Biodefensive Based on <i>Piper nigrum</i> Essential Oil for Controlling of <i>Anopheles aquasalis</i> Larvae: Influence of Temperature (35 °C) and Preservatives
Considerable efforts have been spent on the development of biodefensives based on the encapsulation of essential oils for controlling of urban pests from their larval stage, especially as anopheline controlling agents. The larval source management of <i>Anopheles aquasalis</i> is importa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/11/1711 |
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| author | Ayná Caroline Marcião Vieira Sidney Gomes Azevedo Ramon Andrade Linhares Silvia Cássia Brandão Justiniano Grafe Oliveira Pontes Alessandra Ramos Lima Pedro Henrique Campelo Jaqueline de Araújo Bezerra Camila da Costa Pinto Henrique Duarte da Fonseca Filho Robert Saraiva Matos Ştefan Ţălu Vanderlei Salvador Bagnato Natalia Mayumi Inada Edgar Aparecido Sanches |
| author_facet | Ayná Caroline Marcião Vieira Sidney Gomes Azevedo Ramon Andrade Linhares Silvia Cássia Brandão Justiniano Grafe Oliveira Pontes Alessandra Ramos Lima Pedro Henrique Campelo Jaqueline de Araújo Bezerra Camila da Costa Pinto Henrique Duarte da Fonseca Filho Robert Saraiva Matos Ştefan Ţălu Vanderlei Salvador Bagnato Natalia Mayumi Inada Edgar Aparecido Sanches |
| author_sort | Ayná Caroline Marcião Vieira |
| collection | DOAJ |
| description | Considerable efforts have been spent on the development of biodefensives based on the encapsulation of essential oils for controlling of urban pests from their larval stage, especially as anopheline controlling agents. The larval source management of <i>Anopheles aquasalis</i> is important for malaria prevention. For this reason, this research proposes larvicidal biodefensives based on polymeric particles loaded with <i>Piper nigrum</i> essential oil, considering the influence of temperature (35 °C) and preservatives on the formulation stability. The biodefensive containing the preservative phenoxyethanol/methylisothiazolinone (PNE) resulted in 5 months of shelf-life storage with an Encapsulation Efficiency (EE%) of essential oil of 70%. The biodefensive PNE (containing 500 µg.mL<sup>−1</sup> of encapsulated essential oil) presented a polydisperse particle size distribution, ranging from D<sub>10</sub> = (127 ± 10) nm to D<sub>90</sub> = (472 ± 78) nm and a particle mean size of (236 ± 34) nm. The AFM images revealed a spherical morphology with an external surface almost regular and smooth. The controlled release of the essential oil was evaluated up to 72 h according to the Korsmeyer-Peppas mathematical model, confirming the anomalous transport (<i>n</i> = 0.64 in pH = 3 and pH = 10, and <i>n</i> = 0.65 in pH = 7). The total larvae mortality on the <i>in loco</i> bioassays was almost reached (92%) after 24 h. However, according to the <i>in vitro</i> bioassays applying the <i>in natura</i> essential oil alone, the concentration of 454 μg.mL<sup>−1</sup> resulted on the mortality of 70% of the larvae after 24 h. For this reason, the highest efficiency of the biodefensive PNE may be related to the encapsulation of essential oil, delivering the loaded particles more efficiently inside the larvae. From this perspective, the present study shows that a formulation based on <i>P. nigrum</i> essential oil may be taken into account in the integrated management of disease vector mosquitoes. |
| first_indexed | 2024-03-09T18:27:27Z |
| format | Article |
| id | doaj.art-7b719a847114424c813a7a37397b58d2 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T18:27:27Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-7b719a847114424c813a7a37397b58d22023-11-24T07:48:12ZengMDPI AGBiomolecules2218-273X2022-11-011211171110.3390/biom12111711Biodefensive Based on <i>Piper nigrum</i> Essential Oil for Controlling of <i>Anopheles aquasalis</i> Larvae: Influence of Temperature (35 °C) and PreservativesAyná Caroline Marcião Vieira0Sidney Gomes Azevedo1Ramon Andrade Linhares2Silvia Cássia Brandão Justiniano3Grafe Oliveira Pontes4Alessandra Ramos Lima5Pedro Henrique Campelo6Jaqueline de Araújo Bezerra7Camila da Costa Pinto8Henrique Duarte da Fonseca Filho9Robert Saraiva Matos10Ştefan Ţălu11Vanderlei Salvador Bagnato12Natalia Mayumi Inada13Edgar Aparecido Sanches14Laboratory of Nanostructured Polymers (NANOPOL), Federal University of Amazonas (UFAM), Manaus 69067-005, BrazilLaboratory of Nanostructured Polymers (NANOPOL), Federal University of Amazonas (UFAM), Manaus 69067-005, BrazilTropical Medicine Foundation Doctor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, BrazilTropical Medicine Foundation Doctor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, BrazilTropical Medicine Foundation Doctor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, BrazilSão Carlos Institute of Physics (IFSC), University of São Paulo (USP), São Carlos 13563-120, BrazilDepartment of Food Technology, Federal University of Viçosa (UFV), Viçosa 36570-900, BrazilFederal Institute of Education, Science and Technology of Amazonas (IFAM), Manaus 69020-120, BrazilFederal Institute of Education, Science and Technology of Amazonas (IFAM), Manaus 69020-120, BrazilLaboratory of Nanomaterials Synthesis and Nanoscopy (LSNN), Federal University of Amazonas (UFAM), Manaus 69067-005, BrazilAmazonian Materials Group, Federal University of Amapá (UNIFAP), Macapá 68903-419, BrazilThe Directorate of Research, Development and Innovation Management (DMCDI), Technical University of Cluj-Napoca, 15 Constantin Daicoviciu St., 400020 Cluj-Napoca, RomaniaSão Carlos Institute of Physics (IFSC), University of São Paulo (USP), São Carlos 13563-120, BrazilSão Carlos Institute of Physics (IFSC), University of São Paulo (USP), São Carlos 13563-120, BrazilLaboratory of Nanostructured Polymers (NANOPOL), Federal University of Amazonas (UFAM), Manaus 69067-005, BrazilConsiderable efforts have been spent on the development of biodefensives based on the encapsulation of essential oils for controlling of urban pests from their larval stage, especially as anopheline controlling agents. The larval source management of <i>Anopheles aquasalis</i> is important for malaria prevention. For this reason, this research proposes larvicidal biodefensives based on polymeric particles loaded with <i>Piper nigrum</i> essential oil, considering the influence of temperature (35 °C) and preservatives on the formulation stability. The biodefensive containing the preservative phenoxyethanol/methylisothiazolinone (PNE) resulted in 5 months of shelf-life storage with an Encapsulation Efficiency (EE%) of essential oil of 70%. The biodefensive PNE (containing 500 µg.mL<sup>−1</sup> of encapsulated essential oil) presented a polydisperse particle size distribution, ranging from D<sub>10</sub> = (127 ± 10) nm to D<sub>90</sub> = (472 ± 78) nm and a particle mean size of (236 ± 34) nm. The AFM images revealed a spherical morphology with an external surface almost regular and smooth. The controlled release of the essential oil was evaluated up to 72 h according to the Korsmeyer-Peppas mathematical model, confirming the anomalous transport (<i>n</i> = 0.64 in pH = 3 and pH = 10, and <i>n</i> = 0.65 in pH = 7). The total larvae mortality on the <i>in loco</i> bioassays was almost reached (92%) after 24 h. However, according to the <i>in vitro</i> bioassays applying the <i>in natura</i> essential oil alone, the concentration of 454 μg.mL<sup>−1</sup> resulted on the mortality of 70% of the larvae after 24 h. For this reason, the highest efficiency of the biodefensive PNE may be related to the encapsulation of essential oil, delivering the loaded particles more efficiently inside the larvae. From this perspective, the present study shows that a formulation based on <i>P. nigrum</i> essential oil may be taken into account in the integrated management of disease vector mosquitoes.https://www.mdpi.com/2218-273X/12/11/1711biodefensiveencapsulationessential oil<i>Piper nigrum</i><i>Anopheles aquasalis</i> |
| spellingShingle | Ayná Caroline Marcião Vieira Sidney Gomes Azevedo Ramon Andrade Linhares Silvia Cássia Brandão Justiniano Grafe Oliveira Pontes Alessandra Ramos Lima Pedro Henrique Campelo Jaqueline de Araújo Bezerra Camila da Costa Pinto Henrique Duarte da Fonseca Filho Robert Saraiva Matos Ştefan Ţălu Vanderlei Salvador Bagnato Natalia Mayumi Inada Edgar Aparecido Sanches Biodefensive Based on <i>Piper nigrum</i> Essential Oil for Controlling of <i>Anopheles aquasalis</i> Larvae: Influence of Temperature (35 °C) and Preservatives Biomolecules biodefensive encapsulation essential oil <i>Piper nigrum</i> <i>Anopheles aquasalis</i> |
| title | Biodefensive Based on <i>Piper nigrum</i> Essential Oil for Controlling of <i>Anopheles aquasalis</i> Larvae: Influence of Temperature (35 °C) and Preservatives |
| title_full | Biodefensive Based on <i>Piper nigrum</i> Essential Oil for Controlling of <i>Anopheles aquasalis</i> Larvae: Influence of Temperature (35 °C) and Preservatives |
| title_fullStr | Biodefensive Based on <i>Piper nigrum</i> Essential Oil for Controlling of <i>Anopheles aquasalis</i> Larvae: Influence of Temperature (35 °C) and Preservatives |
| title_full_unstemmed | Biodefensive Based on <i>Piper nigrum</i> Essential Oil for Controlling of <i>Anopheles aquasalis</i> Larvae: Influence of Temperature (35 °C) and Preservatives |
| title_short | Biodefensive Based on <i>Piper nigrum</i> Essential Oil for Controlling of <i>Anopheles aquasalis</i> Larvae: Influence of Temperature (35 °C) and Preservatives |
| title_sort | biodefensive based on i piper nigrum i essential oil for controlling of i anopheles aquasalis i larvae influence of temperature 35 °c and preservatives |
| topic | biodefensive encapsulation essential oil <i>Piper nigrum</i> <i>Anopheles aquasalis</i> |
| url | https://www.mdpi.com/2218-273X/12/11/1711 |
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