The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathway
Colorectal cancer is a prevalent malignant tumor, with liver metastases being the primary cause of death. The combination of inositol hexaphosphate (IP6) and inositol (INS) has been shown to inhibit colorectal cancer metastasis. We identified a correlation between CLDN23 expression and the occurrenc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623004711 |
| _version_ | 1797435807151161344 |
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| author | Haitao Wang Chuhui Wang Ning Yang Zhen Xu Yisa Han Feng Liu Xin Li Yang Song |
| author_facet | Haitao Wang Chuhui Wang Ning Yang Zhen Xu Yisa Han Feng Liu Xin Li Yang Song |
| author_sort | Haitao Wang |
| collection | DOAJ |
| description | Colorectal cancer is a prevalent malignant tumor, with liver metastases being the primary cause of death. The combination of inositol hexaphosphate (IP6) and inositol (INS) has been shown to inhibit colorectal cancer metastasis. We identified a correlation between CLDN23 expression and the occurrence of liver metastases in colorectal cancer patients. Following the transfection with the lentiviral vector (LV-CLDN23-RNAi), proteomic analysis of SW620 revealed significant alterations in the occludin, ZO-1, RhoA, and ROCK-1 genes expression by western blotting. We found that both SW620 and SW480 cells showed inhibited proliferation, invasion, and migration ability after the treatment with IP6 and INS. Additionally, this combination upregulated occludin and ZO-1, while downregulated CLDN23, RhoA, ROCK-1, and p-MLC2. In conclusion, the combination of IP6 and INS suppressed the proliferation, invasion, and migration of colorectal cancer cells and regulated CLDN23 expression by enhancing tight junction proteins, such as occludin and ZO1, via the RhoA /ROCK1 pathway. |
| first_indexed | 2024-03-09T10:53:26Z |
| format | Article |
| id | doaj.art-7b734f6938c54ec5ad33cd383b0fc87f |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-09T10:53:26Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-7b734f6938c54ec5ad33cd383b0fc87f2023-12-01T05:01:08ZengElsevierJournal of Functional Foods1756-46462023-12-01111105871The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathwayHaitao Wang0Chuhui Wang1Ning Yang2Zhen Xu3Yisa Han4Feng Liu5Xin Li6Yang Song7Department of Public Health, Qingdao University Medical College, Qingdao 266021, China; Affiliated Qingdao Central Hospital of Qingdao University, Qingdao Cancer Hospital, Shandong Province 266042, ChinaDepartment of Public Health, Qingdao University Medical College, Qingdao 266021, ChinaDepartment of Public Health, Qingdao University Medical College, Qingdao 266021, ChinaDepartment of Public Health, Qingdao University Medical College, Qingdao 266021, ChinaDepartment of Public Health, Qingdao University Medical College, Qingdao 266021, ChinaQingdaoHospitalof Traditional Chinese Medicine (Qingdao Hiser hospital), Qingdao, Shandong Province 266033, ChinaAffiliated Qingdao Central Hospital of Qingdao University, Qingdao Cancer Hospital, Shandong Province 266042, ChinaDepartment of Public Health, Qingdao University Medical College, Qingdao 266021, China; The Affiliated Cardiovascular Hospital of Qingdao University, China; Corresponding author at: Department of Public Health, Qingdao University Medical College, Qingdao 266021, China; The Affiliated Cardiovascular Hospital of Qingdao University, China (Y. Song).Colorectal cancer is a prevalent malignant tumor, with liver metastases being the primary cause of death. The combination of inositol hexaphosphate (IP6) and inositol (INS) has been shown to inhibit colorectal cancer metastasis. We identified a correlation between CLDN23 expression and the occurrence of liver metastases in colorectal cancer patients. Following the transfection with the lentiviral vector (LV-CLDN23-RNAi), proteomic analysis of SW620 revealed significant alterations in the occludin, ZO-1, RhoA, and ROCK-1 genes expression by western blotting. We found that both SW620 and SW480 cells showed inhibited proliferation, invasion, and migration ability after the treatment with IP6 and INS. Additionally, this combination upregulated occludin and ZO-1, while downregulated CLDN23, RhoA, ROCK-1, and p-MLC2. In conclusion, the combination of IP6 and INS suppressed the proliferation, invasion, and migration of colorectal cancer cells and regulated CLDN23 expression by enhancing tight junction proteins, such as occludin and ZO1, via the RhoA /ROCK1 pathway.http://www.sciencedirect.com/science/article/pii/S1756464623004711Colorectal cancer cellsClaudin23MetastasisInositol hexaphosphateInositolRhoA |
| spellingShingle | Haitao Wang Chuhui Wang Ning Yang Zhen Xu Yisa Han Feng Liu Xin Li Yang Song The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathway Journal of Functional Foods Colorectal cancer cells Claudin23 Metastasis Inositol hexaphosphate Inositol RhoA |
| title | The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathway |
| title_full | The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathway |
| title_fullStr | The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathway |
| title_full_unstemmed | The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathway |
| title_short | The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathway |
| title_sort | combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating cldn23 rhoa rock1 pathway |
| topic | Colorectal cancer cells Claudin23 Metastasis Inositol hexaphosphate Inositol RhoA |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623004711 |
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