COMPARATIVE IMMUNOGENICITY STUDIES OF ADJUVANTS FROM VARIOUS SOURCES AND WITH DIFFERENT MECHANISMS OF ACTION FOR INACTIVATED INFLUENZA VACCINES
Aim. Direct immunogenicity comparison of adjuvants from various sources and with different mechanisms of action for inactivated influenza vaccines. Materials and methods. Groups of mice were immunized intramuscularly twice with an inactivated whole-virion influenza vaccine based on A/ California/07/...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2015-12-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/view/14144 |
| _version_ | 1797782212103372800 |
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| author | M. I Chernikova O. S Kashirina Yu. M Vasiliev |
| author_facet | M. I Chernikova O. S Kashirina Yu. M Vasiliev |
| author_sort | M. I Chernikova |
| collection | DOAJ |
| description | Aim. Direct immunogenicity comparison of adjuvants from various sources and with different mechanisms of action for inactivated influenza vaccines. Materials and methods. Groups of mice were immunized intramuscularly twice with an inactivated whole-virion influenza vaccine based on A/ California/07/2009 X-179A (H1N1) strain. The following adjuvants were added to the vaccine (10 in total): aluminium hydroxide, oligonucleotide CpG, complete Freund’s adjuvant, poly(lactide-co-glycolide) microparticles, monophosphoryl lipid A and polyoxidonium, as well as 2 adjuvants based on characterized chitosan substances with different physical/chemical properties and 2 experimental complex formulations (a multi-component adjuvant and an oil-in-water emulsion based on squalene and tocopherol). Immunogenicity was determined by HAI and MN (MDCK) sera antibodies. Results. Different adjuvants increased immunogenicity of the vaccine against the homologous strain in varying patterns. Experimental complex formulations were the most immunogenic (antibody titer increase reached 48 - 96 times compared with unadjuvanted vaccines). Chitosan based adjuvants showed high immunogenicity. Not all the adjuvants significantly increased immunogenicity, and in some cases even an immunogenicity decrease was noted with the addition of certain adjuvants. Conclusion. Research and development of chitosan based adjuvants with characterization and standardization issues addressed, as well as complex adjuvants, both multi-component and emulsion based, are the most promising approaches that could lead to next generation vaccines against influenza and other human and animal infectious diseases. |
| first_indexed | 2024-03-13T00:07:49Z |
| format | Article |
| id | doaj.art-7b77a3b566c248709eb1a6ad4fb00bb4 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-13T00:07:49Z |
| publishDate | 2015-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-7b77a3b566c248709eb1a6ad4fb00bb42023-07-12T20:21:14ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132015-12-0192663712569COMPARATIVE IMMUNOGENICITY STUDIES OF ADJUVANTS FROM VARIOUS SOURCES AND WITH DIFFERENT MECHANISMS OF ACTION FOR INACTIVATED INFLUENZA VACCINESM. I Chernikova0O. S Kashirina1Yu. M Vasiliev2Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraAim. Direct immunogenicity comparison of adjuvants from various sources and with different mechanisms of action for inactivated influenza vaccines. Materials and methods. Groups of mice were immunized intramuscularly twice with an inactivated whole-virion influenza vaccine based on A/ California/07/2009 X-179A (H1N1) strain. The following adjuvants were added to the vaccine (10 in total): aluminium hydroxide, oligonucleotide CpG, complete Freund’s adjuvant, poly(lactide-co-glycolide) microparticles, monophosphoryl lipid A and polyoxidonium, as well as 2 adjuvants based on characterized chitosan substances with different physical/chemical properties and 2 experimental complex formulations (a multi-component adjuvant and an oil-in-water emulsion based on squalene and tocopherol). Immunogenicity was determined by HAI and MN (MDCK) sera antibodies. Results. Different adjuvants increased immunogenicity of the vaccine against the homologous strain in varying patterns. Experimental complex formulations were the most immunogenic (antibody titer increase reached 48 - 96 times compared with unadjuvanted vaccines). Chitosan based adjuvants showed high immunogenicity. Not all the adjuvants significantly increased immunogenicity, and in some cases even an immunogenicity decrease was noted with the addition of certain adjuvants. Conclusion. Research and development of chitosan based adjuvants with characterization and standardization issues addressed, as well as complex adjuvants, both multi-component and emulsion based, are the most promising approaches that could lead to next generation vaccines against influenza and other human and animal infectious diseases.https://microbiol.crie.ru/jour/article/view/14144influenzainactivated vaccinesimmunogenicityadjuvantsimmunoadjuvantschitosan |
| spellingShingle | M. I Chernikova O. S Kashirina Yu. M Vasiliev COMPARATIVE IMMUNOGENICITY STUDIES OF ADJUVANTS FROM VARIOUS SOURCES AND WITH DIFFERENT MECHANISMS OF ACTION FOR INACTIVATED INFLUENZA VACCINES Журнал микробиологии, эпидемиологии и иммунобиологии influenza inactivated vaccines immunogenicity adjuvants immunoadjuvants chitosan |
| title | COMPARATIVE IMMUNOGENICITY STUDIES OF ADJUVANTS FROM VARIOUS SOURCES AND WITH DIFFERENT MECHANISMS OF ACTION FOR INACTIVATED INFLUENZA VACCINES |
| title_full | COMPARATIVE IMMUNOGENICITY STUDIES OF ADJUVANTS FROM VARIOUS SOURCES AND WITH DIFFERENT MECHANISMS OF ACTION FOR INACTIVATED INFLUENZA VACCINES |
| title_fullStr | COMPARATIVE IMMUNOGENICITY STUDIES OF ADJUVANTS FROM VARIOUS SOURCES AND WITH DIFFERENT MECHANISMS OF ACTION FOR INACTIVATED INFLUENZA VACCINES |
| title_full_unstemmed | COMPARATIVE IMMUNOGENICITY STUDIES OF ADJUVANTS FROM VARIOUS SOURCES AND WITH DIFFERENT MECHANISMS OF ACTION FOR INACTIVATED INFLUENZA VACCINES |
| title_short | COMPARATIVE IMMUNOGENICITY STUDIES OF ADJUVANTS FROM VARIOUS SOURCES AND WITH DIFFERENT MECHANISMS OF ACTION FOR INACTIVATED INFLUENZA VACCINES |
| title_sort | comparative immunogenicity studies of adjuvants from various sources and with different mechanisms of action for inactivated influenza vaccines |
| topic | influenza inactivated vaccines immunogenicity adjuvants immunoadjuvants chitosan |
| url | https://microbiol.crie.ru/jour/article/view/14144 |
| work_keys_str_mv | AT michernikova comparativeimmunogenicitystudiesofadjuvantsfromvarioussourcesandwithdifferentmechanismsofactionforinactivatedinfluenzavaccines AT oskashirina comparativeimmunogenicitystudiesofadjuvantsfromvarioussourcesandwithdifferentmechanismsofactionforinactivatedinfluenzavaccines AT yumvasiliev comparativeimmunogenicitystudiesofadjuvantsfromvarioussourcesandwithdifferentmechanismsofactionforinactivatedinfluenzavaccines |