Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique
Factor-mediated reprogramming of somatic cells towards pluripotency is a low-efficiency process during which only small subsets of cells are successfully reprogrammed. Previous analyses of the determinants of the reprogramming potential are based on average measurements across a large population of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2017-01-01
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| Series: | Open Biology |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160311 |
| _version_ | 1818873575154647040 |
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| author | Anastasia M. Yunusova Veniamin S. Fishman Gennady V. Vasiliev Nariman R. Battulin |
| author_facet | Anastasia M. Yunusova Veniamin S. Fishman Gennady V. Vasiliev Nariman R. Battulin |
| author_sort | Anastasia M. Yunusova |
| collection | DOAJ |
| description | Factor-mediated reprogramming of somatic cells towards pluripotency is a low-efficiency process during which only small subsets of cells are successfully reprogrammed. Previous analyses of the determinants of the reprogramming potential are based on average measurements across a large population of cells or on monitoring a relatively small number of single cells with live imaging. Here, we applied lentiviral genetic barcoding, a powerful tool enabling the identification of familiar relationships in thousands of cells. High-throughput sequencing of barcodes from successfully reprogrammed cells revealed a significant number of barcodes from related cells. We developed a computer model, according to which a probability of synchronous reprogramming of sister cells equals 10–30%. We conclude that the reprogramming success is pre-established in some particular cells and, being a heritable trait, can be maintained through cell division. Thus, reprogramming progresses in a deterministic manner, at least at the level of cell lineages. |
| first_indexed | 2024-12-19T12:56:53Z |
| format | Article |
| id | doaj.art-7b8cdcb407ea4efb9b5f11aed793dd2c |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-19T12:56:53Z |
| publishDate | 2017-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-7b8cdcb407ea4efb9b5f11aed793dd2c2022-12-21T20:20:22ZengThe Royal SocietyOpen Biology2046-24412017-01-017410.1098/rsob.160311160311Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding techniqueAnastasia M. YunusovaVeniamin S. FishmanGennady V. VasilievNariman R. BattulinFactor-mediated reprogramming of somatic cells towards pluripotency is a low-efficiency process during which only small subsets of cells are successfully reprogrammed. Previous analyses of the determinants of the reprogramming potential are based on average measurements across a large population of cells or on monitoring a relatively small number of single cells with live imaging. Here, we applied lentiviral genetic barcoding, a powerful tool enabling the identification of familiar relationships in thousands of cells. High-throughput sequencing of barcodes from successfully reprogrammed cells revealed a significant number of barcodes from related cells. We developed a computer model, according to which a probability of synchronous reprogramming of sister cells equals 10–30%. We conclude that the reprogramming success is pre-established in some particular cells and, being a heritable trait, can be maintained through cell division. Thus, reprogramming progresses in a deterministic manner, at least at the level of cell lineages.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160311induced pluripotent stem cellsreprogramming to pluripotencycellular barcodingcell fate decisions |
| spellingShingle | Anastasia M. Yunusova Veniamin S. Fishman Gennady V. Vasiliev Nariman R. Battulin Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique Open Biology induced pluripotent stem cells reprogramming to pluripotency cellular barcoding cell fate decisions |
| title | Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique |
| title_full | Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique |
| title_fullStr | Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique |
| title_full_unstemmed | Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique |
| title_short | Deterministic versus stochastic model of reprogramming: new evidence from cellular barcoding technique |
| title_sort | deterministic versus stochastic model of reprogramming new evidence from cellular barcoding technique |
| topic | induced pluripotent stem cells reprogramming to pluripotency cellular barcoding cell fate decisions |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160311 |
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