Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of <i>Stellaria media</i> (L.) Vill
The healing of skin wounds remains an important concern in medicine, especially in chronic wounds caused by various diseases such as diabetes. Using herbs or herbal products to heal skin wounds is a therapeutic challenge for traditional medicine. In this context, the main aim of our work was to high...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/23/11526 |
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| author | Florina Miere (Groza) Alin Cristian Teușdea Vasile Laslo Simona Cavalu Luminița Fritea Luciana Dobjanschi Mihaela Zdrinca Marcel Zdrinca Mariana Ganea Priscilla Pașc Adriana Ramona Memete Angela Antonescu Andreea Margareta Vlad Simona Ioana Vicas |
| author_facet | Florina Miere (Groza) Alin Cristian Teușdea Vasile Laslo Simona Cavalu Luminița Fritea Luciana Dobjanschi Mihaela Zdrinca Marcel Zdrinca Mariana Ganea Priscilla Pașc Adriana Ramona Memete Angela Antonescu Andreea Margareta Vlad Simona Ioana Vicas |
| author_sort | Florina Miere (Groza) |
| collection | DOAJ |
| description | The healing of skin wounds remains an important concern in medicine, especially in chronic wounds caused by various diseases such as diabetes. Using herbs or herbal products to heal skin wounds is a therapeutic challenge for traditional medicine. In this context, the main aim of our work was to highlight the in vitro healing potential of <i>Stellaria media</i> (L.) Vill. (SM) extract using the scratch assay on normal human dermal fibroblasts (NHDF). The ability to stimulate cell migration and proliferation under the influence of different concentrations of SM extract (range between 12.5 and 200 µg/mL) was determined compared to the control (untreated <i>in vitro</i>-simulated wound) and positive control (allantoin 50 µg/mL). Our results showed that the concentration of 100 µg/mL SM extract applied on the simulated wound recorded the strongest and fastest (24 h) migration (with wound closure) and proliferation of NHDF compared with the control. In addition, the SM extract was characterized in terms of bioactive compounds (total phenols and flavonoids content), antioxidant capacity (FRAP (The Ferric-Reducing Antioxidant Power) assay and electrochemical method), and antimicrobial activity. The results show that the SM extract contains a considerable amount of polyphenols (17.19 ± 1.32 mg GAE/g dw and 7.28 ± 1.18 mg QE/g dw for total phenol and flavonoid content, respectively) with antioxidant capacity. Antimicrobial activity against Gram-positive bacteria (<i>S. aureus</i>) is higher than <i>E. coli</i> at a dose of 15 µg/mL. This study showed that <i>Stellaria media</i> is a source of polyphenols compounds with antioxidant capacity, and for the first time, its wound healing potential was emphasized. |
| first_indexed | 2024-03-10T04:56:16Z |
| format | Article |
| id | doaj.art-7b61e9d601904a5aabebde00746f7e72 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:56:16Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-7b61e9d601904a5aabebde00746f7e722023-11-23T02:09:41ZengMDPI AGApplied Sciences2076-34172021-12-0111231152610.3390/app112311526Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of <i>Stellaria media</i> (L.) VillFlorina Miere (Groza)0Alin Cristian Teușdea1Vasile Laslo2Simona Cavalu3Luminița Fritea4Luciana Dobjanschi5Mihaela Zdrinca6Marcel Zdrinca7Mariana Ganea8Priscilla Pașc9Adriana Ramona Memete10Angela Antonescu11Andreea Margareta Vlad12Simona Ioana Vicas13Faculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Environmental Protection, University of Oradea, 26 Gen. Magheru Street, 410048 Oradea, RomaniaFaculty of Environmental Protection, University of Oradea, 26 Gen. Magheru Street, 410048 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaDoctoral School of Biomedical Science, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 10 P-ta 1 December Street, 410073 Oradea, RomaniaFaculty of Environmental Protection, University of Oradea, 26 Gen. Magheru Street, 410048 Oradea, RomaniaThe healing of skin wounds remains an important concern in medicine, especially in chronic wounds caused by various diseases such as diabetes. Using herbs or herbal products to heal skin wounds is a therapeutic challenge for traditional medicine. In this context, the main aim of our work was to highlight the in vitro healing potential of <i>Stellaria media</i> (L.) Vill. (SM) extract using the scratch assay on normal human dermal fibroblasts (NHDF). The ability to stimulate cell migration and proliferation under the influence of different concentrations of SM extract (range between 12.5 and 200 µg/mL) was determined compared to the control (untreated <i>in vitro</i>-simulated wound) and positive control (allantoin 50 µg/mL). Our results showed that the concentration of 100 µg/mL SM extract applied on the simulated wound recorded the strongest and fastest (24 h) migration (with wound closure) and proliferation of NHDF compared with the control. In addition, the SM extract was characterized in terms of bioactive compounds (total phenols and flavonoids content), antioxidant capacity (FRAP (The Ferric-Reducing Antioxidant Power) assay and electrochemical method), and antimicrobial activity. The results show that the SM extract contains a considerable amount of polyphenols (17.19 ± 1.32 mg GAE/g dw and 7.28 ± 1.18 mg QE/g dw for total phenol and flavonoid content, respectively) with antioxidant capacity. Antimicrobial activity against Gram-positive bacteria (<i>S. aureus</i>) is higher than <i>E. coli</i> at a dose of 15 µg/mL. This study showed that <i>Stellaria media</i> is a source of polyphenols compounds with antioxidant capacity, and for the first time, its wound healing potential was emphasized.https://www.mdpi.com/2076-3417/11/23/11526<i>Stellaria media</i> (L.) Villscratch assayfibroblastswound healingantioxidant capacityphenols and flavonoids content |
| spellingShingle | Florina Miere (Groza) Alin Cristian Teușdea Vasile Laslo Simona Cavalu Luminița Fritea Luciana Dobjanschi Mihaela Zdrinca Marcel Zdrinca Mariana Ganea Priscilla Pașc Adriana Ramona Memete Angela Antonescu Andreea Margareta Vlad Simona Ioana Vicas Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of <i>Stellaria media</i> (L.) Vill Applied Sciences <i>Stellaria media</i> (L.) Vill scratch assay fibroblasts wound healing antioxidant capacity phenols and flavonoids content |
| title | Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of <i>Stellaria media</i> (L.) Vill |
| title_full | Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of <i>Stellaria media</i> (L.) Vill |
| title_fullStr | Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of <i>Stellaria media</i> (L.) Vill |
| title_full_unstemmed | Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of <i>Stellaria media</i> (L.) Vill |
| title_short | Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of <i>Stellaria media</i> (L.) Vill |
| title_sort | evaluation of in vitro wound healing potential antioxidant capacity and antimicrobial activity of i stellaria media i l vill |
| topic | <i>Stellaria media</i> (L.) Vill scratch assay fibroblasts wound healing antioxidant capacity phenols and flavonoids content |
| url | https://www.mdpi.com/2076-3417/11/23/11526 |
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