Genome-Wide Identification of 14-3-3 gene family reveals their diverse responses to abiotic stress by interacting with StABI5 in Potato (Solanum tuberosum L.)
The 14-3-3 genes are widely present in plants and participate in a wide range of cellular and physiological processes. In the current study, twelve 14-3-3s were identified from potato genome. According to phylogenetic evolutionary analysis, potato 14-3-3s were divided into ϵ and non-ϵ groups. Conser...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1090571/full |
| _version_ | 1797957304977457152 |
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| author | Qianqian Wang Chongchong Yan Yuying Fu Yu Wang Pengfei Jiang Yinyuan Ding Huajun Liao |
| author_facet | Qianqian Wang Chongchong Yan Yuying Fu Yu Wang Pengfei Jiang Yinyuan Ding Huajun Liao |
| author_sort | Qianqian Wang |
| collection | DOAJ |
| description | The 14-3-3 genes are widely present in plants and participate in a wide range of cellular and physiological processes. In the current study, twelve 14-3-3s were identified from potato genome. According to phylogenetic evolutionary analysis, potato 14-3-3s were divided into ϵ and non-ϵ groups. Conserved motif and gene structure analysis displayed a distinct class-specific divergence between the ϵ group and non-ϵ group. Multiple sequence alignments and three-dimensional structure analysis of 14-3-3 proteins indicated all the members contained nine conservative antiparallel α-helices. The majority of 14-3-3s had transcript accumulation in each detected potato tissue, implying their regulatory roles across all stages of potato growth and development. Numerous cis-acting elements related to plant hormones and abiotic stress response were identified in the promoter region of potato 14-3-3s, and the transcription levels of these genes fluctuated to different degrees under exogenous ABA, salt and drought stress, indicating that potato 14-3-3s may be involved in different hormone signaling pathways and abiotic stress responses. In addition, eight potato 14-3-3s were shown to interact with StABI5, which further demonstrated that potato 14-3-3s were involved in the ABA-dependent signaling pathway. This study provides a reference for the identification of the 14-3-3 gene family in other plants, and provides important clues for cloning potential candidates in response to abiotic stresses in potato. |
| first_indexed | 2024-04-11T00:02:17Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T00:02:17Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7b83523f667249518e43dab290a407e82023-01-09T15:05:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10905711090571Genome-Wide Identification of 14-3-3 gene family reveals their diverse responses to abiotic stress by interacting with StABI5 in Potato (Solanum tuberosum L.)Qianqian Wang0Chongchong Yan1Yuying Fu2Yu Wang3Pengfei Jiang4Yinyuan Ding5Huajun Liao6Key Laboratory of Horticultural Crop Germplasm innovation and Utilization, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, ChinaKey Laboratory of Horticultural Crop Germplasm innovation and Utilization, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, ChinaKey Laboratory of Horticultural Crop Germplasm innovation and Utilization, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, ChinaNational Engineering Laboratory of Crop Stress Resistance, School of Life Sciences, Anhui Agricultural University, Hefei, ChinaNational Engineering Laboratory of Crop Stress Resistance, School of Life Sciences, Anhui Agricultural University, Hefei, ChinaKey Laboratory of Horticultural Crop Germplasm innovation and Utilization, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, ChinaKey Laboratory of Horticultural Crop Germplasm innovation and Utilization, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, ChinaThe 14-3-3 genes are widely present in plants and participate in a wide range of cellular and physiological processes. In the current study, twelve 14-3-3s were identified from potato genome. According to phylogenetic evolutionary analysis, potato 14-3-3s were divided into ϵ and non-ϵ groups. Conserved motif and gene structure analysis displayed a distinct class-specific divergence between the ϵ group and non-ϵ group. Multiple sequence alignments and three-dimensional structure analysis of 14-3-3 proteins indicated all the members contained nine conservative antiparallel α-helices. The majority of 14-3-3s had transcript accumulation in each detected potato tissue, implying their regulatory roles across all stages of potato growth and development. Numerous cis-acting elements related to plant hormones and abiotic stress response were identified in the promoter region of potato 14-3-3s, and the transcription levels of these genes fluctuated to different degrees under exogenous ABA, salt and drought stress, indicating that potato 14-3-3s may be involved in different hormone signaling pathways and abiotic stress responses. In addition, eight potato 14-3-3s were shown to interact with StABI5, which further demonstrated that potato 14-3-3s were involved in the ABA-dependent signaling pathway. This study provides a reference for the identification of the 14-3-3 gene family in other plants, and provides important clues for cloning potential candidates in response to abiotic stresses in potato.https://www.frontiersin.org/articles/10.3389/fpls.2022.1090571/full14-3-3 gene familyabscisic acidabiotic stresspotatoStABI5 |
| spellingShingle | Qianqian Wang Chongchong Yan Yuying Fu Yu Wang Pengfei Jiang Yinyuan Ding Huajun Liao Genome-Wide Identification of 14-3-3 gene family reveals their diverse responses to abiotic stress by interacting with StABI5 in Potato (Solanum tuberosum L.) Frontiers in Plant Science 14-3-3 gene family abscisic acid abiotic stress potato StABI5 |
| title | Genome-Wide Identification of 14-3-3 gene family reveals their diverse responses to abiotic stress by interacting with StABI5 in Potato (Solanum tuberosum L.) |
| title_full | Genome-Wide Identification of 14-3-3 gene family reveals their diverse responses to abiotic stress by interacting with StABI5 in Potato (Solanum tuberosum L.) |
| title_fullStr | Genome-Wide Identification of 14-3-3 gene family reveals their diverse responses to abiotic stress by interacting with StABI5 in Potato (Solanum tuberosum L.) |
| title_full_unstemmed | Genome-Wide Identification of 14-3-3 gene family reveals their diverse responses to abiotic stress by interacting with StABI5 in Potato (Solanum tuberosum L.) |
| title_short | Genome-Wide Identification of 14-3-3 gene family reveals their diverse responses to abiotic stress by interacting with StABI5 in Potato (Solanum tuberosum L.) |
| title_sort | genome wide identification of 14 3 3 gene family reveals their diverse responses to abiotic stress by interacting with stabi5 in potato solanum tuberosum l |
| topic | 14-3-3 gene family abscisic acid abiotic stress potato StABI5 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1090571/full |
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