Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat (Triticum aestivum L.)
Nitrogen (N) fertilizer application is essential for crop-plant growth and development. Identifying genetic loci associated with N-use efficiency (NUE) could increase wheat yields and reduce environmental pollution caused by overfertilization. We subjected a panel of 389 wheat accessions to N and ch...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-02-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514123001514 |
| _version_ | 1797289745241341952 |
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| author | Huawei Shi Weichong Wang Lifeng Gao Jirong Wu Chengmei Hu Huishu Yan Yugang Shi Ning Li Youzhi Ma Yongbin Zhou Zhaoshi Xu Jun Chen Wensi Tang Kai Chen Daizhen Sun Yuxiang Wu Ming Chen |
| author_facet | Huawei Shi Weichong Wang Lifeng Gao Jirong Wu Chengmei Hu Huishu Yan Yugang Shi Ning Li Youzhi Ma Yongbin Zhou Zhaoshi Xu Jun Chen Wensi Tang Kai Chen Daizhen Sun Yuxiang Wu Ming Chen |
| author_sort | Huawei Shi |
| collection | DOAJ |
| description | Nitrogen (N) fertilizer application is essential for crop-plant growth and development. Identifying genetic loci associated with N-use efficiency (NUE) could increase wheat yields and reduce environmental pollution caused by overfertilization. We subjected a panel of 389 wheat accessions to N and chlorate (a nitrate analog) treatments to identify quantitative trait loci (QTL) controlling NUE-associated traits at the wheat seedling stage. Genotyping the panel with a 660K single-nucleotide polymorphism (SNP) array, we identified 397 SNPs associated with N-sensitivity index and chlorate inhibition rate. These SNPs were merged into 49 QTL, of which eight were multi-environment stable QTL and 27 were located near previously reported QTL. A set of 135 candidate genes near the 49 QTL included TaBOX (F-box family protein) and TaERF (ethylene-responsive transcription factor). A Tabox mutant was more sensitive to low-N stress than the wild-type plant. We developed two functional markers for Hap 1, the favorable allele of TaBOX. |
| first_indexed | 2024-03-07T19:09:25Z |
| format | Article |
| id | doaj.art-7b8c0e18ab744488a6ae3f1b33dd0819 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-03-07T19:09:25Z |
| publishDate | 2024-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-7b8c0e18ab744488a6ae3f1b33dd08192024-03-01T05:06:22ZengKeAi Communications Co., Ltd.Crop Journal2214-51412024-02-01121222231Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat (Triticum aestivum L.)Huawei Shi0Weichong Wang1Lifeng Gao2Jirong Wu3Chengmei Hu4Huishu Yan5Yugang Shi6Ning Li7Youzhi Ma8Yongbin Zhou9Zhaoshi Xu10Jun Chen11Wensi Tang12Kai Chen13Daizhen Sun14Yuxiang Wu15Ming Chen16College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China; National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China; Corresponding authors.College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China; Corresponding authors.National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.Nitrogen (N) fertilizer application is essential for crop-plant growth and development. Identifying genetic loci associated with N-use efficiency (NUE) could increase wheat yields and reduce environmental pollution caused by overfertilization. We subjected a panel of 389 wheat accessions to N and chlorate (a nitrate analog) treatments to identify quantitative trait loci (QTL) controlling NUE-associated traits at the wheat seedling stage. Genotyping the panel with a 660K single-nucleotide polymorphism (SNP) array, we identified 397 SNPs associated with N-sensitivity index and chlorate inhibition rate. These SNPs were merged into 49 QTL, of which eight were multi-environment stable QTL and 27 were located near previously reported QTL. A set of 135 candidate genes near the 49 QTL included TaBOX (F-box family protein) and TaERF (ethylene-responsive transcription factor). A Tabox mutant was more sensitive to low-N stress than the wild-type plant. We developed two functional markers for Hap 1, the favorable allele of TaBOX.http://www.sciencedirect.com/science/article/pii/S2214514123001514WheatNUEGenome-wide association studyNitrogen sensitive indexChlorate inhibition rate |
| spellingShingle | Huawei Shi Weichong Wang Lifeng Gao Jirong Wu Chengmei Hu Huishu Yan Yugang Shi Ning Li Youzhi Ma Yongbin Zhou Zhaoshi Xu Jun Chen Wensi Tang Kai Chen Daizhen Sun Yuxiang Wu Ming Chen Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat (Triticum aestivum L.) Crop Journal Wheat NUE Genome-wide association study Nitrogen sensitive index Chlorate inhibition rate |
| title | Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat (Triticum aestivum L.) |
| title_full | Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat (Triticum aestivum L.) |
| title_fullStr | Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat (Triticum aestivum L.) |
| title_full_unstemmed | Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat (Triticum aestivum L.) |
| title_short | Genome-wide association study of seedling nitrogen-use efficiency-associated traits in common wheat (Triticum aestivum L.) |
| title_sort | genome wide association study of seedling nitrogen use efficiency associated traits in common wheat triticum aestivum l |
| topic | Wheat NUE Genome-wide association study Nitrogen sensitive index Chlorate inhibition rate |
| url | http://www.sciencedirect.com/science/article/pii/S2214514123001514 |
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