Degradation of DDT by a Novel Bacterium, <i>Arthrobacter globiformis</i> DC-1: Efficacy, Mechanism and Comparative Advantage
A novel bacterium, <i>Arthrobacter globiformis</i> DC-1, capable of degrading DDT as its sole carbon and energy source, was isolated from DDT-contaminated agricultural soil. The bacterium can degrade up to 76.3% of the DDT at a concentration of 10 mg/L in the mineral salt medium (MSM) wi...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Water |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4441/15/15/2723 |
| _version_ | 1797585971960610816 |
|---|---|
| author | Xiaoxu Wang Belay Tafa Oba Hui Wang Qing Luo Jiaxin Liu Lanxin Tang Miao Yang Hao Wu Lina Sun |
| author_facet | Xiaoxu Wang Belay Tafa Oba Hui Wang Qing Luo Jiaxin Liu Lanxin Tang Miao Yang Hao Wu Lina Sun |
| author_sort | Xiaoxu Wang |
| collection | DOAJ |
| description | A novel bacterium, <i>Arthrobacter globiformis</i> DC-1, capable of degrading DDT as its sole carbon and energy source, was isolated from DDT-contaminated agricultural soil. The bacterium can degrade up to 76.3% of the DDT at a concentration of 10 mg/L in the mineral salt medium (MSM) within 1 day of incubation. The effects of various environmental conditions, such as the concentration of DDT, temperature, pH and additional carbon sources, on its growth and biodegrading capacity of DDT were investigated in the MSM. The <i>A. globiformis</i> DC-1 strain could efficiently grow and degrade DDT at a wide range of concentrations, with the maximum growth and degradation rate at 10 mg/LDDT, followed by inhibitory effects at higher concentrations (20 and 30 mg/LDDT). Mesophilic temperatures (25–30 °C) and a pH of 7–7.5 were the most suitable conditions for the growth and biodegradation. The presence of carbon sources significantly increased the growth of the DC-1 strain; however, degradation was inhibited in the present of glucose, sucrose and fructose, and peptone was determined to be the most appropriate carbon source for <i>A. globiformis</i> DC-1. The optimal DDT degradation (84.2%) was observed at 10 mg/LDDT, peptone as carbon source in pH 7.5 at 30 °C with 1 day of incubation. This strain could also degrade DDE, DDD and DDT simultaneously as the sole carbon and energy source, with degradation rates reaching 70.61%, 64.43% and 60.24% in 10 days, respectively. The biodegradation pathway by <i>A. globiformis</i> DC-1 revealed that DDT was converted to DDD and DDE via dechlorination and dehydrochlorination, respectively; subsequently, both DDD and DDE transformed to DDMU through further dechlorination, and finally, after ring opening, DDMU was mineralized to carbon dioxide. No intermediate metabolites accumulation was observed during the GC/MS analysis, demonstrating that the <i>A. globiformis</i> DC-1 strain can be used for the bioremediation of DDT residues in the environment. |
| first_indexed | 2024-03-11T00:13:34Z |
| format | Article |
| id | doaj.art-7b8b0421db75452f8ef2de4cad8576f6 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-11T00:13:34Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-7b8b0421db75452f8ef2de4cad8576f62023-11-18T23:46:53ZengMDPI AGWater2073-44412023-07-011515272310.3390/w15152723Degradation of DDT by a Novel Bacterium, <i>Arthrobacter globiformis</i> DC-1: Efficacy, Mechanism and Comparative AdvantageXiaoxu Wang0Belay Tafa Oba1Hui Wang2Qing Luo3Jiaxin Liu4Lanxin Tang5Miao Yang6Hao Wu7Lina Sun8Key Laboratory of Eco-Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang 110044, ChinaDepartment of Chemistry, College of Natural and Computational Sciences, Arba Minch University, Arba Minch 21, EthiopiaKey Laboratory of Eco-Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang 110044, ChinaKey Laboratory of Eco-Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang 110044, ChinaSichuan Provincial Engineering Laboratory of Monitoring and Control for Soil Erosion in Dry Valleys, China West Normal University, Nanchong 637009, ChinaSichuan Provincial Engineering Laboratory of Monitoring and Control for Soil Erosion in Dry Valleys, China West Normal University, Nanchong 637009, ChinaSichuan Provincial Engineering Laboratory of Monitoring and Control for Soil Erosion in Dry Valleys, China West Normal University, Nanchong 637009, ChinaKey Laboratory of Eco-Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang 110044, ChinaKey Laboratory of Eco-Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang 110044, ChinaA novel bacterium, <i>Arthrobacter globiformis</i> DC-1, capable of degrading DDT as its sole carbon and energy source, was isolated from DDT-contaminated agricultural soil. The bacterium can degrade up to 76.3% of the DDT at a concentration of 10 mg/L in the mineral salt medium (MSM) within 1 day of incubation. The effects of various environmental conditions, such as the concentration of DDT, temperature, pH and additional carbon sources, on its growth and biodegrading capacity of DDT were investigated in the MSM. The <i>A. globiformis</i> DC-1 strain could efficiently grow and degrade DDT at a wide range of concentrations, with the maximum growth and degradation rate at 10 mg/LDDT, followed by inhibitory effects at higher concentrations (20 and 30 mg/LDDT). Mesophilic temperatures (25–30 °C) and a pH of 7–7.5 were the most suitable conditions for the growth and biodegradation. The presence of carbon sources significantly increased the growth of the DC-1 strain; however, degradation was inhibited in the present of glucose, sucrose and fructose, and peptone was determined to be the most appropriate carbon source for <i>A. globiformis</i> DC-1. The optimal DDT degradation (84.2%) was observed at 10 mg/LDDT, peptone as carbon source in pH 7.5 at 30 °C with 1 day of incubation. This strain could also degrade DDE, DDD and DDT simultaneously as the sole carbon and energy source, with degradation rates reaching 70.61%, 64.43% and 60.24% in 10 days, respectively. The biodegradation pathway by <i>A. globiformis</i> DC-1 revealed that DDT was converted to DDD and DDE via dechlorination and dehydrochlorination, respectively; subsequently, both DDD and DDE transformed to DDMU through further dechlorination, and finally, after ring opening, DDMU was mineralized to carbon dioxide. No intermediate metabolites accumulation was observed during the GC/MS analysis, demonstrating that the <i>A. globiformis</i> DC-1 strain can be used for the bioremediation of DDT residues in the environment.https://www.mdpi.com/2073-4441/15/15/2723DDTbiodegradation<i>Arthrobacter globiformis</i>bioremediation |
| spellingShingle | Xiaoxu Wang Belay Tafa Oba Hui Wang Qing Luo Jiaxin Liu Lanxin Tang Miao Yang Hao Wu Lina Sun Degradation of DDT by a Novel Bacterium, <i>Arthrobacter globiformis</i> DC-1: Efficacy, Mechanism and Comparative Advantage Water DDT biodegradation <i>Arthrobacter globiformis</i> bioremediation |
| title | Degradation of DDT by a Novel Bacterium, <i>Arthrobacter globiformis</i> DC-1: Efficacy, Mechanism and Comparative Advantage |
| title_full | Degradation of DDT by a Novel Bacterium, <i>Arthrobacter globiformis</i> DC-1: Efficacy, Mechanism and Comparative Advantage |
| title_fullStr | Degradation of DDT by a Novel Bacterium, <i>Arthrobacter globiformis</i> DC-1: Efficacy, Mechanism and Comparative Advantage |
| title_full_unstemmed | Degradation of DDT by a Novel Bacterium, <i>Arthrobacter globiformis</i> DC-1: Efficacy, Mechanism and Comparative Advantage |
| title_short | Degradation of DDT by a Novel Bacterium, <i>Arthrobacter globiformis</i> DC-1: Efficacy, Mechanism and Comparative Advantage |
| title_sort | degradation of ddt by a novel bacterium i arthrobacter globiformis i dc 1 efficacy mechanism and comparative advantage |
| topic | DDT biodegradation <i>Arthrobacter globiformis</i> bioremediation |
| url | https://www.mdpi.com/2073-4441/15/15/2723 |
| work_keys_str_mv | AT xiaoxuwang degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage AT belaytafaoba degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage AT huiwang degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage AT qingluo degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage AT jiaxinliu degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage AT lanxintang degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage AT miaoyang degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage AT haowu degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage AT linasun degradationofddtbyanovelbacteriumiarthrobacterglobiformisidc1efficacymechanismandcomparativeadvantage |