Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling
Defensin genes form part of a plant’s defense system and are activated when exposed to biotic or abiotic stress. They play a vital role in controlling many signaling pathways involved in various plant defense mechanisms. This research aimed to isolate and characterize novel defensin genes from selec...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.975852/full |
| _version_ | 1818474788264345600 |
|---|---|
| author | Raheela Jabeen Atia Iqbal Farah Deeba Faisal Zulfiqar Ghulam Mustafa Haq Nawaz Ume Habiba Muhammad Nafees Abbu Zaid Kadambot H. M. Siddique |
| author_facet | Raheela Jabeen Atia Iqbal Farah Deeba Faisal Zulfiqar Ghulam Mustafa Haq Nawaz Ume Habiba Muhammad Nafees Abbu Zaid Kadambot H. M. Siddique |
| author_sort | Raheela Jabeen |
| collection | DOAJ |
| description | Defensin genes form part of a plant’s defense system and are activated when exposed to biotic or abiotic stress. They play a vital role in controlling many signaling pathways involved in various plant defense mechanisms. This research aimed to isolate and characterize novel defensin genes from selected medicinally important plants to explore their signaling mechanisms and defense associated roles for breeding. The DNA of Albizia lebbeck and Moringa oleifera was subjected to PCR amplification using gene-specific primers of defensin genes. Two novel defensin genes were isolated in each species, with sequence lengths of 300 bp in A. lebbeck and 150 bp in M. oleifera. In-silico analysis undertaken to retrieve and align their orthologous sequences revealed 100% similarity of the A. lebbeck gene with the Musa acuminate peroxidase P7-like gene and 85% similarity of the M. oleifera gene with the Manihot esculenta GDP dissociation inhibitor gene. The reliability, stability and physiochemical properties of homology models of these sequences was confirmed through online computational studies. This preliminary study confirmed the presence of novel genes with peroxidase P7 and Rab GDP dissociation inhibitor gene-like activity in A. lebbeck and M. oleifera, respectively, and their potential defense role in plants. Thus, the defensin genes of both species could be used in the synthesis of transgenic self-defensive plants with increased disease resistance and as potential candidates for improved crop production and thraputic formulation in the future. |
| first_indexed | 2024-04-14T04:40:56Z |
| format | Article |
| id | doaj.art-7b62d3b8085c4f8e978994ea5744a2d4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T04:40:56Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7b62d3b8085c4f8e978994ea5744a2d42022-12-22T02:11:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.975852975852Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signalingRaheela Jabeen0Atia Iqbal1Farah Deeba2Faisal Zulfiqar3Ghulam Mustafa4Haq Nawaz5Ume Habiba6Muhammad Nafees7Abbu Zaid8Kadambot H. M. Siddique9Department of Biochemistry and Biotechnology, The Women University Multan, Multan, Punjab, PakistanDepartment of Microbiology and Molecular Genetics, The Women University Multan, Multan, Punjab, PakistanDepartment of Biochemistry and Biotechnology, The Women University Multan, Multan, Punjab, PakistanDepartment of Horticultural Sciences, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, PakistanDepartment of Biochemsitry, Government College University Faisalabad, Faisalabad, Punjab, PakistanDepartment of Biochemistry, Bahauddin Zakariya University, Multan, Punjab, PakistanDepartment of Microbiology and Molecular Genetics, The Women University Multan, Multan, Punjab, PakistanDepartment of Horticultural Sciences, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, PakistanAligarh Muslim University, Aligarh, Uttar Pradesh, IndiaUniversity of Western Australia, Perth, WA, AustraliaDefensin genes form part of a plant’s defense system and are activated when exposed to biotic or abiotic stress. They play a vital role in controlling many signaling pathways involved in various plant defense mechanisms. This research aimed to isolate and characterize novel defensin genes from selected medicinally important plants to explore their signaling mechanisms and defense associated roles for breeding. The DNA of Albizia lebbeck and Moringa oleifera was subjected to PCR amplification using gene-specific primers of defensin genes. Two novel defensin genes were isolated in each species, with sequence lengths of 300 bp in A. lebbeck and 150 bp in M. oleifera. In-silico analysis undertaken to retrieve and align their orthologous sequences revealed 100% similarity of the A. lebbeck gene with the Musa acuminate peroxidase P7-like gene and 85% similarity of the M. oleifera gene with the Manihot esculenta GDP dissociation inhibitor gene. The reliability, stability and physiochemical properties of homology models of these sequences was confirmed through online computational studies. This preliminary study confirmed the presence of novel genes with peroxidase P7 and Rab GDP dissociation inhibitor gene-like activity in A. lebbeck and M. oleifera, respectively, and their potential defense role in plants. Thus, the defensin genes of both species could be used in the synthesis of transgenic self-defensive plants with increased disease resistance and as potential candidates for improved crop production and thraputic formulation in the future.https://www.frontiersin.org/articles/10.3389/fpls.2022.975852/fulldefensin geneAlbizia lebbeckMoringa oleiferain silico analysisplant defense system |
| spellingShingle | Raheela Jabeen Atia Iqbal Farah Deeba Faisal Zulfiqar Ghulam Mustafa Haq Nawaz Ume Habiba Muhammad Nafees Abbu Zaid Kadambot H. M. Siddique Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling Frontiers in Plant Science defensin gene Albizia lebbeck Moringa oleifera in silico analysis plant defense system |
| title | Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling |
| title_full | Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling |
| title_fullStr | Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling |
| title_full_unstemmed | Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling |
| title_short | Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling |
| title_sort | isolation and characterization of peroxidase p7 like gene and rab gdi like gene from potential medicinal plants a step toward understanding cell defense signaling |
| topic | defensin gene Albizia lebbeck Moringa oleifera in silico analysis plant defense system |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.975852/full |
| work_keys_str_mv | AT raheelajabeen isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT atiaiqbal isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT farahdeeba isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT faisalzulfiqar isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT ghulammustafa isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT haqnawaz isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT umehabiba isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT muhammadnafees isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT abbuzaid isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling AT kadambothmsiddique isolationandcharacterizationofperoxidasep7likegeneandrabgdilikegenefrompotentialmedicinalplantsasteptowardunderstandingcelldefensesignaling |