Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, China
The endophytic bacteria in apple leaves from apple-producing areas of Yunnan, China were isolated and identified on the basis of bacterial colony morphology and nucleotide sequences of 16S <i>rRNA</i> and <i>rpoB</i> genes. The endophytic bacterial isolates with nitrogen, pho...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/11/991 |
| _version_ | 1797468091335049216 |
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| author | Xinlong Pan Pengbo He Cong Zhou Haichun Cun Pengfei He Shahzad Munir Yixin Wu Ayesha Ahmed Suhail Asad Jun Ma Yongcui Ma Yanming Zhang Keqiang Cao Baohua Kong Yueqiu He |
| author_facet | Xinlong Pan Pengbo He Cong Zhou Haichun Cun Pengfei He Shahzad Munir Yixin Wu Ayesha Ahmed Suhail Asad Jun Ma Yongcui Ma Yanming Zhang Keqiang Cao Baohua Kong Yueqiu He |
| author_sort | Xinlong Pan |
| collection | DOAJ |
| description | The endophytic bacteria in apple leaves from apple-producing areas of Yunnan, China were isolated and identified on the basis of bacterial colony morphology and nucleotide sequences of 16S <i>rRNA</i> and <i>rpoB</i> genes. The endophytic bacterial isolates with nitrogen, phosphorus, and potassium utilization abilities were screened by culturing on functional media. A total of 5709 isolates of culturable endophytic bacteria (CEB) were isolated from 30 apple leaf samples collected from different regions. A total of 39 CEB representative isolates were identified as <i>Bacillus velezensis</i>, <i>B. subtilis</i>, <i>B. licheniformis</i>, <i>B. safensis</i>, <i>B. pumilus</i>, and <i>Priestia megaterium</i>. Among them, <i>B. velezensis</i> and <i>B. subtilis</i> were the main CEB, accounting for 55.00% and 34.37%, respectively, which exhibited potential inhibition on not only the main apple disease pathogens of <i>Alternaria alternata</i>, <i>Valsa mali</i>, <i>Fusarium oxysporum</i>, and <i>Rosellinia necatrix</i>, but also some important and uncontrollable phytopathogens, including <i>F. oxysporum</i> f. sp. <i>cubense</i> that causes banana Fusarium wilt, and <i>Phytophthora nicotianae</i> that causes tobacco black shank. Among these isolated endophytic bacteria species, a total of 10 strains, including b3, b4, b16, b17, b20, and b23 of <i>B. subtilis</i>, b7, b24, and b28 of <i>B. licheniformis</i>, and b38 of <i>B. velezensis</i>, can fix nitrogen; 8 strains, including b7 and b28 of <i>B. licheniformis</i>, b5, b10, and b23 of <i>B. subtilis</i>, b8 of <i>B. safensis</i>, and b6 of <i>Priestia megaterium</i>, could dissolve inorganic phosphorus; 11 strains, including b9, b12, b14, b30, b34, and b43 of <i>B. velezensis</i>, b6 of <i>Priestia megaterium</i>, and b17, b18, b20, and b26 of <i>B. subtilis</i>, could degrade organic phosphorus; and 5 strains, including b4, b5, and b26 of <i>B. subtilis</i>, and b7 and b28 of <i>B. licheniformis</i> could dissolve potassium. These strains are valuable resources of endophytic bacteria that have adapted to the ecological environment of the Cold Plateau apple-production area and could be used as plant disease biocontrol agents and biofertilizers of crops. The culturable phyllosphere endophytes in apple leaves relate to geographic locations, apple varieties, and environment, providing the basis to explain the mechanisms underlying the establishment of apple endophyte diversity and may help to devise apple disease management strategies. |
| first_indexed | 2024-03-09T19:02:36Z |
| format | Article |
| id | doaj.art-7b7671e555104f1da486d9f9e4b7fe62 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T19:02:36Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-7b7671e555104f1da486d9f9e4b7fe622023-11-24T04:52:27ZengMDPI AGHorticulturae2311-75242022-10-0181199110.3390/horticulturae8110991Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, ChinaXinlong Pan0Pengbo He1Cong Zhou2Haichun Cun3Pengfei He4Shahzad Munir5Yixin Wu6Ayesha Ahmed7Suhail Asad8Jun Ma9Yongcui Ma10Yanming Zhang11Keqiang Cao12Baohua Kong13Yueqiu He14State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaHorticultural Crop Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaZhaotong College, Zhaotong 657000, ChinaEconomic Crop Research and Extension Station of Agricultural and Rural Bureau of Malong District, Qujing 655199, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071000, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaThe endophytic bacteria in apple leaves from apple-producing areas of Yunnan, China were isolated and identified on the basis of bacterial colony morphology and nucleotide sequences of 16S <i>rRNA</i> and <i>rpoB</i> genes. The endophytic bacterial isolates with nitrogen, phosphorus, and potassium utilization abilities were screened by culturing on functional media. A total of 5709 isolates of culturable endophytic bacteria (CEB) were isolated from 30 apple leaf samples collected from different regions. A total of 39 CEB representative isolates were identified as <i>Bacillus velezensis</i>, <i>B. subtilis</i>, <i>B. licheniformis</i>, <i>B. safensis</i>, <i>B. pumilus</i>, and <i>Priestia megaterium</i>. Among them, <i>B. velezensis</i> and <i>B. subtilis</i> were the main CEB, accounting for 55.00% and 34.37%, respectively, which exhibited potential inhibition on not only the main apple disease pathogens of <i>Alternaria alternata</i>, <i>Valsa mali</i>, <i>Fusarium oxysporum</i>, and <i>Rosellinia necatrix</i>, but also some important and uncontrollable phytopathogens, including <i>F. oxysporum</i> f. sp. <i>cubense</i> that causes banana Fusarium wilt, and <i>Phytophthora nicotianae</i> that causes tobacco black shank. Among these isolated endophytic bacteria species, a total of 10 strains, including b3, b4, b16, b17, b20, and b23 of <i>B. subtilis</i>, b7, b24, and b28 of <i>B. licheniformis</i>, and b38 of <i>B. velezensis</i>, can fix nitrogen; 8 strains, including b7 and b28 of <i>B. licheniformis</i>, b5, b10, and b23 of <i>B. subtilis</i>, b8 of <i>B. safensis</i>, and b6 of <i>Priestia megaterium</i>, could dissolve inorganic phosphorus; 11 strains, including b9, b12, b14, b30, b34, and b43 of <i>B. velezensis</i>, b6 of <i>Priestia megaterium</i>, and b17, b18, b20, and b26 of <i>B. subtilis</i>, could degrade organic phosphorus; and 5 strains, including b4, b5, and b26 of <i>B. subtilis</i>, and b7 and b28 of <i>B. licheniformis</i> could dissolve potassium. These strains are valuable resources of endophytic bacteria that have adapted to the ecological environment of the Cold Plateau apple-production area and could be used as plant disease biocontrol agents and biofertilizers of crops. The culturable phyllosphere endophytes in apple leaves relate to geographic locations, apple varieties, and environment, providing the basis to explain the mechanisms underlying the establishment of apple endophyte diversity and may help to devise apple disease management strategies.https://www.mdpi.com/2311-7524/8/11/991appleendophytic bacteria<i>Bacillus</i>low-latitude plateau |
| spellingShingle | Xinlong Pan Pengbo He Cong Zhou Haichun Cun Pengfei He Shahzad Munir Yixin Wu Ayesha Ahmed Suhail Asad Jun Ma Yongcui Ma Yanming Zhang Keqiang Cao Baohua Kong Yueqiu He Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, China Horticulturae apple endophytic bacteria <i>Bacillus</i> low-latitude plateau |
| title | Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, China |
| title_full | Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, China |
| title_fullStr | Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, China |
| title_full_unstemmed | Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, China |
| title_short | Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, China |
| title_sort | characterization and antagonistic effect of culturable apple phyllosphere endophytic bacteria from the cold plateau in yunnan china |
| topic | apple endophytic bacteria <i>Bacillus</i> low-latitude plateau |
| url | https://www.mdpi.com/2311-7524/8/11/991 |
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