A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco
Abscisic acid responsive element binding factors (ABFs) play crucial roles in plant responses to abiotic stress. However, little is known about the roles of ABFs in alpine subnival plants, which can survive under extreme environmental conditions. Here, we cloned and characterized an ABF1 homolog, Cb...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00699/full |
| _version_ | 1819022385427251200 |
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| author | Xiule Yue Guoyan Zhang Zhen Zhao Jinli Yue Xiaohong Pu Mengjun Sui Yi Zhan Yulan Shi Zhenyu Wang Guanghua Meng Zhixing Zhao Lizhe An |
| author_facet | Xiule Yue Guoyan Zhang Zhen Zhao Jinli Yue Xiaohong Pu Mengjun Sui Yi Zhan Yulan Shi Zhenyu Wang Guanghua Meng Zhixing Zhao Lizhe An |
| author_sort | Xiule Yue |
| collection | DOAJ |
| description | Abscisic acid responsive element binding factors (ABFs) play crucial roles in plant responses to abiotic stress. However, little is known about the roles of ABFs in alpine subnival plants, which can survive under extreme environmental conditions. Here, we cloned and characterized an ABF1 homolog, CbABF1, from the alpine subnival plant Chorispora bungeana. Expression of CbABF1 was induced by cold, drought, and abscisic acid. Subcellular localization analysis revealed that CbABF1 was located in the nucleus. Further, CbABF1 had transactivation activity, which was dependent on the N-terminal region containing 89 residues. A Snf1-related protein kinase, CbSnRK2.6, interacted with CbABF1 in yeast two-hybrid analysis and bimolecular fluorescence complementation assays. Transient expression assay revealed that CbSnRK2.6 enhanced the transactivation of CbABF1 on ABRE cis-element. We further found that heterologous expression of CbABF1 in tobacco improved plant tolerance to freezing and drought stress, in which the survival rates of the transgenic plants increased around 40 and 60%, respectively, compared with wild-type plants. Moreover, the transgenic plants accumulated less reactive oxygen species, accompanied by high activities of antioxidant enzymes and elevated expression of stress-responsive genes. Our results thus suggest that CbABF1 is a transcription factor that plays an important role in cold and drought tolerance and is a candidate gene in molecular breeding of stress-tolerant crops. |
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| format | Article |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T04:22:09Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7b6888aeb1b443699e7c3942916d92f82022-12-21T19:16:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-05-011010.3389/fpls.2019.00699418076A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in TobaccoXiule Yue0Guoyan Zhang1Zhen Zhao2Jinli Yue3Xiaohong Pu4Mengjun Sui5Yi Zhan6Yulan Shi7Zhenyu Wang8Guanghua Meng9Zhixing Zhao10Lizhe An11Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaMinistry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaCuiying Honors College, Lanzhou University, Lanzhou, ChinaMinistry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaMinistry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaMinistry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaMinistry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaExtreme Stress Resistance and Biotechnology Laboratory, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, ChinaInstitute of Tropical Agriculture and Forestry, Hainan University, Haikou, ChinaMinistry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaMinistry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaMinistry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, ChinaAbscisic acid responsive element binding factors (ABFs) play crucial roles in plant responses to abiotic stress. However, little is known about the roles of ABFs in alpine subnival plants, which can survive under extreme environmental conditions. Here, we cloned and characterized an ABF1 homolog, CbABF1, from the alpine subnival plant Chorispora bungeana. Expression of CbABF1 was induced by cold, drought, and abscisic acid. Subcellular localization analysis revealed that CbABF1 was located in the nucleus. Further, CbABF1 had transactivation activity, which was dependent on the N-terminal region containing 89 residues. A Snf1-related protein kinase, CbSnRK2.6, interacted with CbABF1 in yeast two-hybrid analysis and bimolecular fluorescence complementation assays. Transient expression assay revealed that CbSnRK2.6 enhanced the transactivation of CbABF1 on ABRE cis-element. We further found that heterologous expression of CbABF1 in tobacco improved plant tolerance to freezing and drought stress, in which the survival rates of the transgenic plants increased around 40 and 60%, respectively, compared with wild-type plants. Moreover, the transgenic plants accumulated less reactive oxygen species, accompanied by high activities of antioxidant enzymes and elevated expression of stress-responsive genes. Our results thus suggest that CbABF1 is a transcription factor that plays an important role in cold and drought tolerance and is a candidate gene in molecular breeding of stress-tolerant crops.https://www.frontiersin.org/article/10.3389/fpls.2019.00699/fullABFabiotic stresscryophyteChorispora bungeanacold stressdrought tolerance |
| spellingShingle | Xiule Yue Guoyan Zhang Zhen Zhao Jinli Yue Xiaohong Pu Mengjun Sui Yi Zhan Yulan Shi Zhenyu Wang Guanghua Meng Zhixing Zhao Lizhe An A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco Frontiers in Plant Science ABF abiotic stress cryophyte Chorispora bungeana cold stress drought tolerance |
| title | A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco |
| title_full | A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco |
| title_fullStr | A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco |
| title_full_unstemmed | A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco |
| title_short | A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco |
| title_sort | cryophyte transcription factor cbabf1 confers freezing and drought tolerance in tobacco |
| topic | ABF abiotic stress cryophyte Chorispora bungeana cold stress drought tolerance |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00699/full |
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