Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS
Hao-Zhen Zheng, Xiao Shen, Ying-Ying He, Xiang-Li Yan, Sheng-Xin Wang, Ai-Ming Yu, Li-Sheng Wang College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 51006, Guangdong, People’s Republic of ChinaCorrespondence: Li-Sheng Wang Tel +86 20-39358290Email wlis68@...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2020-07-01
|
| Series: | Drug Design, Development and Therapy |
| Subjects: | |
| Online Access: | https://www.dovepress.com/pharmacokinetic-analysis-of-huangqi-guizhi-wuwu-decoction-on-blood-and-peer-reviewed-article-DDDT |
| _version_ | 1819330546996609024 |
|---|---|
| author | Zheng HZ Shen X He YY Yan XL Wang SX Yu AM Wang LS |
| author_facet | Zheng HZ Shen X He YY Yan XL Wang SX Yu AM Wang LS |
| author_sort | Zheng HZ |
| collection | DOAJ |
| description | Hao-Zhen Zheng, Xiao Shen, Ying-Ying He, Xiang-Li Yan, Sheng-Xin Wang, Ai-Ming Yu, Li-Sheng Wang College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 51006, Guangdong, People’s Republic of ChinaCorrespondence: Li-Sheng Wang Tel +86 20-39358290Email wlis68@126.comObjective: The aim of our research was to analyze and compare the pharmacokinetics of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood and brain tissue of normal and cerebral ischemia-reperfusion injury rats by HPLC-MS/MS method.Methods: The blood and brain tissue samples of normal and middle cerebral artery occlusion (MCAO) rats were compared. The blood and brain tissue samples were collected by using microdialysis technique. The concentrations of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in blood and brain tissues were determined by the HPLC-MS/MS internal standard method.Results: Compared with the normal group, the model group after the administration of the Huangqi Guizhi Wuwu Decoction showed that Cmax blood, AUC0-t blood, and AUC0-inf blood of paeoniflorin were increased, CLblood, t1/2 brain, and Vbrain of paeoniflorin were decreased; Cmaxblood, AUC0-tblood, AUC0-infblood, and average residence time (MRTbrain) of calycosin-7-o-β-d-6-glucoside were decreased and the CLblood and Cmax brain of calycosin-7-o-β-d-6-glucoside were increased; Cmax blood of calycosin was decreased, Vblood and Vbrain of calycosin were increased; Cmax blood, AUC0-t blood, AUC0-inf blood, and MRTbrain of 6-gingerol were decreased, CLblood of 6-gingerol was increased.Conclusion: This method is simple, rapid, and sensitive. It is suitable for the pharmacokinetic study of Huangqi Guizhi Wuwu Decoction in the blood and brain tissue of rats. Cerebral ischemia-reperfusion injury increased the content of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood, affecting the clearance rate of paeoniflorin in the brain, the detention time of calycosin-7-o-β-d-6-glucoside and the 6-gingerol in the brain. In normal and cerebral ischemia-reperfusion rats, the content of paeoniflorin and 6-gingerol in the blood was higher than that in brain tissue, while the content of calycosin, calycosin-7-o-β-d-6-glucoside in the brain tissue was higher than that in blood, suggesting that calycosin and calycosin-7-o-β-d-6-glucoside have brain targeting properties.Keywords: Huangqi Guizhi Wuwu Decoction, microdialysis, paeoniflorin, calycosin-7-o-β-d-6-glucoside, calycosin, 6-gingerol, pharmacokinetics |
| first_indexed | 2024-12-24T14:00:15Z |
| format | Article |
| id | doaj.art-7b7f48419e1f4a26a0444b95db695c4c |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-12-24T14:00:15Z |
| publishDate | 2020-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-7b7f48419e1f4a26a0444b95db695c4c2022-12-21T16:52:29ZengDove Medical PressDrug Design, Development and Therapy1177-88812020-07-01Volume 142877288855512Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MSZheng HZShen XHe YYYan XLWang SXYu AMWang LSHao-Zhen Zheng, Xiao Shen, Ying-Ying He, Xiang-Li Yan, Sheng-Xin Wang, Ai-Ming Yu, Li-Sheng Wang College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 51006, Guangdong, People’s Republic of ChinaCorrespondence: Li-Sheng Wang Tel +86 20-39358290Email wlis68@126.comObjective: The aim of our research was to analyze and compare the pharmacokinetics of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood and brain tissue of normal and cerebral ischemia-reperfusion injury rats by HPLC-MS/MS method.Methods: The blood and brain tissue samples of normal and middle cerebral artery occlusion (MCAO) rats were compared. The blood and brain tissue samples were collected by using microdialysis technique. The concentrations of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in blood and brain tissues were determined by the HPLC-MS/MS internal standard method.Results: Compared with the normal group, the model group after the administration of the Huangqi Guizhi Wuwu Decoction showed that Cmax blood, AUC0-t blood, and AUC0-inf blood of paeoniflorin were increased, CLblood, t1/2 brain, and Vbrain of paeoniflorin were decreased; Cmaxblood, AUC0-tblood, AUC0-infblood, and average residence time (MRTbrain) of calycosin-7-o-β-d-6-glucoside were decreased and the CLblood and Cmax brain of calycosin-7-o-β-d-6-glucoside were increased; Cmax blood of calycosin was decreased, Vblood and Vbrain of calycosin were increased; Cmax blood, AUC0-t blood, AUC0-inf blood, and MRTbrain of 6-gingerol were decreased, CLblood of 6-gingerol was increased.Conclusion: This method is simple, rapid, and sensitive. It is suitable for the pharmacokinetic study of Huangqi Guizhi Wuwu Decoction in the blood and brain tissue of rats. Cerebral ischemia-reperfusion injury increased the content of paeoniflorin, calycosin, calycosin-7-o-β-d-6-glucoside, and 6-gingerol in the blood, affecting the clearance rate of paeoniflorin in the brain, the detention time of calycosin-7-o-β-d-6-glucoside and the 6-gingerol in the brain. In normal and cerebral ischemia-reperfusion rats, the content of paeoniflorin and 6-gingerol in the blood was higher than that in brain tissue, while the content of calycosin, calycosin-7-o-β-d-6-glucoside in the brain tissue was higher than that in blood, suggesting that calycosin and calycosin-7-o-β-d-6-glucoside have brain targeting properties.Keywords: Huangqi Guizhi Wuwu Decoction, microdialysis, paeoniflorin, calycosin-7-o-β-d-6-glucoside, calycosin, 6-gingerol, pharmacokineticshttps://www.dovepress.com/pharmacokinetic-analysis-of-huangqi-guizhi-wuwu-decoction-on-blood-and-peer-reviewed-article-DDDThuangqi guizhi wuwu decoctionmicrodialysispaeoniflorincalycosin-7-o-β-d-6-glucosidecalycosin6-gingerolpharmacokinetics |
| spellingShingle | Zheng HZ Shen X He YY Yan XL Wang SX Yu AM Wang LS Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS Drug Design, Development and Therapy huangqi guizhi wuwu decoction microdialysis paeoniflorin calycosin-7-o-β-d-6-glucoside calycosin 6-gingerol pharmacokinetics |
| title | Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS |
| title_full | Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS |
| title_fullStr | Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS |
| title_full_unstemmed | Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS |
| title_short | Pharmacokinetic Analysis of Huangqi Guizhi Wuwu Decoction on Blood and Brain Tissue in Rats with Normal and Cerebral Ischemia-Reperfusion Injury by Microdialysis with HPLC-MS/MS |
| title_sort | pharmacokinetic analysis of huangqi guizhi wuwu decoction on blood and brain tissue in rats with normal and cerebral ischemia reperfusion injury by microdialysis with hplc ms ms |
| topic | huangqi guizhi wuwu decoction microdialysis paeoniflorin calycosin-7-o-β-d-6-glucoside calycosin 6-gingerol pharmacokinetics |
| url | https://www.dovepress.com/pharmacokinetic-analysis-of-huangqi-guizhi-wuwu-decoction-on-blood-and-peer-reviewed-article-DDDT |
| work_keys_str_mv | AT zhenghz pharmacokineticanalysisofhuangqiguizhiwuwudecoctiononbloodandbraintissueinratswithnormalandcerebralischemiareperfusioninjurybymicrodialysiswithhplcmsms AT shenx pharmacokineticanalysisofhuangqiguizhiwuwudecoctiononbloodandbraintissueinratswithnormalandcerebralischemiareperfusioninjurybymicrodialysiswithhplcmsms AT heyy pharmacokineticanalysisofhuangqiguizhiwuwudecoctiononbloodandbraintissueinratswithnormalandcerebralischemiareperfusioninjurybymicrodialysiswithhplcmsms AT yanxl pharmacokineticanalysisofhuangqiguizhiwuwudecoctiononbloodandbraintissueinratswithnormalandcerebralischemiareperfusioninjurybymicrodialysiswithhplcmsms AT wangsx pharmacokineticanalysisofhuangqiguizhiwuwudecoctiononbloodandbraintissueinratswithnormalandcerebralischemiareperfusioninjurybymicrodialysiswithhplcmsms AT yuam pharmacokineticanalysisofhuangqiguizhiwuwudecoctiononbloodandbraintissueinratswithnormalandcerebralischemiareperfusioninjurybymicrodialysiswithhplcmsms AT wangls pharmacokineticanalysisofhuangqiguizhiwuwudecoctiononbloodandbraintissueinratswithnormalandcerebralischemiareperfusioninjurybymicrodialysiswithhplcmsms |