Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials
Xylella fastidiosa has multiple chromosomally encoded type II toxin-antitoxin (TA) systems, several of which have unknown functions in the bacterial lifecycle. In many bacterial species, type II TA systems are associated with bacterial stress survival under various detrimental environmental conditio...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2023-09-01
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| Series: | PhytoFrontiers |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-02-22-0009-R |
| _version_ | 1797330478734245888 |
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| author | Wei Wei Lindsey Burbank |
| author_facet | Wei Wei Lindsey Burbank |
| author_sort | Wei Wei |
| collection | DOAJ |
| description | Xylella fastidiosa has multiple chromosomally encoded type II toxin-antitoxin (TA) systems, several of which have unknown functions in the bacterial lifecycle. In many bacterial species, type II TA systems are associated with bacterial stress survival under various detrimental environmental conditions, and with influencing disease-associated phenotypes such as biofilm development and expression of virulence factors. Chromosomal TA systems are often found in genomic regions with evidence of phage insertion, pointing to potential acquisition of these elements by horizontal gene transfer. One of the TA systems found in X. fastidiosa, higBA, is transcriptionally expressed at higher levels at 15°C than at 28°C. However, a higBA deletion mutant (ΔhigBA) in X. fastidiosa subsp. fastidiosa Stag's Leap is not deficient in growth or viability under in vitro cold stress and is able to infect and cause disease in grapevines comparable to the wild-type strain. Grapevines infected with X. fastidiosa ΔhigBA recover from infection following cold treatment at 4°C at a similar rate to wild-type infected plants, suggesting that this TA system does not contribute significantly to X. fastidiosa survival at a low temperature in planta. Although a definitive function for HigBA in X. fastidiosa has yet to be determined, this study adds to a growing body of work describing the functions and expression dynamics of TA systems in plant pathogenic bacteria. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license. |
| first_indexed | 2024-03-08T07:20:25Z |
| format | Article |
| id | doaj.art-7b8f7c46c7d34ce5845f91cd4e337764 |
| institution | Directory Open Access Journal |
| issn | 2690-5442 |
| language | English |
| last_indexed | 2024-03-08T07:20:25Z |
| publishDate | 2023-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | PhytoFrontiers |
| spelling | doaj.art-7b8f7c46c7d34ce5845f91cd4e3377642024-02-02T23:52:49ZengThe American Phytopathological SocietyPhytoFrontiers2690-54422023-09-013233734610.1094/PHYTOFR-02-22-0009-RToxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse TrialsWei Wei0Lindsey Burbank1USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, Parlier, CAUSDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, Parlier, CAXylella fastidiosa has multiple chromosomally encoded type II toxin-antitoxin (TA) systems, several of which have unknown functions in the bacterial lifecycle. In many bacterial species, type II TA systems are associated with bacterial stress survival under various detrimental environmental conditions, and with influencing disease-associated phenotypes such as biofilm development and expression of virulence factors. Chromosomal TA systems are often found in genomic regions with evidence of phage insertion, pointing to potential acquisition of these elements by horizontal gene transfer. One of the TA systems found in X. fastidiosa, higBA, is transcriptionally expressed at higher levels at 15°C than at 28°C. However, a higBA deletion mutant (ΔhigBA) in X. fastidiosa subsp. fastidiosa Stag's Leap is not deficient in growth or viability under in vitro cold stress and is able to infect and cause disease in grapevines comparable to the wild-type strain. Grapevines infected with X. fastidiosa ΔhigBA recover from infection following cold treatment at 4°C at a similar rate to wild-type infected plants, suggesting that this TA system does not contribute significantly to X. fastidiosa survival at a low temperature in planta. Although a definitive function for HigBA in X. fastidiosa has yet to be determined, this study adds to a growing body of work describing the functions and expression dynamics of TA systems in plant pathogenic bacteria. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-02-22-0009-Rbacteriophageoverwinter recoveryPierce's diseasetoxin-antitoxin systemXylella fastidiosa |
| spellingShingle | Wei Wei Lindsey Burbank Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials PhytoFrontiers bacteriophage overwinter recovery Pierce's disease toxin-antitoxin system Xylella fastidiosa |
| title | Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials |
| title_full | Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials |
| title_fullStr | Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials |
| title_full_unstemmed | Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials |
| title_short | Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials |
| title_sort | toxin antitoxin system higba in xylella fastidiosa is expressed at low temperature but does not impact virulence or grapevine recovery in greenhouse trials |
| topic | bacteriophage overwinter recovery Pierce's disease toxin-antitoxin system Xylella fastidiosa |
| url | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-02-22-0009-R |
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