The tRNA thiolation-mediated translational control is essential for plant immunity
Plants have evolved sophisticated mechanisms to regulate gene expression to activate immune responses against pathogen infections. However, how the translation system contributes to plant immunity is largely unknown. The evolutionarily conserved thiolation modification of transfer RNA (tRNA) ensures...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2024-01-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/93517 |
| _version_ | 1797311922605916160 |
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| author | Xueao Zheng Hanchen Chen Zhiping Deng Yujing Wu Linlin Zhong Chong Wu Xiaodan Yu Qiansi Chen Shunping Yan |
| author_facet | Xueao Zheng Hanchen Chen Zhiping Deng Yujing Wu Linlin Zhong Chong Wu Xiaodan Yu Qiansi Chen Shunping Yan |
| author_sort | Xueao Zheng |
| collection | DOAJ |
| description | Plants have evolved sophisticated mechanisms to regulate gene expression to activate immune responses against pathogen infections. However, how the translation system contributes to plant immunity is largely unknown. The evolutionarily conserved thiolation modification of transfer RNA (tRNA) ensures efficient decoding during translation. Here, we show that tRNA thiolation is required for plant immunity in Arabidopsis. We identify a cgb mutant that is hyper-susceptible to the pathogen Pseudomonas syringae. CGB encodes ROL5, a homolog of yeast NCS6 required for tRNA thiolation. ROL5 physically interacts with CTU2, a homolog of yeast NCS2. Mutations in either ROL5 or CTU2 result in loss of tRNA thiolation. Further analyses reveal that both transcriptome and proteome reprogramming during immune responses are compromised in cgb. Notably, the translation of salicylic acid receptor NPR1 is reduced in cgb, resulting in compromised salicylic acid signaling. Our study not only reveals a regulatory mechanism for plant immunity but also uncovers an additional biological function of tRNA thiolation. |
| first_indexed | 2024-03-08T02:07:46Z |
| format | Article |
| id | doaj.art-7b86e34db196453dacb6ac993a188fa8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-08T02:07:46Z |
| publishDate | 2024-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7b86e34db196453dacb6ac993a188fa82024-02-13T15:30:41ZengeLife Sciences Publications LtdeLife2050-084X2024-01-011310.7554/eLife.93517The tRNA thiolation-mediated translational control is essential for plant immunityXueao Zheng0https://orcid.org/0000-0001-6204-7611Hanchen Chen1https://orcid.org/0009-0004-5323-6989Zhiping Deng2https://orcid.org/0000-0001-9663-3088Yujing Wu3Linlin Zhong4https://orcid.org/0000-0003-1908-1413Chong Wu5Xiaodan Yu6Qiansi Chen7https://orcid.org/0000-0003-2800-5703Shunping Yan8https://orcid.org/0000-0002-3665-1310Hubei Hongshan Laboratory, Wuhan, China; Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen, China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen, ChinaHubei Hongshan Laboratory, Wuhan, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen, China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaHubei Hongshan Laboratory, Wuhan, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen, China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, ChinaHubei Hongshan Laboratory, Wuhan, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen, China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen, ChinaHubei Hongshan Laboratory, Wuhan, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen, China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen, ChinaZhengzhou Tobacco Research Institute of CNTC, Zhengzhou, ChinaHubei Hongshan Laboratory, Wuhan, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen, China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen, ChinaPlants have evolved sophisticated mechanisms to regulate gene expression to activate immune responses against pathogen infections. However, how the translation system contributes to plant immunity is largely unknown. The evolutionarily conserved thiolation modification of transfer RNA (tRNA) ensures efficient decoding during translation. Here, we show that tRNA thiolation is required for plant immunity in Arabidopsis. We identify a cgb mutant that is hyper-susceptible to the pathogen Pseudomonas syringae. CGB encodes ROL5, a homolog of yeast NCS6 required for tRNA thiolation. ROL5 physically interacts with CTU2, a homolog of yeast NCS2. Mutations in either ROL5 or CTU2 result in loss of tRNA thiolation. Further analyses reveal that both transcriptome and proteome reprogramming during immune responses are compromised in cgb. Notably, the translation of salicylic acid receptor NPR1 is reduced in cgb, resulting in compromised salicylic acid signaling. Our study not only reveals a regulatory mechanism for plant immunity but also uncovers an additional biological function of tRNA thiolation.https://elifesciences.org/articles/93517plant immunitytranslationtRNA thiolationNPR1Arabidopsis |
| spellingShingle | Xueao Zheng Hanchen Chen Zhiping Deng Yujing Wu Linlin Zhong Chong Wu Xiaodan Yu Qiansi Chen Shunping Yan The tRNA thiolation-mediated translational control is essential for plant immunity eLife plant immunity translation tRNA thiolation NPR1 Arabidopsis |
| title | The tRNA thiolation-mediated translational control is essential for plant immunity |
| title_full | The tRNA thiolation-mediated translational control is essential for plant immunity |
| title_fullStr | The tRNA thiolation-mediated translational control is essential for plant immunity |
| title_full_unstemmed | The tRNA thiolation-mediated translational control is essential for plant immunity |
| title_short | The tRNA thiolation-mediated translational control is essential for plant immunity |
| title_sort | trna thiolation mediated translational control is essential for plant immunity |
| topic | plant immunity translation tRNA thiolation NPR1 Arabidopsis |
| url | https://elifesciences.org/articles/93517 |
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