Fisetin-metformin co-loaded in mesoporous silica nanoparticles (MSNs) inhibited triple negative breast cancer proliferation
Objective(s): Herbal compounds with cytotoxicity have been of great interest in recent years to improve cancer treatment methods. Fisetin is an anti-cancer herbal compound with low solubility in aqueous systems. Metformin is another compound with anti-cancer effects. In this study, the combined effe...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2023-10-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_23121_9d77ebed4a2c02f10018e0bcc5d4e557.pdf |
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| author | Mohammad Reza Karimzadeh Mahshid Samie Ghahfarokhi Razieh Heidari Somayeh Reiisi |
| author_facet | Mohammad Reza Karimzadeh Mahshid Samie Ghahfarokhi Razieh Heidari Somayeh Reiisi |
| author_sort | Mohammad Reza Karimzadeh |
| collection | DOAJ |
| description | Objective(s): Herbal compounds with cytotoxicity have been of great interest in recent years to improve cancer treatment methods. Fisetin is an anti-cancer herbal compound with low solubility in aqueous systems. Metformin is another compound with anti-cancer effects. In this study, the combined effect of fisetin and metformin was investigated using mesoporous silica nanoparticles (MSNs) in breast cancer cell lines. Materials and Methods: After the synthesis of nanoparticles, they were characterized using XRD, TEM, SEM. The DLS test showed a size of 143.4 nm with zeta-potential -39.1 mV. Fisetin and metformin were loaded into nanoparticles and loading was confirmed by FTIR. The toxicity of different concentrations of free drug (metformin, fisetin, fisetin-metformin) and Nanoformulations (metformin, fisetin and nano-fisetin-metformin) was investigated on two breast cancer lines MCF7 and MDA-MB-231. Results: Fisetin-metformin co-loaded in MSNs showed the highest cytotoxicity among all formulations in both cell lines. The inhibition of colony formation and migration rate was effectively observed in the co-treatment of cells with fisetin and metformin loaded in nanoparticles compared to single treatments. The expression of tumor suppressor miR-200b-3p and miR-34a-5p showed that fisetin increased the expression of these tumor suppressors compared to the control. Conclusion: The anti-cancer effect of fisetin-metformin in combination increased the expression of tumor suppressors due to the regulation of a wide range of gene network involving in cancer progress. The obtained results highlight the use of MSN as an effective drug delivery system for simultaneous delivery of herbal cytotoxic compounds in cancer. |
| first_indexed | 2024-03-09T07:15:36Z |
| format | Article |
| id | doaj.art-7b5f2bff9a194f20a6ea7f83868991e3 |
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| issn | 2322-3049 2322-5904 |
| language | English |
| last_indexed | 2024-03-09T07:15:36Z |
| publishDate | 2023-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj.art-7b5f2bff9a194f20a6ea7f83868991e32023-12-03T08:35:41ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042023-10-0110429330310.22038/nmj.2023.73702.179623121Fisetin-metformin co-loaded in mesoporous silica nanoparticles (MSNs) inhibited triple negative breast cancer proliferationMohammad Reza Karimzadeh0Mahshid Samie Ghahfarokhi1Razieh Heidari2Somayeh Reiisi3Department of Medical Genetics, School of Medicine, Bam University of Medical Sciences, Bam, IranDepartment of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, IranDepartment of Medical Biotechnology, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, IranDepartment of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, IranObjective(s): Herbal compounds with cytotoxicity have been of great interest in recent years to improve cancer treatment methods. Fisetin is an anti-cancer herbal compound with low solubility in aqueous systems. Metformin is another compound with anti-cancer effects. In this study, the combined effect of fisetin and metformin was investigated using mesoporous silica nanoparticles (MSNs) in breast cancer cell lines. Materials and Methods: After the synthesis of nanoparticles, they were characterized using XRD, TEM, SEM. The DLS test showed a size of 143.4 nm with zeta-potential -39.1 mV. Fisetin and metformin were loaded into nanoparticles and loading was confirmed by FTIR. The toxicity of different concentrations of free drug (metformin, fisetin, fisetin-metformin) and Nanoformulations (metformin, fisetin and nano-fisetin-metformin) was investigated on two breast cancer lines MCF7 and MDA-MB-231. Results: Fisetin-metformin co-loaded in MSNs showed the highest cytotoxicity among all formulations in both cell lines. The inhibition of colony formation and migration rate was effectively observed in the co-treatment of cells with fisetin and metformin loaded in nanoparticles compared to single treatments. The expression of tumor suppressor miR-200b-3p and miR-34a-5p showed that fisetin increased the expression of these tumor suppressors compared to the control. Conclusion: The anti-cancer effect of fisetin-metformin in combination increased the expression of tumor suppressors due to the regulation of a wide range of gene network involving in cancer progress. The obtained results highlight the use of MSN as an effective drug delivery system for simultaneous delivery of herbal cytotoxic compounds in cancer.https://nmj.mums.ac.ir/article_23121_9d77ebed4a2c02f10018e0bcc5d4e557.pdfbreast cancerdrug delivery systemsflavonolsmetforminnanoparticles |
| spellingShingle | Mohammad Reza Karimzadeh Mahshid Samie Ghahfarokhi Razieh Heidari Somayeh Reiisi Fisetin-metformin co-loaded in mesoporous silica nanoparticles (MSNs) inhibited triple negative breast cancer proliferation Nanomedicine Journal breast cancer drug delivery systems flavonols metformin nanoparticles |
| title | Fisetin-metformin co-loaded in mesoporous silica nanoparticles (MSNs) inhibited triple negative breast cancer proliferation |
| title_full | Fisetin-metformin co-loaded in mesoporous silica nanoparticles (MSNs) inhibited triple negative breast cancer proliferation |
| title_fullStr | Fisetin-metformin co-loaded in mesoporous silica nanoparticles (MSNs) inhibited triple negative breast cancer proliferation |
| title_full_unstemmed | Fisetin-metformin co-loaded in mesoporous silica nanoparticles (MSNs) inhibited triple negative breast cancer proliferation |
| title_short | Fisetin-metformin co-loaded in mesoporous silica nanoparticles (MSNs) inhibited triple negative breast cancer proliferation |
| title_sort | fisetin metformin co loaded in mesoporous silica nanoparticles msns inhibited triple negative breast cancer proliferation |
| topic | breast cancer drug delivery systems flavonols metformin nanoparticles |
| url | https://nmj.mums.ac.ir/article_23121_9d77ebed4a2c02f10018e0bcc5d4e557.pdf |
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