Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice
Introduction: Based on our previous findings, the treatment of stem cells alone or in combination with thyroid hormone (T3) and mild exercise could effectively reduce the risk of stroke damage in young mice. However, it is unclear whether this treatment is effective in aged or middle-aged mice. Ther...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2019-01-01
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| Series: | Basic and Clinical Neuroscience |
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| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-1293-1&slc_lang=en&sid=1 |
| _version_ | 1827333226051403776 |
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| author | Kobra Akhoundzadeh Abedin Vakili Hamid Reza Sameni |
| author_facet | Kobra Akhoundzadeh Abedin Vakili Hamid Reza Sameni |
| author_sort | Kobra Akhoundzadeh |
| collection | DOAJ |
| description | Introduction: Based on our previous findings, the treatment of stem cells alone or in combination with thyroid hormone (T3) and mild exercise could effectively reduce the risk of stroke damage in young mice. However, it is unclear whether this treatment is effective in aged or middle-aged mice. Therefore, this study designed to assess whether combination of Bone Marrow Stromal Cells (BMSCs) with T3 and mild treadmill exercise can decrease stroke complications in middle-aged mice.
Methods: Under laser Doppler flowmetry monitoring, transient focal cerebral ischemia was produced by right Middle Cerebral Artery Occlusion (MCAO) for 45 min followed by 7 days of reperfusion in middle-aged mice. BMSCs (1×105) were injected into the right cerebral ventricle 24 h after MCAO, followed by daily injection of triiodothyronine (T3) (20 µg/100 g/d SC) and 6 days of running on a treadmill. Infarct size, neurological function, apoptotic cells and expression levels of Glial Fibrillary Acidic Protein (GFAP) were evaluated 1 week after stroke.
Results: Post-ischemic treatment with BMSCs or with T3 and or mild treadmill exercise alone or in combination did not significantly change neurological function, infarct size, and apoptotic cells 7 days after ischemia in middle-aged mice (P>0.05). However, the expression of GFAP significantly reduced after treatment with BMSCs and or T3 (P<0.01).
Conclusion: Our findings indicate that post-stroke treatment BMSCs with exercise and thyroid hormone cannot reverse neuronal damage 7 days after ischemia in middle-aged mice. These findings further support that age is an important variable in stroke treatment |
| first_indexed | 2024-03-07T17:18:11Z |
| format | Article |
| id | doaj.art-7b8c5d7f0059453eb013a89d1c18411d |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:18:11Z |
| publishDate | 2019-01-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-7b8c5d7f0059453eb013a89d1c18411d2024-03-02T21:26:40ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422019-01-011017384Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged MiceKobra Akhoundzadeh0Abedin Vakili1Hamid Reza Sameni2 Physiology Research Center, Semnan University of Medical Sciences, Semnan, Iran. Physiology Research Center, Semnan University of Medical Sciences, Semnan, Iran. Neural Stem Cells Research Center, Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran. Introduction: Based on our previous findings, the treatment of stem cells alone or in combination with thyroid hormone (T3) and mild exercise could effectively reduce the risk of stroke damage in young mice. However, it is unclear whether this treatment is effective in aged or middle-aged mice. Therefore, this study designed to assess whether combination of Bone Marrow Stromal Cells (BMSCs) with T3 and mild treadmill exercise can decrease stroke complications in middle-aged mice. Methods: Under laser Doppler flowmetry monitoring, transient focal cerebral ischemia was produced by right Middle Cerebral Artery Occlusion (MCAO) for 45 min followed by 7 days of reperfusion in middle-aged mice. BMSCs (1×105) were injected into the right cerebral ventricle 24 h after MCAO, followed by daily injection of triiodothyronine (T3) (20 µg/100 g/d SC) and 6 days of running on a treadmill. Infarct size, neurological function, apoptotic cells and expression levels of Glial Fibrillary Acidic Protein (GFAP) were evaluated 1 week after stroke. Results: Post-ischemic treatment with BMSCs or with T3 and or mild treadmill exercise alone or in combination did not significantly change neurological function, infarct size, and apoptotic cells 7 days after ischemia in middle-aged mice (P>0.05). However, the expression of GFAP significantly reduced after treatment with BMSCs and or T3 (P<0.01). Conclusion: Our findings indicate that post-stroke treatment BMSCs with exercise and thyroid hormone cannot reverse neuronal damage 7 days after ischemia in middle-aged mice. These findings further support that age is an important variable in stroke treatmenthttp://bcn.iums.ac.ir/browse.php?a_code=A-10-1293-1&slc_lang=en&sid=1Cerebral ischemiaCombinationBone marrow stromal cellsThyroid hormoneExerciseApoptosisGlial fibrillary acidic proteinMiddle-agedMice |
| spellingShingle | Kobra Akhoundzadeh Abedin Vakili Hamid Reza Sameni Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice Basic and Clinical Neuroscience Cerebral ischemia Combination Bone marrow stromal cells Thyroid hormone Exercise Apoptosis Glial fibrillary acidic protein Middle-aged Mice |
| title | Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice |
| title_full | Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice |
| title_fullStr | Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice |
| title_full_unstemmed | Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice |
| title_short | Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice |
| title_sort | bone marrow stromal cells with exercise and thyroid hormone effect on post stroke injuries in middle aged mice |
| topic | Cerebral ischemia Combination Bone marrow stromal cells Thyroid hormone Exercise Apoptosis Glial fibrillary acidic protein Middle-aged Mice |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-1293-1&slc_lang=en&sid=1 |
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