Study the effect of a fixed orthodontic appliance on the oral microbial cavity
Background: Orthodontics is that branch of dentistry specialty dealing with diagnosis, prevention, and correction of malocclusion aiming to enhance oral health, dental function, and esthetics. The presence of a fixed orthodontic appliance within the oral cavity can alter the microbial balance and so...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Medical Journal of Babylon |
| Subjects: | |
| Online Access: | http://www.medjbabylon.org/article.asp?issn=1812-156X;year=2023;volume=20;issue=1;spage=168;epage=174;aulast=Al-nafaee |
| _version_ | 1797775422947065856 |
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| author | Asaad Kareem Al-nafaee Rasha Jasim Al-warid Kasem Ahmed Abeas |
| author_facet | Asaad Kareem Al-nafaee Rasha Jasim Al-warid Kasem Ahmed Abeas |
| author_sort | Asaad Kareem Al-nafaee |
| collection | DOAJ |
| description | Background: Orthodontics is that branch of dentistry specialty dealing with diagnosis, prevention, and correction of malocclusion aiming to enhance oral health, dental function, and esthetics. The presence of a fixed orthodontic appliance within the oral cavity can alter the microbial balance and so the composition of dental plaque. However, the presence of a fixed appliance alters the physiological characteristics of the oral cavity, causing impaired hygiene, a raise in dental plaque collection, and a delay in the spontaneous physiological processes of tooth cleaning that result in pathogenic bacteria colonization, which causes gingival inflammation, periodontal support degradation, and enamel surface alterations. Objective: The study’s objectives were isolated, identification, and molecular detection of microbes associated with orthodontic appliance patients. Materials and Methods: A total of 88 study samples (68 case; 20 control), 29 males and 59 females, aged 14–35 years, underwent fixed orthodontics during the initial stage of treatment. The samples were cultured on enrichment media and selective media, including mitis-salivarius agar, Mitis-Salivarius Bacitracin agar, Lactobacillus MRS agar, Eosin Methylene Blue agar, and Mannitol agar. They cultured in aerobic and anaerobic (10% CO2) conditions. Results: The positive samples were then purified and identified using morphological and biochemical tests. Among the microbial species gram-positive bacteria accounted for 82.3%, gram-negative bacteria 16.2%, and fungi 1.5%. Our results show that on one hand, gram-positive bacteria, Streptococcus mutans make higher percentage (48.5%) compared to other types. On the other hand, the Escherichia coli with 11.7% is the highest percentage of gram-negative bacteria. Conclusion: Bacteria colonization of orthodontic patients was found to be remarkably high compared to that of controls. Finally, the most common bacteria are S. mutans identify using PCR utilizing Sm.479.F/R primer combination. |
| first_indexed | 2024-03-12T22:35:32Z |
| format | Article |
| id | doaj.art-7b7adefc3c304cc693f7c7059c1f4bd9 |
| institution | Directory Open Access Journal |
| issn | 1812-156X 2312-6760 |
| language | English |
| last_indexed | 2024-03-12T22:35:32Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Babylon |
| spelling | doaj.art-7b7adefc3c304cc693f7c7059c1f4bd92023-07-21T12:46:31ZengWolters Kluwer Medknow PublicationsMedical Journal of Babylon1812-156X2312-67602023-01-0120116817410.4103/MJBL.MJBL_339_22Study the effect of a fixed orthodontic appliance on the oral microbial cavityAsaad Kareem Al-nafaeeRasha Jasim Al-waridKasem Ahmed AbeasBackground: Orthodontics is that branch of dentistry specialty dealing with diagnosis, prevention, and correction of malocclusion aiming to enhance oral health, dental function, and esthetics. The presence of a fixed orthodontic appliance within the oral cavity can alter the microbial balance and so the composition of dental plaque. However, the presence of a fixed appliance alters the physiological characteristics of the oral cavity, causing impaired hygiene, a raise in dental plaque collection, and a delay in the spontaneous physiological processes of tooth cleaning that result in pathogenic bacteria colonization, which causes gingival inflammation, periodontal support degradation, and enamel surface alterations. Objective: The study’s objectives were isolated, identification, and molecular detection of microbes associated with orthodontic appliance patients. Materials and Methods: A total of 88 study samples (68 case; 20 control), 29 males and 59 females, aged 14–35 years, underwent fixed orthodontics during the initial stage of treatment. The samples were cultured on enrichment media and selective media, including mitis-salivarius agar, Mitis-Salivarius Bacitracin agar, Lactobacillus MRS agar, Eosin Methylene Blue agar, and Mannitol agar. They cultured in aerobic and anaerobic (10% CO2) conditions. Results: The positive samples were then purified and identified using morphological and biochemical tests. Among the microbial species gram-positive bacteria accounted for 82.3%, gram-negative bacteria 16.2%, and fungi 1.5%. Our results show that on one hand, gram-positive bacteria, Streptococcus mutans make higher percentage (48.5%) compared to other types. On the other hand, the Escherichia coli with 11.7% is the highest percentage of gram-negative bacteria. Conclusion: Bacteria colonization of orthodontic patients was found to be remarkably high compared to that of controls. Finally, the most common bacteria are S. mutans identify using PCR utilizing Sm.479.F/R primer combination.http://www.medjbabylon.org/article.asp?issn=1812-156X;year=2023;volume=20;issue=1;spage=168;epage=174;aulast=Al-nafaeebacteriafixed orthodontic appliancemsb agarstreptococcus mutans |
| spellingShingle | Asaad Kareem Al-nafaee Rasha Jasim Al-warid Kasem Ahmed Abeas Study the effect of a fixed orthodontic appliance on the oral microbial cavity Medical Journal of Babylon bacteria fixed orthodontic appliance msb agar streptococcus mutans |
| title | Study the effect of a fixed orthodontic appliance on the oral microbial cavity |
| title_full | Study the effect of a fixed orthodontic appliance on the oral microbial cavity |
| title_fullStr | Study the effect of a fixed orthodontic appliance on the oral microbial cavity |
| title_full_unstemmed | Study the effect of a fixed orthodontic appliance on the oral microbial cavity |
| title_short | Study the effect of a fixed orthodontic appliance on the oral microbial cavity |
| title_sort | study the effect of a fixed orthodontic appliance on the oral microbial cavity |
| topic | bacteria fixed orthodontic appliance msb agar streptococcus mutans |
| url | http://www.medjbabylon.org/article.asp?issn=1812-156X;year=2023;volume=20;issue=1;spage=168;epage=174;aulast=Al-nafaee |
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