Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich <i>Cordyceps militaris</i> Polysaccharides on the High-Fat Diet-Fed Mice Model
Supplementation of polysaccharides is a promising gut microbiota-targeted therapeutic method for obesity and metabolic diseases. Biological activities of <i>Cordyceps militaris</i> polysaccharides have been well reported, but the effect of selenium (Se)-rich <i>C. militaris</i&g...
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MDPI AG
2021-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/10/2252 |
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| author | Minglei Yu Jin Yue Nan Hui Yuee Zhi Kashif Hayat Xijia Yang Dan Zhang Shaohua Chu Pei Zhou |
| author_facet | Minglei Yu Jin Yue Nan Hui Yuee Zhi Kashif Hayat Xijia Yang Dan Zhang Shaohua Chu Pei Zhou |
| author_sort | Minglei Yu |
| collection | DOAJ |
| description | Supplementation of polysaccharides is a promising gut microbiota-targeted therapeutic method for obesity and metabolic diseases. Biological activities of <i>Cordyceps militaris</i> polysaccharides have been well reported, but the effect of selenium (Se)-rich <i>C. militaris</i> polysaccharides (SeCMP) on obesity and associated metabolic disorder and gut microbiota composition has been rarely studied. This study aimed to investigate the anti-obesity and gut microbiota modulatory effect of crude polysaccharides separated from Se-rich <i>C. militaris</i> on a high-fat diet (HFD)-fed C57BL/6J mice model. Mice were treated with a normal diet (CHOW), HFD alone, HFD plus <i>C. militaris</i> polysaccharides (CMP), or low/medium/high dosage of SeCMP for 8 weeks. Body weight, fat content, serum lipid, appetite hormone, lipid gene expression, inflammation cytokines, thermogenic protein, short-chain fatty acids (SCFAs), and gut microbiota structure of the mice were determined. Compared with HFD-fed mice, the serum triglyceride and low-density lipoprotein cholesterol (LDL-C) in the SeCMP-200 group were decreased by 51.5% and 44.1%, respectively. Furthermore, serum lipopolysaccharide-binding proteins (LBP), adiponectin level, and pro-inflammation gene expression in the colon and subcutaneous fat were inhibited, whereas anti-inflammation gene expression was improved, reflecting SeCMP-200 might mitigate obese-induced inflammation. Meanwhile, SeCMP-200 promoted satiety and thermogenesis of obese mice. It also significantly decreased gut bacteria, such as <i>Dorea</i>, <i>Lactobacillus</i>, <i>Clostridium</i>, <i>Ruminococcus,</i> that negatively correlated with obesity traits and increased mucosal beneficial bacteria <i>Akkermansia</i>. There was no significant difference between CMP and SeCMP-100 groups. Our results revealed a high dose of SeCMP could prevent HFD-induced dyslipidemia and gut microbiota dysbiosis and was potential to be used as functional foods. |
| first_indexed | 2024-03-10T06:34:45Z |
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| language | English |
| last_indexed | 2024-03-10T06:34:45Z |
| publishDate | 2021-09-01 |
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| spelling | doaj.art-7b66f09dc6d4448fb8bb7f1edfb9bfff2023-11-22T18:13:07ZengMDPI AGFoods2304-81582021-09-011010225210.3390/foods10102252Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich <i>Cordyceps militaris</i> Polysaccharides on the High-Fat Diet-Fed Mice ModelMinglei Yu0Jin Yue1Nan Hui2Yuee Zhi3Kashif Hayat4Xijia Yang5Dan Zhang6Shaohua Chu7Pei Zhou8School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaBor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSupplementation of polysaccharides is a promising gut microbiota-targeted therapeutic method for obesity and metabolic diseases. Biological activities of <i>Cordyceps militaris</i> polysaccharides have been well reported, but the effect of selenium (Se)-rich <i>C. militaris</i> polysaccharides (SeCMP) on obesity and associated metabolic disorder and gut microbiota composition has been rarely studied. This study aimed to investigate the anti-obesity and gut microbiota modulatory effect of crude polysaccharides separated from Se-rich <i>C. militaris</i> on a high-fat diet (HFD)-fed C57BL/6J mice model. Mice were treated with a normal diet (CHOW), HFD alone, HFD plus <i>C. militaris</i> polysaccharides (CMP), or low/medium/high dosage of SeCMP for 8 weeks. Body weight, fat content, serum lipid, appetite hormone, lipid gene expression, inflammation cytokines, thermogenic protein, short-chain fatty acids (SCFAs), and gut microbiota structure of the mice were determined. Compared with HFD-fed mice, the serum triglyceride and low-density lipoprotein cholesterol (LDL-C) in the SeCMP-200 group were decreased by 51.5% and 44.1%, respectively. Furthermore, serum lipopolysaccharide-binding proteins (LBP), adiponectin level, and pro-inflammation gene expression in the colon and subcutaneous fat were inhibited, whereas anti-inflammation gene expression was improved, reflecting SeCMP-200 might mitigate obese-induced inflammation. Meanwhile, SeCMP-200 promoted satiety and thermogenesis of obese mice. It also significantly decreased gut bacteria, such as <i>Dorea</i>, <i>Lactobacillus</i>, <i>Clostridium</i>, <i>Ruminococcus,</i> that negatively correlated with obesity traits and increased mucosal beneficial bacteria <i>Akkermansia</i>. There was no significant difference between CMP and SeCMP-100 groups. Our results revealed a high dose of SeCMP could prevent HFD-induced dyslipidemia and gut microbiota dysbiosis and was potential to be used as functional foods.https://www.mdpi.com/2304-8158/10/10/2252<i>Cordyceps militaris</i>selenium-rich crude polysaccharideshigh-fat diet (HFD)dyslipidemiahypertriglyceridemiagut microbiota |
| spellingShingle | Minglei Yu Jin Yue Nan Hui Yuee Zhi Kashif Hayat Xijia Yang Dan Zhang Shaohua Chu Pei Zhou Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich <i>Cordyceps militaris</i> Polysaccharides on the High-Fat Diet-Fed Mice Model Foods <i>Cordyceps militaris</i> selenium-rich crude polysaccharides high-fat diet (HFD) dyslipidemia hypertriglyceridemia gut microbiota |
| title | Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich <i>Cordyceps militaris</i> Polysaccharides on the High-Fat Diet-Fed Mice Model |
| title_full | Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich <i>Cordyceps militaris</i> Polysaccharides on the High-Fat Diet-Fed Mice Model |
| title_fullStr | Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich <i>Cordyceps militaris</i> Polysaccharides on the High-Fat Diet-Fed Mice Model |
| title_full_unstemmed | Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich <i>Cordyceps militaris</i> Polysaccharides on the High-Fat Diet-Fed Mice Model |
| title_short | Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich <i>Cordyceps militaris</i> Polysaccharides on the High-Fat Diet-Fed Mice Model |
| title_sort | anti hyperlipidemia and gut microbiota community regulation effects of selenium rich i cordyceps militaris i polysaccharides on the high fat diet fed mice model |
| topic | <i>Cordyceps militaris</i> selenium-rich crude polysaccharides high-fat diet (HFD) dyslipidemia hypertriglyceridemia gut microbiota |
| url | https://www.mdpi.com/2304-8158/10/10/2252 |
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