Phytogenically Synthesized Zinc Oxide Nanoparticles (ZnO-NPs) Potentially Inhibit the Bacterial Pathogens: In Vitro Studies
The usefulness of nanoparticles (NPs) in biological applications, such as nanomedicine, is becoming more widely acknowledged. Zinc oxide nanoparticles (ZnO-NPs) are a type of metal oxide nanoparticle with an extensive use in biomedicine. Here, ZnO-NPs were synthesized using <i>Cassia siamea<...
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MDPI AG
2023-05-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/5/452 |
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| author | Mo Ahamad Khan Showkat Ahmad Lone Mohammad Shahid Mohammad Tarique Zeyad Asad Syed Aquib Ehtram Abdallah M. Elgorban Meenakshi Verma Mohammad Danish |
| author_facet | Mo Ahamad Khan Showkat Ahmad Lone Mohammad Shahid Mohammad Tarique Zeyad Asad Syed Aquib Ehtram Abdallah M. Elgorban Meenakshi Verma Mohammad Danish |
| author_sort | Mo Ahamad Khan |
| collection | DOAJ |
| description | The usefulness of nanoparticles (NPs) in biological applications, such as nanomedicine, is becoming more widely acknowledged. Zinc oxide nanoparticles (ZnO-NPs) are a type of metal oxide nanoparticle with an extensive use in biomedicine. Here, ZnO-NPs were synthesized using <i>Cassia siamea</i> (L.) leaf extract and characterized using state-of-the-art techniques; UV–vis spectroscopy, XRD, FTIR, and SEM. At sub-minimum inhibitory concentration (MIC) levels, the ability of ZnO@<i>Cs</i>-NPs to suppress quorum-mediated virulence factors and biofilm formation against clinical MDR isolates (<i>Pseudomonas aeruginosa</i> PAO1 and <i>Chromobacterium violaceum</i> MCC-2290) was tested. The ½MIC of ZnO@<i>Cs</i>-NPs reduced violacein production by <i>C. violaceum</i>. Furthermore, ZnO@<i>Cs</i>-NPs sub-MIC significantly inhibited virulence factors such aspyoverdin, pyocyanin, elastase, exoprotease, rhamnolipid, and the swimming motility of <i>P. aeruginosa</i> PAO1 by 76.9, 49.0, 71.1, 53.3, 89.5, and 60%, respectively. Moreover, ZnO@<i>Cs</i>-NPs also showed wide anti-biofilm efficacy, inhibiting a maximum of 67 and 56% biofilms in <i>P. aeruginosa</i> and <i>C. violaceum</i>, respectively. In addition, ZnO@<i>Cs</i>-NPs suppressed extra polymeric substances (EPS) produced by isolates. Additionally, under confocal microscopy, propidium iodide-stained cells of <i>P. aeruginosa</i> and <i>C. violaceum</i> show ZnO@<i>Cs</i>-NP-induced impairment in membrane permeability, revealing strong anti-bacterial efficacy. This research demonstrates that newly synthesized ZnO@<i>Cs</i>-NPs demonstrate a strong efficacy against clinical isolates. In a nutshell, ZnO@<i>Cs</i>-NPs can be used as an alternative therapeutic agent for managing pathogenic infections. |
| first_indexed | 2024-03-11T03:15:28Z |
| format | Article |
| id | doaj.art-7b78723b35ba4c39966352f42736c190 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-11T03:15:28Z |
| publishDate | 2023-05-01 |
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| series | Toxics |
| spelling | doaj.art-7b78723b35ba4c39966352f42736c1902023-11-18T03:32:51ZengMDPI AGToxics2305-63042023-05-0111545210.3390/toxics11050452Phytogenically Synthesized Zinc Oxide Nanoparticles (ZnO-NPs) Potentially Inhibit the Bacterial Pathogens: In Vitro StudiesMo Ahamad Khan0Showkat Ahmad Lone1Mohammad Shahid2Mohammad Tarique Zeyad3Asad Syed4Aquib Ehtram5Abdallah M. Elgorban6Meenakshi Verma7Mohammad Danish8Department of Microbiology, Jawahar Lal Nehru Medical College (JNMC), Aligarh Muslim University, Aligarh 202002, IndiaDepartment of Microbiology, Government Medical College, Baramulla 19310, IndiaDepartment of Agricultural Microbiology, Faculty of Agriculture Science, Aligarh Muslim University, Aligarh 202002, IndiaDepartment of Agricultural Microbiology, Faculty of Agriculture Science, Aligarh Muslim University, Aligarh 202002, IndiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaLa Jolla Institute for Immunology, San Diego, CA 92037, USADepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaCentre of Research & Development, Department of Chemistry, Chandigarh University, Mohali 160055, IndiaDepartment of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, IndiaThe usefulness of nanoparticles (NPs) in biological applications, such as nanomedicine, is becoming more widely acknowledged. Zinc oxide nanoparticles (ZnO-NPs) are a type of metal oxide nanoparticle with an extensive use in biomedicine. Here, ZnO-NPs were synthesized using <i>Cassia siamea</i> (L.) leaf extract and characterized using state-of-the-art techniques; UV–vis spectroscopy, XRD, FTIR, and SEM. At sub-minimum inhibitory concentration (MIC) levels, the ability of ZnO@<i>Cs</i>-NPs to suppress quorum-mediated virulence factors and biofilm formation against clinical MDR isolates (<i>Pseudomonas aeruginosa</i> PAO1 and <i>Chromobacterium violaceum</i> MCC-2290) was tested. The ½MIC of ZnO@<i>Cs</i>-NPs reduced violacein production by <i>C. violaceum</i>. Furthermore, ZnO@<i>Cs</i>-NPs sub-MIC significantly inhibited virulence factors such aspyoverdin, pyocyanin, elastase, exoprotease, rhamnolipid, and the swimming motility of <i>P. aeruginosa</i> PAO1 by 76.9, 49.0, 71.1, 53.3, 89.5, and 60%, respectively. Moreover, ZnO@<i>Cs</i>-NPs also showed wide anti-biofilm efficacy, inhibiting a maximum of 67 and 56% biofilms in <i>P. aeruginosa</i> and <i>C. violaceum</i>, respectively. In addition, ZnO@<i>Cs</i>-NPs suppressed extra polymeric substances (EPS) produced by isolates. Additionally, under confocal microscopy, propidium iodide-stained cells of <i>P. aeruginosa</i> and <i>C. violaceum</i> show ZnO@<i>Cs</i>-NP-induced impairment in membrane permeability, revealing strong anti-bacterial efficacy. This research demonstrates that newly synthesized ZnO@<i>Cs</i>-NPs demonstrate a strong efficacy against clinical isolates. In a nutshell, ZnO@<i>Cs</i>-NPs can be used as an alternative therapeutic agent for managing pathogenic infections.https://www.mdpi.com/2305-6304/11/5/452green synthesisZnO-NPsMDR bacteriaanti-biofilmvirulence factorsEPS and membrane permeability |
| spellingShingle | Mo Ahamad Khan Showkat Ahmad Lone Mohammad Shahid Mohammad Tarique Zeyad Asad Syed Aquib Ehtram Abdallah M. Elgorban Meenakshi Verma Mohammad Danish Phytogenically Synthesized Zinc Oxide Nanoparticles (ZnO-NPs) Potentially Inhibit the Bacterial Pathogens: In Vitro Studies Toxics green synthesis ZnO-NPs MDR bacteria anti-biofilm virulence factors EPS and membrane permeability |
| title | Phytogenically Synthesized Zinc Oxide Nanoparticles (ZnO-NPs) Potentially Inhibit the Bacterial Pathogens: In Vitro Studies |
| title_full | Phytogenically Synthesized Zinc Oxide Nanoparticles (ZnO-NPs) Potentially Inhibit the Bacterial Pathogens: In Vitro Studies |
| title_fullStr | Phytogenically Synthesized Zinc Oxide Nanoparticles (ZnO-NPs) Potentially Inhibit the Bacterial Pathogens: In Vitro Studies |
| title_full_unstemmed | Phytogenically Synthesized Zinc Oxide Nanoparticles (ZnO-NPs) Potentially Inhibit the Bacterial Pathogens: In Vitro Studies |
| title_short | Phytogenically Synthesized Zinc Oxide Nanoparticles (ZnO-NPs) Potentially Inhibit the Bacterial Pathogens: In Vitro Studies |
| title_sort | phytogenically synthesized zinc oxide nanoparticles zno nps potentially inhibit the bacterial pathogens in vitro studies |
| topic | green synthesis ZnO-NPs MDR bacteria anti-biofilm virulence factors EPS and membrane permeability |
| url | https://www.mdpi.com/2305-6304/11/5/452 |
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