Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout
Summary Gastrin-releasing peptide (GRP), a neuropeptide initially isolated from porcine stomach, shares sequence similarity with bombesin. GRP and its receptors are present in the brains and peripheral tissues of several species of teleost fish, but little is known about the ventilatory and cardiova...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2013-07-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/2/9/960 |
| _version_ | 1818647159359143936 |
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| author | Jean-Claude Le Mével Frédéric Lancien Nagi Mimassi Marc Kermorgant J. Michael Conlon |
| author_facet | Jean-Claude Le Mével Frédéric Lancien Nagi Mimassi Marc Kermorgant J. Michael Conlon |
| author_sort | Jean-Claude Le Mével |
| collection | DOAJ |
| description | Summary
Gastrin-releasing peptide (GRP), a neuropeptide initially isolated from porcine stomach, shares sequence similarity with bombesin. GRP and its receptors are present in the brains and peripheral tissues of several species of teleost fish, but little is known about the ventilatory and cardiovascular effects of this peptide in these vertebrates. The goal of this study was to compare the central and peripheral actions of picomolar doses of trout GRP on ventilatory and cardiovascular variables in the unanesthetized rainbow trout. Compared to vehicle, intracerebroventricular (ICV) injection of GRP (1–50 pmol) significantly elevated the ventilation rate (ƒV) and the ventilation amplitude (VAMP), and consequently the total ventilation (VTOT). The maximum hyperventilatory effect of GRP (VTOT: +225%), observed at a dose of 50 pmol, was mostly due to its stimulatory action on VAMP (+170%) rather than ƒV (+20%). In addition, ICV GRP (50 pmol) produced a significant increase in mean dorsal aortic blood pressure (PDA) (+35%) and in heart rate (ƒH) (+25%). Intra-arterial injections of GRP (5–100 pmol) were without sustained effect on the ventilatory variables but produced sporadic and transient increases in ventilatory movement at doses of 50 and 100 pmol. At these doses, GRP elevated PDA by +20% but only the 50 pmol dose significantly increased HR (+15%). In conclusion, our study suggests that endogenous GRP within the brain of the trout may act as a potent neurotransmitter and/or neuromodulator in the regulation of cardio-ventilatory functions. In the periphery, endogenous GRP may act as locally-acting and/or circulating neurohormone with an involvement in vasoregulatory mechanisms. |
| first_indexed | 2024-12-17T00:58:06Z |
| format | Article |
| id | doaj.art-7b78da062c88492d9cfa57769cb8f7b3 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T00:58:06Z |
| publishDate | 2013-07-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-7b78da062c88492d9cfa57769cb8f7b32022-12-21T22:09:33ZengThe Company of BiologistsBiology Open2046-63902013-07-012996096710.1242/bio.2013555320135553Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized troutJean-Claude Le Mével0Frédéric Lancien1Nagi Mimassi2Marc Kermorgant3J. Michael Conlon4 Université Européenne de Bretagne, Université de Brest, INSERM UMR1101, Laboratoire de Traitement de l'Information Médicale, Laboratoire de Neurophysiologie, SFR ScInBioS, Faculté de Médecine et des Sciences de la Santé, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, CHU de Brest, France Université Européenne de Bretagne, Université de Brest, INSERM UMR1101, Laboratoire de Traitement de l'Information Médicale, Laboratoire de Neurophysiologie, SFR ScInBioS, Faculté de Médecine et des Sciences de la Santé, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, CHU de Brest, France Université Européenne de Bretagne, Université de Brest, INSERM UMR1101, Laboratoire de Traitement de l'Information Médicale, Laboratoire de Neurophysiologie, SFR ScInBioS, Faculté de Médecine et des Sciences de la Santé, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, CHU de Brest, France Université Européenne de Bretagne, Université de Brest, INSERM UMR1101, Laboratoire de Traitement de l'Information Médicale, Laboratoire de Neurophysiologie, SFR ScInBioS, Faculté de Médecine et des Sciences de la Santé, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, CHU de Brest, France Department of Biochemistry, College of Medicine and Health Sciences, United Arab Emirates University, 17666 Al Ain, United Arab Emirates Summary Gastrin-releasing peptide (GRP), a neuropeptide initially isolated from porcine stomach, shares sequence similarity with bombesin. GRP and its receptors are present in the brains and peripheral tissues of several species of teleost fish, but little is known about the ventilatory and cardiovascular effects of this peptide in these vertebrates. The goal of this study was to compare the central and peripheral actions of picomolar doses of trout GRP on ventilatory and cardiovascular variables in the unanesthetized rainbow trout. Compared to vehicle, intracerebroventricular (ICV) injection of GRP (1–50 pmol) significantly elevated the ventilation rate (ƒV) and the ventilation amplitude (VAMP), and consequently the total ventilation (VTOT). The maximum hyperventilatory effect of GRP (VTOT: +225%), observed at a dose of 50 pmol, was mostly due to its stimulatory action on VAMP (+170%) rather than ƒV (+20%). In addition, ICV GRP (50 pmol) produced a significant increase in mean dorsal aortic blood pressure (PDA) (+35%) and in heart rate (ƒH) (+25%). Intra-arterial injections of GRP (5–100 pmol) were without sustained effect on the ventilatory variables but produced sporadic and transient increases in ventilatory movement at doses of 50 and 100 pmol. At these doses, GRP elevated PDA by +20% but only the 50 pmol dose significantly increased HR (+15%). In conclusion, our study suggests that endogenous GRP within the brain of the trout may act as a potent neurotransmitter and/or neuromodulator in the regulation of cardio-ventilatory functions. In the periphery, endogenous GRP may act as locally-acting and/or circulating neurohormone with an involvement in vasoregulatory mechanisms.http://bio.biologists.org/content/2/9/960Central GRPIntracerebroventricular injectionHyperventilationHypertensionTeleost |
| spellingShingle | Jean-Claude Le Mével Frédéric Lancien Nagi Mimassi Marc Kermorgant J. Michael Conlon Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout Biology Open Central GRP Intracerebroventricular injection Hyperventilation Hypertension Teleost |
| title | Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout |
| title_full | Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout |
| title_fullStr | Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout |
| title_full_unstemmed | Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout |
| title_short | Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout |
| title_sort | central ventilatory and cardiovascular actions of trout gastrin releasing peptide grp in the unanesthetized trout |
| topic | Central GRP Intracerebroventricular injection Hyperventilation Hypertension Teleost |
| url | http://bio.biologists.org/content/2/9/960 |
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