mesofil daun anggrek dendrobium sp |
| topic | protoplast isolation enzim incubation time |
| url | http://berkalahayati.org/index.php/jurnal/article/view/174 |
| work_keys_str_mv | AT edusetitiwidautami pengaruhpenambahanenzimdanwaktuinkubasiterhadapjumlahprotoplasmesofildaunanggrekdendrobiumsp |