Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (<i>Betula pendula</i> Roth) and Black Birch (<i>Betula obscura</i> Kotula) Barks by FT-IR and HPLC-PDA
Silver birch, <i>Betula pendula</i> Roth, is one of the most common trees in Europe. Due to its content of many biologically active substances, it has long been used in medicine and cosmetics, unlike the rare black birch, <i>Betula obscura</i> Kotula. The aim of the study was...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/15/4633 |
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| author | Aleksandra Ostapiuk Łukasz Kurach Maciej Strzemski Jacek Kurzepa Anna Hordyjewska |
| author_facet | Aleksandra Ostapiuk Łukasz Kurach Maciej Strzemski Jacek Kurzepa Anna Hordyjewska |
| author_sort | Aleksandra Ostapiuk |
| collection | DOAJ |
| description | Silver birch, <i>Betula pendula</i> Roth, is one of the most common trees in Europe. Due to its content of many biologically active substances, it has long been used in medicine and cosmetics, unlike the rare black birch, <i>Betula obscura</i> Kotula. The aim of the study was therefore to compare the antioxidant properties of extracts from the inner and outer bark layers of both birch trees towards the L929 line treated with acetaldehyde. Based on the lactate dehydrogenase test and the MTT test, 10 and 25% concentrations of extracts were selected for the antioxidant evaluation. All extracts at tested concentrations reduced the production of hydrogen peroxide, superoxide anion radical, and 25% extract decreased malonic aldehyde formation in acetaldehyde-treated cells. The chemical composition of bark extracts was accessed by IR and HPLC-PDA methods and surprisingly, revealed a high content of betulin and lupeol in the inner bark extract of <i>B. obscura</i>. Furthermore, IR analysis revealed differences in the chemical composition of the outer bark between black and silver birch extracts, indicating that black birch may be a valuable source of numerous biologically active substances. Further experiments are required to evaluate their potential against neuroinflammation, cancer, viral infections, as well as their usefulness in cosmetology. |
| first_indexed | 2024-03-10T09:11:11Z |
| format | Article |
| id | doaj.art-7b6fee51f2f54abc91be1f0d6e3a02e6 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:11:11Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7b6fee51f2f54abc91be1f0d6e3a02e62023-11-22T06:01:00ZengMDPI AGMolecules1420-30492021-07-012615463310.3390/molecules26154633Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (<i>Betula pendula</i> Roth) and Black Birch (<i>Betula obscura</i> Kotula) Barks by FT-IR and HPLC-PDAAleksandra Ostapiuk0Łukasz Kurach1Maciej Strzemski2Jacek Kurzepa3Anna Hordyjewska4Department of Medical Chemistry, Medical University of Lublin, 4A Chodźki Str., 20-093 Lublin, PolandIndependent Laboratory of Behavioral Studies, Medical University of Lublin, 4A Chodźki Str., 20-093 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, 4A Chodźki Str., 20-093 Lublin, PolandDepartment of Medical Chemistry, Medical University of Lublin, 4A Chodźki Str., 20-093 Lublin, PolandDepartment of Medical Chemistry, Medical University of Lublin, 4A Chodźki Str., 20-093 Lublin, PolandSilver birch, <i>Betula pendula</i> Roth, is one of the most common trees in Europe. Due to its content of many biologically active substances, it has long been used in medicine and cosmetics, unlike the rare black birch, <i>Betula obscura</i> Kotula. The aim of the study was therefore to compare the antioxidant properties of extracts from the inner and outer bark layers of both birch trees towards the L929 line treated with acetaldehyde. Based on the lactate dehydrogenase test and the MTT test, 10 and 25% concentrations of extracts were selected for the antioxidant evaluation. All extracts at tested concentrations reduced the production of hydrogen peroxide, superoxide anion radical, and 25% extract decreased malonic aldehyde formation in acetaldehyde-treated cells. The chemical composition of bark extracts was accessed by IR and HPLC-PDA methods and surprisingly, revealed a high content of betulin and lupeol in the inner bark extract of <i>B. obscura</i>. Furthermore, IR analysis revealed differences in the chemical composition of the outer bark between black and silver birch extracts, indicating that black birch may be a valuable source of numerous biologically active substances. Further experiments are required to evaluate their potential against neuroinflammation, cancer, viral infections, as well as their usefulness in cosmetology.https://www.mdpi.com/1420-3049/26/15/4633<i>Betula pendula</i> Roth<i>Betula obscura</i> Kotulasilver birchblack birchpentacyclic triterpenes |
| spellingShingle | Aleksandra Ostapiuk Łukasz Kurach Maciej Strzemski Jacek Kurzepa Anna Hordyjewska Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (<i>Betula pendula</i> Roth) and Black Birch (<i>Betula obscura</i> Kotula) Barks by FT-IR and HPLC-PDA Molecules <i>Betula pendula</i> Roth <i>Betula obscura</i> Kotula silver birch black birch pentacyclic triterpenes |
| title | Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (<i>Betula pendula</i> Roth) and Black Birch (<i>Betula obscura</i> Kotula) Barks by FT-IR and HPLC-PDA |
| title_full | Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (<i>Betula pendula</i> Roth) and Black Birch (<i>Betula obscura</i> Kotula) Barks by FT-IR and HPLC-PDA |
| title_fullStr | Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (<i>Betula pendula</i> Roth) and Black Birch (<i>Betula obscura</i> Kotula) Barks by FT-IR and HPLC-PDA |
| title_full_unstemmed | Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (<i>Betula pendula</i> Roth) and Black Birch (<i>Betula obscura</i> Kotula) Barks by FT-IR and HPLC-PDA |
| title_short | Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (<i>Betula pendula</i> Roth) and Black Birch (<i>Betula obscura</i> Kotula) Barks by FT-IR and HPLC-PDA |
| title_sort | evaluation of antioxidative mechanisms in vitro and triterpenes composition of extracts from silver birch i betula pendula i roth and black birch i betula obscura i kotula barks by ft ir and hplc pda |
| topic | <i>Betula pendula</i> Roth <i>Betula obscura</i> Kotula silver birch black birch pentacyclic triterpenes |
| url | https://www.mdpi.com/1420-3049/26/15/4633 |
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