Comprehensive Analysis of the NF-YB Gene Family and Expression under Abiotic Stress and Hormone Treatment in <i>Larix kaempferi</i>
NF-YB, a subfamily of Nuclear Factor Y (NF-Y) transcription factor, play crucial role in many biological processes of plant growth and development and abiotic stress responses, and they can therefore be good candidate factors for breeding stress-resistant plants. However, the NF-YB proteins have not...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/10/8910 |
| Summary: | NF-YB, a subfamily of Nuclear Factor Y (NF-Y) transcription factor, play crucial role in many biological processes of plant growth and development and abiotic stress responses, and they can therefore be good candidate factors for breeding stress-resistant plants. However, the NF-YB proteins have not yet been explored in <i>Larix kaempferi</i>, a tree species with high economic and ecological values in northeast China and other regions, limiting the breeding of anti-stress <i>L. kaempferi.</i> In order to explore the roles of NF-YB transcription factors in <i>L. kaempferi</i>, we identified 20 <i>LkNF-YB</i> family genes from <i>L. kaempferi</i> full-length transcriptome data and carried out preliminary characterization of them through series of analyses on their phylogenetic relationships, conserved motif structure, subcellular localization prediction, GO annotation, promoter <i>cis</i>-acting elements as well as expression profiles under treatment of phytohormones (ABA, SA, MeJA) and abiotic stresses (salt and drought). The <i>LkNF-YB</i> genes were classified into three clades through phylogenetic analysis and belong to non-LEC1 type NF-YB transcription factors. They have 10 conserved motifs; all genes contain a common motif, and their promoters have various phytohormones and abiotic stress related <i>cis</i>-acting elements. Quantitative real time reverse transcription PCR (RT-qPCR) analysis showed that the sensitivity of the <i>LkNF-YB</i> genes to drought and salt stresses was higher in leaves than roots. The sensitivity of <i>LKNF-YB</i> genes to ABA, MeJA, SA stresses was much lower than that to abiotic stress. Among the <i>LkNF-YBs</i>, <i>LkNF-YB3</i> showed the strongest responses to drought and ABA treatments. Further protein interaction prediction analysis for <i>LkNF-YB3</i> revealed that <i>LkNF-YB3</i> interacts with various factors associated with stress responses and epigenetic regulation as well as NF-YA/NF-YC factors. Taken together, these results unveiled novel <i>L. kaempferi</i> NF-YB family genes and their characteristics, providing the basic knowledge for further in-depth studies on their roles in abiotic stress responses of <i>L. kaempferi</i>. |
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| ISSN: | 1661-6596 1422-0067 |